Sweet potato stem-nematode expansibility resistance identification method

A sweet potato stem nematode and identification method technology, applied in the field of agricultural plant protection, can solve the problems of lack of stem nematode disease resistance expansion characteristics, inconsistent horizontal and vertical expansion of nematodes, inability to effectively apply the identification of sweet potato stem nematode disease resistance expansion, etc., to achieve identification The effect of scientific method and simple operation process

Inactive Publication Date: 2010-07-28
JIANGSU XUZHOU SWEET POTATO RES CENT
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Problems solved by technology

However, many years of anti-expansion identification work has shown that the horizontal and vertical expansion of nematodes in potato blocks is inconsistent, and the control effect of the cross-section of the variety is only used to evaluate the resistance to stem nematode. Identification of resistance to expansion of stem nematode disease

Method used

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  • Sweet potato stem-nematode expansibility resistance identification method
  • Sweet potato stem-nematode expansibility resistance identification method
  • Sweet potato stem-nematode expansibility resistance identification method

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Embodiment

[0016] A kind of sweet potato stem nematode resistance expansion identification method is carried out according to the following steps:

[0017] (1) Participating in the preparation of the tested varieties, taking back the worm-bearing potato pieces infected with stem nematode from the field, and using the shallow dish method to separate the nematodes for subsequent use;

[0018] (2) For the varieties that need to be identified, prepare three medium-sized potato cubes for each variety, disinfect the surface with 75% alcohol after cleaning, put them in a sterilized plastic box for later use, and use the high-sensitivity variety Lizixiang for comparison;

[0019] (3) Nematode disinfection, filter the isolated nematodes with a 400-mesh sieve, wash them three times with sterile water, put them into a pre-prepared penicillin + streptomycin disinfectant, disinfect them for 5 minutes, and then wash them with sterile water Three times, made into a suspension of 200 / 1ml nematodes for ...

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Abstract

The invention relates to a method for appraising expansibility resistance of plant diseases and insect pests of crops, in particular to a method for appraising expansibility resistance of sweep stem nematode. The method is scientific, and simple in operation process, comprising: taking a plurality of strictly disinfected potato pieces; adopting a hole puncher with the diameter of 5 mm to perforate on the middle part of each potato piece; putting 200 eelworms into the hole by a disinfected pipetter, then chipping off a small section of potato strip and putting to the opening of the hole, usingolefin to seal the opening; putting the potato pieces into a disinfected plastic box, cultivating for 45 days at room temperature of 25 DEG C; then withdrawing appraising potato pieces, cutting from the perforated holes, measuring the lengths of the potato pieces and the lengths of expansibility of the eelworms from the cross sections of the potato pieces; calculating the ratios between the areasof the cross sections of the potato pieces and the areas of lesions; longitudinally cutting the potato pieces along the center points, measuring the lengths of the potato pieces and the lengths of the eelworms from the longitudinal sections of the potato pieces, and calculating the ratios between the lengths of the longitudinal sections of the potato pieces and the lengths of the lesions; respectively calculating the prevention performance of the cross section and the longitudinal section; classifying by the average prevention performance of the cross sections and the longitudinal sections; appraising the resistance property of the variety.

Description

technical field [0001] The invention relates to a method for identifying the resistance to expansion of crop diseases and insect pests, in particular to a method for identifying the resistance to expansion of sweet potato stem nematode disease, belonging to the field of agricultural plant protection. Background technique [0002] The sweet potato stem nematode Ditylenchus destructor is caused by the potato rot nematode, and it occurs seriously in Shandong, Jiangsu, Anhui and Henan. The disease leads to rotten pits during the storage period of sweet potatoes, and rotten beds during the seedling stage. After the sweet potatoes are damaged in the field stage, bran cores appear in the potato pieces. Generally, the diseased fields reduce production by 20%-50%, and even become extinct in serious cases. Continuous cropping and heavy cropping lead to the accumulation of nematodes, and the disease is getting worse year by year. It has become the most serious disease in sweet potato p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 谢逸萍马代夫李洪民李强王欣李秀英
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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