Identification method of resistance to sweet potato black mole disease

Abstract

The invention discloses a method for identifying resistance to sweet potato black mole disease, which belongs to methods for identifying resistance to crop diseases and insect pests. In mid-June, 30 robust seedlings of sweet potato varieties to be identified were cut by the high seedling cutting method in the seedbed, and divided into 3 replicates, with 10 plants in each replicate. When the potato seedlings were planted, the roots were dipped in a bacterial suspension with a concentration of 1.0×106 / ml, and the conventional methods were adopted for planting and daily field management. Harvest after 120 days of planting and store in sweet potato storage. During storage, the temperature is controlled at about 12-15°C and the relative humidity is at 80%-90%. After 150 days, the potato pieces were taken out, washed and dried, and the disease grade was divided according to the surface area of ​​the potato pieces with black mole lesions, and the disease index was calculated, and the resistance characteristics of the varieties were evaluated by the disease index. The identification method is scientific and effective, and the operation is simple. The test cycle covers the entire infection and expansion cycle of sweet potato black mole disease grown in the field and stored in winter. The identification results can fully reflect the resistance level of sweet potato varieties to black mole disease.

Description

technical field [0001] The invention relates to a method for identifying resistance to crop diseases and insect pests, in particular to a method for identifying resistance to sweet potato black mole disease, belonging to the field of agricultural plant protection. Background technique [0002] Sweetpotato mole disease (sweetpotato mole disease) is caused by the half-knowledge fungus subphylum Sweetpotato Alternaria (Monilochaetes infuscans Ell. et Halst. Ex Harter), and it occurs in every sweet potato producing area in my country. The disease generally only infects the potato skin, does not penetrate into the potato meat, has no significant impact on the yield, and has not attracted people's attention for a long time. Potato pieces are easy to lose moisture after being infected, and tend to shrink during storage, which affects quality and edible value. In addition, with the rise of the fresh sweet potato industry, the damage caused by black mole has become an important fact...

AI Technical Summary

Problems solved by technology

At present, the sweet potato varieties promoted in production are of good quality and high commodity value, but they are not resistant to black mole disease, and there is no report on the identification method of sweet potato black mole disease resistance, and it is impossible to make a conclusion on the resistance of sweet potato varieties to black mole disease. scientific evaluation

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