Tissue culture medium of phalaenopsis and propagation method of phalaenopsis

A tissue culture medium and phalaenopsis technology, applied in the field of plant tissue culture, can solve the problems of low reproductive coefficient of adventitious buds, affecting proliferation efficiency and quality of seedlings, etc. Effect

Pending Publication Date: 2022-04-08
赵英
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: aiming at the defect that the adventitious bud reproduction coefficient in the existing Phalaenopsis tissue culture technology is low, thus affecting the proliferation efficiency and seedling quality, to provide a method that can quickly and efficiently obtain a large number of high-quality seedlings Phalaenopsis tissue culture medium and propagation method

Method used

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  • Tissue culture medium of phalaenopsis and propagation method of phalaenopsis
  • Tissue culture medium of phalaenopsis and propagation method of phalaenopsis
  • Tissue culture medium of phalaenopsis and propagation method of phalaenopsis

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Experimental program
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Effect test

Embodiment 1-3

[0025] Embodiment 1-3 result

[0026]

[0027] 3) Proliferation culture of tissue cultured seedlings: Cut off the leaves and bases of the induced buds and inoculate them into the proliferation medium, at 25-28°C, light intensity 1500-2000Lx, light 12h / d, the multiplication factor> 5;

[0028] Described proliferation medium is 1 / 2MS+6-BA 3.5mg / L+KT1.0mg / L+NAA0.4mg / L+ albumen age 2g / L+coconut juice 100mL / L+ white sugar 20g / L, agar 5g / L, pH=5.5-5.6;

[0029]

[0030] 4) Cultivation of strong seedlings: Cut the proliferated buds and transfer them to the medium for strong seedlings. The culture temperature is 23-27°C, the light is 1500-2000Lx, the light is 10-12h per day, and the culture is 5-10 days;

[0031] The medium for strong seedlings is MS+6-BA2mg / L+protein age 2g / L+coconut juice 100mL / L+sugar 30g / L+agar 5g / L, pH=5.5~5.6;

[0032] 5) Rooting culture: Inoculate the Phalaenopsis seedlings cultivated by strong seedlings into the rooting medium, cultivate at 23-27°C, li...

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Abstract

The invention discloses a tissue culture medium for phalaenopsis. The culture medium comprises an induction culture medium, a proliferation culture medium, a seedling strengthening culture medium and a rooting culture medium, the invention relates to a tissue culture propagation method of phalaenopsis amabilis, which comprises the following steps: 1) material disinfection, 2) explant inoculation, 3) propagation culture of tissue culture seedlings, 4) strong seedling culture, 5) rooting culture, 6) acclimatization and domestication, and 7) transplanting. The method has the advantages that the multiplication culture period is short, the propagation coefficient is high, the transplanting survival rate can reach 99% or above, and the method can efficiently and rapidly provide high-quality phalaenopsis seedlings. By the adoption of the method, a large number of seedlings capable of being planted out of bottles can be obtained, the method is not affected by factors such as seasons, high-quality phalaenopsis seedlings can be efficiently and rapidly provided, and the good variety popularization speed can be increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture medium of Phalaenopsis and a propagation method thereof. Background technique [0002] Phalaenopsis [phalaenopsis] is a plant of the genus Phalaenopsis in the family Orchidaceae. It is a tropical aerial orchid, commonly known as "ocean orchid". Phalaenopsis is butterfly-like in shape, beautiful in shape, rich in color and long in flowering period. It is known as the "Queen of Orchids" among tropical orchids. Phalaenopsis is rich in species and widely distributed, from the Philippines and New Guinea in the east to northern Australia in the south, Sumatra in the west, and Taiwan, Yunnan, and western Sichuan in the north. There are original species (wild Phalaenopsis> exist, about 50 kinds, Among them, there are 7 species in my country, all of which are epiphytic orchids. The large-scale commercial cultivation of Phalaenopsis is very successful, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 赵英赵一卓徐航张志刚韩晓燕郑新国孔德智
Owner 赵英
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