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Culture medium for phalaenopsis amabilis tissue culture

A tissue culture, culture medium technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as serious browning degree

Inactive Publication Date: 2014-04-09
柳州赛特生物科技研发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the induction process of protocorms, the currently used medium is MS medium, but its browning degree is severe during use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A kind of Phalaenopsis tissue culture medium formula provided by the invention is as follows:

[0062] A kind of Phalaenopsis tissue culture medium of the present invention is improved on the basis of MS medium, and concrete formula is as follows:

[0063] KNO 3 : 1900mg / L;

[0064] NH 4 NO 3 : 1650mg / L;

[0065] K H 2 PO 4 : 170mg / L;

[0066] MgSO 4 ·7H 2 O: 370mg / L;

[0067] CaCl 2 2H 2 O: 440mg / L;

[0068] KI: 0.61mg / L;

[0069] h 3 BO 3 : 4.6mg / L;

[0070] MnSO 4 4H 2 O: 25.2mg / L;

[0071] ZnSO 4 ·7H 2 O: 7.1mg / L;

[0072] Na 2 MoO 4 2H 2 O: 0.21mg / L;

[0073] CuSO 4 ·5H 2 O: 0.025mg / L;

[0074] CoCl 2·6H 2 O: 0.025mg / L;

[0075] Na 2 EDTA: 37.25mg / L;

[0076] FeSO 4 ·7H 2 O: 27.85mg / L;

[0077] Inositol: 100mg / L;

[0078] Glycine: 2mg / L;

[0079] Thiamine hydrochloride: 0.1mg / L;

[0080] Pyridoxine hydrochloride: 0.5mg / L;

[0081] Niacin: 0.5mg / L;

[0082] Sucrose: 21g / L;

[0083] Agar: 5.1g / L;

[0084] Banana juice: 32g / ...

Embodiment 2

[0089] A kind of Phalaenopsis tissue culture medium formula provided by the invention is as follows:

[0090] A kind of Phalaenopsis tissue culture medium of the present invention is improved on the basis of MS medium, and concrete formula is as follows:

[0091] KNO 3 : 1900mg / L;

[0092] NH 4 NO 3 : 1650mg / L;

[0093] K H 2 PO 4 : 170mg / L;

[0094] MgSO 4 ·7H 2 O: 370mg / L;

[0095] CaCl 2 2H 2 O: 440mg / L;

[0096] KI: 0.61mg / L;

[0097] h 3 BO 3 : 4.6mg / L;

[0098] MnSO 4 4H 2 O: 25.0mg / L;

[0099] ZnSO 4 ·7H 2 O: 7.0mg / L;

[0100] Na 2 MoO 4 2H 2 O: 0.20mg / L;

[0101] CuSO 4 ·5H 2 O: 0.025mg / L;

[0102] CoCl 2 ·6H 2 O: 0.025mg / L;

[0103] Na 2 EDTA: 37.25mg / L;

[0104] FeSO 4 ·7H 2 O: 27.85mg / L;

[0105] Inositol: 100mg / L;

[0106] Glycine: 2mg / L;

[0107] Thiamine hydrochloride: 0.1mg / L;

[0108] Pyridoxine hydrochloride: 0.5mg / L;

[0109] Niacin: 0.5mg / L;

[0110] Sucrose: 20g / L;

[0111] Agar: 5.0g / L;

[0112] Banana juice: 30g...

Embodiment 3

[0117] A kind of Phalaenopsis tissue culture medium formula provided by the invention is as follows:

[0118] A kind of Phalaenopsis tissue culture medium of the present invention is improved on the basis of MS medium, and concrete formula is as follows:

[0119] KNO 3 : 1900mg / L;

[0120] NH 4 NO 3 : 1650mg / L;

[0121] K H 2 PO 4 : 170mg / L;

[0122] MgSO 4 ·7H 2 O: 370mg / L;

[0123] CaCl 2 2H 2 O: 440mg / L;

[0124] KI: 0.61mg / L;

[0125] h 3 BO 3 : 4.6mg / L;

[0126] MnSO 4 4H 2 O: 25.3mg / L;

[0127] ZnSO 4 ·7H 2 O: 7.2mg / L;

[0128] Na 2 MoO 4 2H 2 O: 0.22mg / L;

[0129] CuSO 4 ·5H 2 O: 0.025mg / L;

[0130] CoCl 2 ·6H 2 O: 0.025mg / L;

[0131] Na 2 EDTA: 37.25mg / L;

[0132] FeSO 4 ·7H 2 O: 27.85mg / L;

[0133] Inositol: 100mg / L;

[0134] Glycine: 2mg / L;

[0135] Thiamine hydrochloride: 0.1mg / L;

[0136] Pyridoxine hydrochloride: 0.5mg / L;

[0137] Niacin: 0.5mg / L;

[0138] Sucrose: 22g / L;

[0139] Agar: 5.2g / L;

[0140] Banana juice: 35g...

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Abstract

The invention provides a culture medium for phalaenopsis amabilis tissue culture. The culture medium comprises the following components: 1900 mg / L of KNO3; 1650 mg / L of ammonium nitrate; 170 mg / L of potassium dihydrogen phosphate; 370 mg / L of magnesium sulfate heptahydrate; 440 mg / L of calcium chloride dihydrate; 0.60-0.62 mg / L of KI; 4.5-4.8 mg / L of boric acid; 25.0-25.3 mg / L of manganese sulfate tetrahydrate; 7.0-7.2 mg / L of zinc sulfate heptahydrate; 0.20-0.22 mg / L of sodium molybdate dihydrate; 0.025 mg / L of copper sulfate pentahydrate; 0.025 mg / L of cobalt chloride hexahydrate; 37.25 mg / L of Na2.EDTA; 27.85 mg / L of ferrous sulfate heptahydrate; 100 mg / L of inositol; 2 mg / L of glycine; 0.1 mg / L of thiamine hydrochloride; 0.5 mg / L of pyridoxine hydrochloride; 0.5 mg / L of nicotinic acid; 20-22 g / L of sucrose; 5-5.2 g / L of agar; 30-35 g / L of a banana juice; 3-5 g / L of a pear juice; 0.45-0.48 g / L of activated carbon; and a solvent being deionized water. With application of the culture medium to culture phalaenopsis amabilis, the survival rate is more than 96%, the bacteria infection rate is 2.7-2.8%, and the browning degree of the culture medium is 30-40% lower than that of an MS culture medium.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a phalaenopsis tissue culture medium. Background technique [0002] Phalaenopsis amabilis (Phalaenopsis amabilis), Orchidaceae (Orchidaceae) Phalaenopsis (Phalaenopsis) perennial epiphytic herb, known as the "Queen of Orchids", because its flower shape is like a butterfly flying, colorful , light and elegant body, very popular in domestic and foreign flower markets, has always been the darling of consumers. In addition to potted plants, Phalaenopsis is also particularly suitable for cut flowers, and is a high-grade flower material for artistic flower arrangements. [0003] At present, the common method of Phalaenopsis factory production is to use tissue culture for vegetative propagation, that is, so-called clonal propagation, which has the advantages of fast propagation, large quantity and pure seedling varieties. Using explants such as Phalaenopsis pedic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李道强李培泰
Owner 柳州赛特生物科技研发中心
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