87 results about "Ph sensing" patented technology

Embodiments of the present invention are directed to an optical sensor capable of measuring two analytes simultaneously with a single indicator system. In preferred embodiments, the sensor comprises a fluorescent dye having acid and base forms that facilitate ratiometric pH sensing, wherein the dye is further associated with a glucose binding moiety and configured to generate a signal that varies in intensity with the concentration of glucose.

Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores −10 nm diameter can be reproducibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.

A device, system and method for in-vivo sensing (e.g., pH sensing, pressure sensing, or other sensing) may include more than one sensor receiving in-vivo data. The sensed data may be analyzed to, for example, determine a gradient or fluid flow. A device including multiple sensors may be held or immobilized in-vivo, and may detect a fluid flow or gradient moving past the sensors.

Embodiments of the present invention are directed to an optical sensor capable of measuring two analytes simultaneously with a single indicator system. In preferred embodiments, the sensor comprises a fluorescent dye having acid and base forms that facilitate ratiometric pH sensing, wherein the dye is further associated with a glucose binding moiety and configured to generate a signal that varies in intensity with the concentration of glucose.

Particular aspects provide novel devices and methods for accurately measuring total ammonia (NH3 plus NH4+) in a solution (e.g., freshwater and saltwater) by spatially proximate, simultaneous and continuous quantitative measurement of solution pH and ammonia. The devices overcome prior art inaccuracies relating to non-homogeneous sampling, and to spatial, temporal and thermal sampling discontinuities. Particular embodiments provide a combination pH and ammonia measuring device, comprising: a submersible member; a submersible non-bleeding ammonia-sensing portion attached to the submersible member and suitable to provide for a continuous visual indicator of solution ammonia concentration; a submersible non-bleeding pH-sensing portion attached to the submersible member and suitable to provide for a continuous visual indicator of solution pH; visual ammonia and pH indicator comparison means suitable for comparative quantitative determination of solution ammonia concentration and pH. Additional aspects provide novel ultra-sensitive devices and methods using same for measuring ammonia in air or solution.

The present invention relates to devices, methods, and kits for quickly and easily measuring and monitoring the pH of aquatic environments, such as lakes, streams, drinking waters, fisheries, aquariums, pools, hot-tubs, and spas. The current invention includes devices that are reusable and capable of monitoring the pH in a reversible fashion, thereby allowing continuous pH sensing without a need for the user to physically perform pH tests. Operation of the invention involves placing the device into the aquatic environment and optically detecting a color change in the membrane, for example, by looking at the device, when a pH measurement is desired. In addition, the device is both inexpensive and reusable, allowing the user to monitor the pH of various aquatic environment at minimal costs.

A sensing device includes a substrate having a source region and a drain region formed therein. A gate structure is formed over the substrate and includes a gate dielectric and a gate conductor. The gate conductor is formed on the gate dielectric and disposed between the source region and the drain region. A dielectric layer is formed over the substrate and has a depth configured to form a well over the gate conductor. A gate extension is formed in contact with or as part of the gate conductor and including a conductive material covering one or more surfaces of the well.

A sensing device includes a substrate having a source region and a drain region formed therein. A gate structure is formed over the substrate and includes a gate dielectric and a gate conductor. The gate conductor is formed on the gate dielectric and disposed between the source region and the drain region. A dielectric layer is formed over the substrate and has a depth configured to form a well over the gate conductor. A gate extension is formed in contact with or as part of the gate conductor and including a conductive material covering one or more surfaces of the well.

Particular aspects provide novel devices and methods for accurately measuring total ammonia (NH3 plus NH4+) in a solution (e.g., freshwater and saltwater) by spatially proximate, simultaneous and continuous quantitative measurement of solution pH and ammonia. The devices overcome prior art inaccuracies relating to non-homogeneous sampling, and to spatial, temporal and thermal sampling discontinuities. Particular embodiments provide a combination pH and ammonia measuring device, comprising: a submersible member; a submersible non-bleeding ammonia-sensing portion attached to the submersible member and suitable to provide for a continuous visual indicator of solution ammonia concentration; a submersible non-bleeding pH-sensing portion attached to the submersible member and suitable to provide for a continuous visual indicator of solution pH; visual ammonia and pH indicator comparison means suitable for comparative quantitative determination of solution ammonia concentration and pH. Additional aspects provide novel ultra-sensitive devices and methods using same for measuring ammonia in air or solution.

Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores −10 nm diameter can be reproducibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.

Embodiments of the present invention are directed to an optical sensor capable of measuring two analytes simultaneously with a single indicator system. In preferred embodiments, the sensor comprises a fluorescent dye having acid and base forms that facilitate ratiometric pH sensing, wherein the dye is further associated with a glucose binding moiety and configured to generate a signal that varies in intensity with the concentration of glucose.

This invention provides an extended gate ion-sensitive field effect transistor as a pH sensor for measuring the pH value of a solution under test. This invention also provides a pH sensing system comprising a separable and flexible pH sensor for measuring the pH value of a solution under test, wherein the transistor of the pH sensor can be prevented from direct contact with the solution.

The invention discloses a cyclizing metal iridium complex and an application thereof. The cyclizing metal iridium complex containing hydroxyl ligand or amino ligand has different luminescent properties in an entire pH range, the iridium complex containing the hydroxyl ligand is in off-on luminescent variation in a solution with the pH value of 0 to 14, the iridium complex containing the amino ligand is in off-on-off luminescent variation in the solution with the pH value of 0 to 14, and the luminescent property can be used for the pH sensing analysis test of the iridium complex. Partial low cell toxicity iridium complexes have obvious cell imaging and can be used for marking biomolecules.

The invention discloses an efficient online measurement system of a biological membrane photobioreactor. The efficient online measurement system comprises a biological membrane photobioreactor system, a biomass concentration and biological membrane thickness sensing system, an FBG (Fiber Bragg Grating) temperature sensing system and a hydrogen concentration and PH (Potential of Hydrogen) sensing system, wherein the biological membrane photobioreactor system comprises a photobioreactor, a reactor light source, a peristaltic pump, a constant-temperature water bath, a culture medium liquid storage bottle, a gas collection bottle, a gas-liquid separation bottle and the like; the biomass concentration and biological membrane thickness sensing system comprises an optical fiber light source, a light filter, multi-mode optical fibers, an optical branching device, an optical coupler, a biomass concentration sensing probe, a biological membrane thickness sensor and the like; the hydrogen concentration and PH sensing system comprises an H2 electrode, a pH reference electrode, a pH electrode and the like. As the sensors for the online measurement of biomass concentration, pH and hydrogen concentration are adopted in the online measurement system, the biomass concentration and the pH in the reactor can be regulated according to the real-time measurement results of the sensors, and the regulated hydrogen producing speed of the biological membrane reactor is improved.

The invention belongs to the technical field of the environment detection, and particularly discloses an application of a kjeldahl apparatus for measuring micromolecular harmful substance. The micromolecular harmful substance comprises volatile phenol and cyanide. The kjeldahl apparatus is provided with an alkali liquor opening, an acid liquor opening, a steam pipe and a water opening. The steam pipe is connected with the water opening. The steam pipe is internally provided with a pH sensing electrode. The application comprises the following steps: washing the pipeline of the kjeldahl apparatus, and adding a sample to be measured to the kjeldahl apparatus pipe, adding a indicator, adjusting the pH value, distilling, and collecting distillate for measuring. The distilling pretreatment process of the volatile phenol and cyanide in the water, soil and municipal sludge can be solved by the distilling device of the kjeldahl apparatus. The environmental and safe distilling method for measuring the cyanide in the water, soil and municipal sludge is established, and an experimenter is avoided to be hurt by the poisonous gas. The detection solution is simple and easily operated. The accuracy of the method is high, and the application prospect is extensive.

The invention discloses application of a near-infrared luminescent ruthenium complex to cell staining and pH sensing in a probe cell. The complex has almost no cytotoxicity at the staining concentration, and can stain a cell nucleus and cytoplasm. Through change of near-infrared luminescence intensity of the cell along with pH, the pH in the cell can be detected by a flow cytometry and a microscopic imaging technology.

A pH sensing bioreaction system is provided. The system includes a bioreaction container (51) having a plastic wall (50) and a pH sensor (40, 60, 80, 100, 120) attached to the plastic wall (50). The pH sensor (40, 60, 80, 100, 120) includes a sensor body (82) having a flange (76) that is sealingly attached to the plastic wall (50). The sensor body (82) has a reference electrolyte (142) therein and a first sensing element (152) disposed in the reference electrolyte (142). The first sensing element (152) is configured to contact both the reference electrolyte (142) and a sample solution (52) inside the bioreaction container. A second sensing element (62, 92, 148) is positionable into an interior of the bioreaction container (51). The pH sensor (40, 60, 80, 100, 120) has a plurality of configurations that include a booted configuration in which at least one sensing element is isolated from the interior of the bioreaction container (51), and a service configuration in which the at least one sensing element is fluidically coupled to the interior of the bioreaction container (51).

The invention relates to the technical field of fluorescence probes, in particular to an acid pH probe compound and a production method thereof. Triphenylamine compounds can be used as induced luminescence materials due to good fluorescence performance, and a schiff base structure has good coordination capability and protonation capability so that triphenylamine schiff base fluorescence compoundscan be used as pH sensing materials excellent in performance. However, the stability, especially in an acid water environment, of the schiff base structure under an acid condition is not ideal, and hydrolysis is prone to happen so that most of the schiff base structure pH probe compounds can hardly adapt to detection under strong acid water body conditions. The invention provides the acid pH fluorescence probe compound novel in structure; and the acid pH probe compound novel in structure has a good response effect in a pH range of 2-7, can adapt to strong acid water body environments, and hasimportant significance when applied for water body detection.

The invention provides a silver-plated gold nanorod side-by-side chiral oligomer and a preparation method and purposes thereof. The silver-plated gold nanorod side-by-side chiral oligomer comprises aplurality of parallel silver-plated gold nanorods; and cysteine is bonded to the surfaces of the silver-plated gold nanorods. The preparation method comprises the following steps that firstly, silvergrows on the surfaces of gold nanorods so as to form the silver-plated gold nanorods; secondly, a silver-plated gold nanorod side-by-side oligomer is formed under an electrostatic adsorption effect; and thirdly, the cysteine is added for reaction, and the silver-plated gold nanorod side-by-side chiral oligomer is formed. The silver-plated gold nanorod side-by-side chiral oligomer provided by the invention has a plasmonic circular dichroism (PCD) response and temperature and pH dependence, and can be used for the field of temperature and / or pH sensing.

The invention discloses a biological reaction kettle and a method for promoting proliferation and reddening of haematococcus pluvialis. The biological reaction kettle comprises a main body reaction kettle, wherein the main body reaction kettle is provided with a pH (potential of hydrogen) sensing head interface; the pH sensing head interface is connected with a pH controller; one end of the pH controller is connected with an industrial carbon dioxide bottle, and the other end of the pH controller is connected with the main body reaction kettle through an air intake adjusting valve; a filter is arranged at an outlet of the industrial carbon dioxide bottle; an LED (light emitting diode) lamp tube is arranged in the main body reaction kettle, and is connected with an LED driving controller; gas explosion stone is arranged at the bottom wall of the inner side of the main body reaction kettle, and is connected with a T-level filter through the air intake adjusting valve; the T-level filter is connected with an industrial air compressor; the LED lamp tube is connected with one end of a water cooling machine through the air intake adjusting valve, and the other end of the water cooling machine is connected with the LED lamp tube. The biological reaction kettle has the advantages that the problem of adverse influence by weather, light ray, pollution, environment temperature and the like in the existing traditional microalgae culture type is solved, and the growth speed of microalgae is greatly improved.

An in-body real-time photosensitive blood pH sensor is prepared from an ordinary optical fiber and an pH sensing film composed of the luminous electrochemical substance with high sensitivity to pH and the polymer containing phosphatide group through coating said pH sensing film on the terminal of said optical fiber. Its preparing process is also disclosed.

The invention relates to a method for fluorescently detecting the pH value through pH test paper and mainly sets forth a pH value detection method, different from a pH test paper color observation manner, based on the pH test paper. The method for fluorescently detecting the pH value through the pH test paper comprises the steps of firstly acquiring a relation function between fluorescence spectrum parameters of the pH test paper and a pH value of liquid as a fluorescence pH sensing equation, dipping the same type of the pH test paper into to-be-tested liquid with an unknown pH value, drying the moistened and developed pH test paper, measuring the fluorescence spectrum parameters of the pH test paper, and substituting the fluorescence spectrum parameters into the relation function between the fluorescence spectrum parameters of the pH test paper and the pH value of the liquid, so as to obtain the pH value of the to-be-tested liquid. The method for fluorescently detecting the pH value through the pH test paper has the advantages that the pH test paper used in the testing process can be preserved for a long time and repeatedly verified; the detection process can be far from a site in which corrosive liquid exists; compared with a manner of judging the colors of the moistened test paper, the pH detection precision of the method is relatively high.

The invention provides a preparation method and application for fluorescent copper nanoclusters. The preparation method of the fluorescent copper nanoclusters comprises the following steps of uniformly mixing 1 part of a copper nitrate solution with the concentration being 0.05-0.2mol / L and 5-20 parts of a histidine aqueous solution with the concentration being 0.05-0.2mol / L at a room temperatureby volume parts, putting a mixture in a 300-700W microwave oven for heating for 10-20 min, and dissolving a reaction product with water to obtain a green fluorescent copper nanocluster aqueous solution. The preparation process disclosed by the invention is simple and rapid and has the advantages of cheap and available raw materials, excellent fluorescence performance, good biocompatibility and thelike; and the prepared copper nanocluster aqueous solution is good in water solubility and stability and high in fluorescence intensity, is non-toxic and can be applied to detection of pH sensing andpicric acid.

The present invention discloses a field effect transistor structure-based pH sensing device and a manufacturing method thereof. The manufacturing method is as follows: firstly, a front gate oxide layer and a gate layer are deposited on the surface of a semiconductor layer of a semiconductor substrate on an insulating layer, a gate electrode structure is formed by etching of the front gate oxide layer and the gate layer; secondly, a source-drain structure is formed by doping of the semiconductor layer of the semiconductor substrate on the insulating layer on both sides of the gate electrode structure, and the gate electrode structure and the source-drain structure together form a field-effect transistor structure; and further, photolithography is performed on the back surface of a silicon substrate on the insulating layer, a supporting substrate and a buried oxide layer are etched until the lower surface of the semiconductor layer, and a back gate oxide layer is deposited on the lower surface of the etched semiconductor layer to complete the preparation of the pH sensing device. The method utilizes the contact between a solution to be tested and a back gate of the field-effect transistor to form a double-electric-layer structure on the surface of the back gate, by changing of the Zeta potential of the double-electric-layer on the surface of the back gate in different pH solutions, the threshold voltage of the field-effect transistor can be changed, the change of the resistance of the sensing device under a fixed bias voltage can be further led, the pH value of the solution can be tested.

The invention relates to a composition, method and apparatus for determining the sequence of a nucleic acid strand utilizing a pH-sensing agent.

Described is a versatile surface modification approach to, for example, modularly and orthogonally functionalize nanoparticles (NPs) such as, for example, PEGylated nanoparticles, ith various types of different functional ligands (functional groups) on the NP surface. It enables the synthesis of, for example, penta-functional PEGylated nanoparticles integrating a variety of properties into a single NP, e.g., fluorescence detection, specific cell targeting, radioisotope chelating / labeling, ratiometric pH sensing, and drug delivery, while the overall NP size remains, for example, below 10 nm.

The invention provides a pH electrochemical sensor based on an aniline oligomer / graphene composite material. The sensor comprises a pH sensing electrode; the surface of the pH sensing electrode is modified by the aniline oligomer / graphene composite material; and the composite material is formed by coating the surface of the electrode with a composite material dispersion solution and then carryingout drying. The composite material modified electrode is simple in preparation process, small in size, low in production cost and convenient to detect, and does not need special treatment before and after being used; and the obtained pH electrochemical sensor can be used as a pH sensor to dynamically detect the change of the pH value, and can be applied to dynamic process detection of biological or chemical reactions. Graphene is introduced into aniline oligomer to obviously improve the pH sensitivity of the electrode; and meanwhile, compared with a pure aniline oligomer modified electrode, the detection sensitivity of the composite material modified electrode is improved by nearly three times from 2.60 [mu]A.pH-1.cm<-2> to 8.06 [mu]A.pH-1.cm<-2>, and the detection range is expanded from pH 1-9 to pH 1-13.

The invention provides a method for measuring a pH value of a sample solution, which is performed by a pH sensing device, including: providing a pH sensing device and a sample solution, wherein the pH sensing device includes a pH sensor array, and the pH sensor array comprises m pH sensors, and m is an integer greater than 1; measuring the sample solution with the pH sensor array for n times, wherein n is an integer greater than 1, n measurement values are generated, each pH sensor generates a measurement value for each measurement, and the total amount of measurement values generated is n×m; and generating a pH value of the sample solution by all of the measurement values generated by the pH sensor array.