The invention discloses a method of collecting undisturbed soil permeability test soil samples by wax sealing. The method comprises the steps that 1, the soil samples are taken out by whole block, and cutting is conducted on soil samples by using a peeling method, the cutting shape is cylindrical, the diameter of each cylindrical soil sample is less than the inner diameter of sampling ring cutter, the height is greater than the height of the sampling ring cutter; 2, wax sealing is conducted on the periphery of the cylindrical soil samples, the soil samples are sealed by wax into cylindrical shapes, the diameter of each cylindrical soil sample is smaller than the inner diameter of the sampling ring cutter; 3, the inner and outer walls of the ring cutter are evenly coated with Vaseline, the wax shape cylindrical soil samples are evenly cut by the ring cutter, complete permeability test soil samples are formed from outside to inside the ring cutter, the Vaseline, the wax seal layer, and the undisturbed soil samples; 4, experiments are conducted by loading the permeability test soil samples into a permeability apparatus; 5, after the experiments are completed, the permeability coefficient calculation is conducted by replacing the cutting ring area with the actual soil sample area. The method of collecting undisturbed soil permeability test soil samples by wax sealing is simple in experiment, the original permeability performance of the undisturbed soil can be guaranteed, and the method is suitably applied to rigid hard plastic clay or strong weathered mudstone.