30 results about "Muscle satellite cell" patented technology

The present invention addresses the problem of providing: a novel composition for suppressing muscular fatty change, which contains a component that can be ingested safely for a long period; and others. It is found that quercetin or a glycoside thereof can suppress the process of the differentiation of a muscle satellite cell into an adipocyte-like cell. In the present invention, quercetin or a glycoside thereof is used in a composition for suppressing muscular fatty change and the like.

The invention discloses a method for separately culturing a human adipose mesenchymal stem cell and a dedicated culture medium thereof. The culture medium used for separately culturing the human adipose mesenchymal stem cell comprises an animal cell basic culture medium, fetal calf serum, an epidermal growth factor and a platelet-derived growth factor. The final concentration of the fetal calf serum is 1-200 ml / l, the final concentration of the epidermal growth factor is 1-100 ng / ml, and the final concentration of the platelet-derived growth factor is 1-100 ng / ml. The adipose mesenchymal stemcell of the invention has CD31-, CD34-, CD45- and HLA-DR-, as well as the phenotype of CD29+, CD44+, CD105+ and Flk-1+. The specificity cell surface marker and the relevant antihelion molecule of a skeletal muscle cell and a vascular endothelia cell can be expressed after inducement is performed in vitro. Muscle fiber, vascular endothelin and functional muscle satellite cells can be differentiated in a muscle injury model mouse body caused by medicine and the expression of dystrophin protein on the ducheme muscular dystrophy (DMD) model mouse (mdx) myolemma can be partially recovered, so as to release the pathological symptom of the model mouse.

The invention relates to an in situ hybridization probe primer, probe and positioning method of Xu's flat scorpion muscle satellite cells, which belong to the field of molecular biology. The primer sequences of the probe template are as SEQ.NO 1 and SEQ.NO 2, and the probe The sequence is shown in SEQ.NO 3. The invention also provides a method for using the probe to carry out in situ hybridization to localize the satellite cells of the flat scorpionfish muscle. The invention locates Xu's flat scorpion muscle satellite cells through the in situ hybridization of skeletal muscle tissue of Pax7 gene, provides theoretical and technical support for exploring the renewal and activation molecular mechanism of muscle satellite cells, and will help to further study Xu's flat Muscle growth and differentiation rules of scorpionfish; in addition, the invention has a certain application prospect in the research of gene positioning and function by in situ hybridization of muscle tissues of other fishes.

Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.

The invention relates to a method for facilitating in vitro multiplication of a bovine skeletal muscle satellite cell. The method comprises the following steps: obtaining a target sequence containing a bovine miR-133b gene sequence; building an expression vector pEGFP-C1-miR133b; transfecting the bovine skeletal muscle satellite cell by the pEGFP-C1-miR133b. By application of the method for facilitating in vitro multiplication of the bovine skeletal muscle satellite cell, the in vitro multiplication ability of the bovine skeletal muscle satellite cell is improved by obtaining over-expression miniature RNA miR-133b of the bovine skeletal muscle satellite cell and using the effect of the miR-133b, and differentiation is effectively inhibited. The over-expression vector of the miR-133b is built by using the pEGFP-C1, and amplification and differentiation of a bovine muscle satellite cell are regulated by the miniature RNA, so as to obtain a high-purity bovine muscle satellite cell. A good cell model is provided for researching the function and the effect of each regulatory factor in amplification and differentiation processes of the muscle satellite cell.

The invention relates to a method for separating and purifying high-purity chicken-precursor intramuscular fat cells and establishing an intramuscular-fat-cell-and-muscle-satellite-cell co-culture system. The method includes the steps that chicken breast muscle tissue is cut into meat paste, and the meat paste is digested with I-type collagenase and centrifuged; upper-layer mature intramuscular fat cells are sucked, inoculated into a culture bottle and subjected to dedifferentiation treatment, and the chicken-precursor intramuscular fat cells are finally obtained; lower-layer cells obtained after centrifuging are precipitated and resuspended, the cells are purified in a differential-wall-attachment mode, and high-purity muscle satellite cells are obtained; the precursor intramuscular fat cells and the muscle satellite cells are respectively inoculated into a transwell chamber or a matched culture plate, and the co-culturing system is established. By means of the method, the technical bottleneck that the chicken-precursor intramuscular fat cells cannot be independently separated and purified all the time is broken through, and the method can be applied to quantitatively simulating muscular tissue, accurately researching the interactional relationship and the molecular regulation mechanism of the chicken intramuscular fat cells and muscle cells in vitro, and screening, researching and developing related nutrients or medicine.

The invention provides an electrical stimulation method as well as relative device and system thereof. The system comprises an output module and a stimulation module, wherein the output module obtains low-frequency and long-term electrical stimulation parameters and transmits the electrical stimulation parameters to the stimulation module; and the stimulation module acts on skeletal muscle according to an electrical stimulation signal produced by the electrical stimulation parameters. With the electrical stimulation method, the system can increase the number and improve the proliferation and the differentiation of muscle satellite cells. A device adopting the system has small size, light weight and convenient carrying and use.

The invention discloses the application of a non-coding small RNA miR-192 in inhibiting the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells. The analog of the non-coding small RNA miR-192 is transfected into sheep The overexpression of intracellular miR‑192 in skeletal muscle satellite cells, compared with the cells overexpressing the control nucleotide sequence, the differentiation of sheep skeletal muscle satellite cells was inhibited, but the proliferation of sheep skeletal muscle satellite cells was inhibited. Capabilities are improved. The present invention also provides the application of miR-192 to inhibit the expression of RB1 protein level in sheep skeletal muscle satellite cells. The present invention discovers for the first time that miR-192 has the function of negatively regulating the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells.