30 results about "Muscle satellite cell" patented technology

The present invention addresses the problem of providing: a novel composition for suppressing muscular fatty change, which contains a component that can be ingested safely for a long period; and others. It is found that quercetin or a glycoside thereof can suppress the process of the differentiation of a muscle satellite cell into an adipocyte-like cell. In the present invention, quercetin or a glycoside thereof is used in a composition for suppressing muscular fatty change and the like.

The invention discloses a method for separately culturing a human adipose mesenchymal stem cell and a dedicated culture medium thereof. The culture medium used for separately culturing the human adipose mesenchymal stem cell comprises an animal cell basic culture medium, fetal calf serum, an epidermal growth factor and a platelet-derived growth factor. The final concentration of the fetal calf serum is 1-200 ml / l, the final concentration of the epidermal growth factor is 1-100 ng / ml, and the final concentration of the platelet-derived growth factor is 1-100 ng / ml. The adipose mesenchymal stemcell of the invention has CD31-, CD34-, CD45- and HLA-DR-, as well as the phenotype of CD29+, CD44+, CD105+ and Flk-1+. The specificity cell surface marker and the relevant antihelion molecule of a skeletal muscle cell and a vascular endothelia cell can be expressed after inducement is performed in vitro. Muscle fiber, vascular endothelin and functional muscle satellite cells can be differentiated in a muscle injury model mouse body caused by medicine and the expression of dystrophin protein on the ducheme muscular dystrophy (DMD) model mouse (mdx) myolemma can be partially recovered, so as to release the pathological symptom of the model mouse.

Cell populations enriched for human myogenic progenitors are obtained by selection on the basis of expression of specific cell surface markers. The muscle progenitor cells are characterized as being CD45−, Mac-1−, GlycophorinA−, CD31− and CD34−, ITGA7hi and CD56 intermediate and methods of use thereof. Methods are provided for the separation and characterization of human myogenic cells, which are precursor cells having the ability to form muscle. The cells are identified and isolated from cells found within the pool of muscle satellite cells, located beneath the basal lamina of mature muscle fibers in the muscle tissue.

The invention relates to an in-situ hybridization probe primer, a probe and a mapping method of the muscle satellite cell of sebastes schlegeli and belongs to the field of molecular biology. A probe template has a primer sequence as shown in SEQ. NO 1 and SEQ. NO 2 and has a probe sequence as shown in SEQ. NO 3. The invention further provides a method for performing in-situ hybridization mapping on the muscle satellite cell of sebastes schlegeli by utilizing the probe. According to the method, the muscle satellite cell of sebastes schlegeli is mapped through skeletal muscle tissue in-situ hybridization of a Pax7 gene, theoretical and technical support can be provided to molecular mechanism study for exploring updating and activating of the muscle satellite cell, and further study on a muscle growth differentiation rule of sebastes schlegeli is favored. Furthermore, the method has a certain application prospect in the aspects of gene mapping and functional study on muscle tissue in-situhybridization of other fishes.

The invention relates to an in situ hybridization probe primer, probe and positioning method of Xu's flat scorpion muscle satellite cells, which belong to the field of molecular biology. The primer sequences of the probe template are as SEQ.NO 1 and SEQ.NO 2, and the probe The sequence is shown in SEQ.NO 3. The invention also provides a method for using the probe to carry out in situ hybridization to localize the satellite cells of the flat scorpionfish muscle. The invention locates Xu's flat scorpion muscle satellite cells through the in situ hybridization of skeletal muscle tissue of Pax7 gene, provides theoretical and technical support for exploring the renewal and activation molecular mechanism of muscle satellite cells, and will help to further study Xu's flat Muscle growth and differentiation rules of scorpionfish; in addition, the invention has a certain application prospect in the research of gene positioning and function by in situ hybridization of muscle tissues of other fishes.

Compositions and methods for modulating HIF-2α to meditate of hypoxia signaling in satellite cells and applications thereof for improving skeletal muscle generation and repair are provided. For example, methods of enhancing, increasing, accelerating or / and otherwise improving skeletal muscle generation or regeneration in a subject in need thereof are disclosed. In some embodiments, the methods include administering the subject an effective amount of HIF-2α inhibitor. The HIF-2α inhibitor can be effective to, for example, increase muscle satellite cell proliferation, differentiation, or a combination thereof in a subject. Composition and methods for improving respiration, and reducing or preventing the development or progression of fibrosis are also provided. The disclosed compositions and methods are particularly useful for treating muscular dystrophies, myopathies, and other muscle-related diseases and disorders.

The invention relates to a method for facilitating in vitro multiplication of a bovine skeletal muscle satellite cell. The method comprises the following steps: obtaining a target sequence containing a bovine miR-133b gene sequence; building an expression vector pEGFP-C1-miR133b; transfecting the bovine skeletal muscle satellite cell by the pEGFP-C1-miR133b. By application of the method for facilitating in vitro multiplication of the bovine skeletal muscle satellite cell, the in vitro multiplication ability of the bovine skeletal muscle satellite cell is improved by obtaining over-expression miniature RNA miR-133b of the bovine skeletal muscle satellite cell and using the effect of the miR-133b, and differentiation is effectively inhibited. The over-expression vector of the miR-133b is built by using the pEGFP-C1, and amplification and differentiation of a bovine muscle satellite cell are regulated by the miniature RNA, so as to obtain a high-purity bovine muscle satellite cell. A good cell model is provided for researching the function and the effect of each regulatory factor in amplification and differentiation processes of the muscle satellite cell.

The invention provides application of grass carp FSRP-3 to a culture medium and further correspondingly provides a special culture medium containing grass carp FSRP-3 and used for grass carp muscle satellite cells. The special culture medium for the grass carp muscle satellite cells, provided by the invention, comprises a basic culture medium and additives, wherein the additives comprise the grass carp FSRP-3, grass carp sourced blood serum extract, sodium pyruvate, glutamic acid and vitamin E. By adopting the special culture medium for the grass carp muscle satellite cells, provided by the invention, the grass carp muscle satellite cells can grow vigorously and a cell culture period is shortened; the special culture medium for the grass carp muscle satellite cells has the characteristics of high efficiency, stability and the like and has a wide market prospect.

The invention relates to a method for separating and purifying high-purity chicken-precursor intramuscular fat cells and establishing an intramuscular-fat-cell-and-muscle-satellite-cell co-culture system. The method includes the steps that chicken breast muscle tissue is cut into meat paste, and the meat paste is digested with I-type collagenase and centrifuged; upper-layer mature intramuscular fat cells are sucked, inoculated into a culture bottle and subjected to dedifferentiation treatment, and the chicken-precursor intramuscular fat cells are finally obtained; lower-layer cells obtained after centrifuging are precipitated and resuspended, the cells are purified in a differential-wall-attachment mode, and high-purity muscle satellite cells are obtained; the precursor intramuscular fat cells and the muscle satellite cells are respectively inoculated into a transwell chamber or a matched culture plate, and the co-culturing system is established. By means of the method, the technical bottleneck that the chicken-precursor intramuscular fat cells cannot be independently separated and purified all the time is broken through, and the method can be applied to quantitatively simulating muscular tissue, accurately researching the interactional relationship and the molecular regulation mechanism of the chicken intramuscular fat cells and muscle cells in vitro, and screening, researching and developing related nutrients or medicine.

The invention provides an electrical stimulation method as well as relative device and system thereof. The system comprises an output module and a stimulation module, wherein the output module obtains low-frequency and long-term electrical stimulation parameters and transmits the electrical stimulation parameters to the stimulation module; and the stimulation module acts on skeletal muscle according to an electrical stimulation signal produced by the electrical stimulation parameters. With the electrical stimulation method, the system can increase the number and improve the proliferation and the differentiation of muscle satellite cells. A device adopting the system has small size, light weight and convenient carrying and use.

The invention discloses the application of a non-coding small RNA miR-192 in inhibiting the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells. The analog of the non-coding small RNA miR-192 is transfected into sheep The overexpression of intracellular miR‑192 in skeletal muscle satellite cells, compared with the cells overexpressing the control nucleotide sequence, the differentiation of sheep skeletal muscle satellite cells was inhibited, but the proliferation of sheep skeletal muscle satellite cells was inhibited. Capabilities are improved. The present invention also provides the application of miR-192 to inhibit the expression of RB1 protein level in sheep skeletal muscle satellite cells. The present invention discovers for the first time that miR-192 has the function of negatively regulating the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells.

The invention discloses an isolated culture method of muscle satellite cells, which comprises the following steps of cutting off leg skin under aseptic conditions, taking leg muscles, and immersing in a buffer solution, removing fat, bones and tendons, and repeatedly cleaning muscle tissues with a buffer solution, shearing muscle tissues into small blocks under a sterile condition, and digesting with collagenase to separate cells, adding an isopyknic culture medium, blowing, beating, filtering, collecting filtrate, centrifuging, discarding supernate, and resuspending cells by using the culture medium, adding the cell suspension into a culture dish, and culturing in a cell incubator, carrying out cell purification culture by a differential attachment method, and when the cell confluence degree reaches 80%, using trypsin for digestion subculture, and when the cell confluence degree reaches 90% or above, carrying out cell cryopreservation. The chicken muscle satellite cells cultured by the isolated culture method disclosed by the invention are higher in activity, more in quantity, easier to adhere to the wall, stronger in passage and higher in differentiation rate, and the culture method is simple, convenient to operate, time-saving and labor-saving.

The invention discloses a porcine akirin2 gene promoter and application thereof, the nucleotide sequence of which is shown in SEQ:ID:1. The application of the pig akirin2 gene promoter of the invention in the eukaryotic expression of exogenous genes and transgenic pigs. The present invention determined that the smallest promoter fragment of 200bp has the strongest activity. The invention provides an oligonucleotide primer sequence, a cloning vector, an expression vector, a transformed host, and a method for exogenous protein expression. These provide the basis for further research on the regulation of porcine akirin2 gene in porcine muscle satellite cells and other related cells, and can be used in mammalian cells for foreign protein expression and transgenic research.

The invention relates to a seawater fish embryonic cell culture technology, in particular to a method for establishing a muscle satellite cell line in the embryonic stage of flounder. The embryos of the flounder bursa formation stage were separated in vitro, the egg membrane was removed, the cells were dispersed, and the embryonic muscle satellite cell line was established after primary culture and subculture. The morphology of the flounder embryo muscle satellite cell line established in the present invention is spindle-shaped or spindle-shaped mononuclear cells. In a single generation, if it is cultured for more than 5 days or induced by exogenous factors (horse serum), it can differentiate and form muscle fibers. The cell line can provide a large number of muscle satellite cells, which can be normally transfected or infected by GFP transfection and semi-smooth tongue sole spleen-kidney necrosis virus challenge experiments. Therefore, it can not only be directly used in the research of functional genes related to fish muscle growth and development, provide research materials for exploring the molecular mechanism of induction, proliferation and differentiation of muscle cells, but also provide research materials for the improvement and application of muscle traits in flounder breeding. platform.