Method for facilitating in vitro multiplication of bovine skeletal muscle satellite cell
A satellite cell and in vitro proliferation technology, applied in the direction of using vectors to introduce foreign genetic material, cells modified by introducing foreign genetic material, recombinant DNA technology, etc., can solve problems such as complex processes and achieve strong in vitro proliferation
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[0025] The present invention will be further described below in conjunction with specific embodiments, and its specific embodiments should be understood as illustrations only, not limiting, and the protection scope of the present invention cannot be limited by the following examples.
[0026] Design idea of the present invention is:
[0027] A pair of PCR primers were designed according to the bovine miR-133b gene sequence, XhoI restriction site and BamHI restriction site were introduced at the 5' ends of the two primers, and miR-133b containing miR-133b was amplified from the bovine genome using the pair of primers The full length of the gene is a sequence of 311bp, and the sequence is inserted into the vector pEGFP-C1 by using the introduced restriction site to construct the expression vector pEGFP-C1-miR133b of the bovine miR-133b gene; then use the obtained expression vector pEGFP- C1-miR133b transfected bovine skeletal muscle satellite cells, so that bovine skeletal mus...
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