A method for promoting myogenic differentiation of bovine skeletal muscle satellite cells by overexpressing psmb5
A satellite cell and myogenic differentiation technology, which is applied in the cell and biological fields, can solve problems such as patent publications that have not yet been found, and achieve the effect of promoting myogenic differentiation ability and improving myogenic differentiation ability
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[0024] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.
[0025] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.
[0026] A method for promoting bovine skeletal muscle satellite cell myogenic differentiation by overexpressing PSMB5, the steps are as follows:
[0027] (1) Acquisition of the target sequence containing the bovine PSMB5 gene sequence:
[0028] Design a pair of PCR primers according to the bovine PSMB5 gene sequence, introduce XhoI restriction site and XbaI restriction site at the 5' ends of the two primers, and use the pair of primers to amplify the CDS region containing the PSMB5 gene from the bovine genome Sequen...
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