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A method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with uch-l3 expression

A technology of UCH-L3 and satellite cells, applied in biochemical equipment and methods, activity regulation, gene therapy, etc., can solve problems such as patent publications that have not been found

Active Publication Date: 2022-05-17
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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  • A method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with uch-l3 expression
  • A method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with uch-l3 expression
  • A method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with uch-l3 expression

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Embodiment Construction

[0021] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0022] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0023] A kind of design train of thought of the present invention is:

[0024] Design and synthesize si-RNA of UCH-L3, then use lip 3000 reagent to transfect bovine skeletal muscle satellite cells to interfere with the expression level of UCH-L3 in bovine skeletal muscle satellite cells, by changing the expression level of UCH-L3 in bovine skeletal muscle satellite cells Expression, thereby inhibiting the myogenic differentiation process of bovine skeletal muscle satellite cells.

[0025] A method for inhibiting the ...

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Abstract

The invention relates to a method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with the expression of UCH-L3, wherein the method is to suppress the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with the expression of UCH-L3, wherein the The genome base sequence of UCH‑L3 is: SEQ NO.1. The method of the present invention regulates the myogenic differentiation process of bovine muscle satellite cells by changing the expression of UCH-L3, which can provide enlightenment for the research on deubiquitinating enzymes of muscle development and differentiation, and provide clinical research, diagnosis and treatment for muscle development and differentiation and injury repair Provide new ideas and references.

Description

technical field [0001] The invention belongs to the technical fields of biology, cell and tissue engineering, in particular to a method for inhibiting bovine skeletal muscle satellite cell myogenic differentiation by interfering with UCH-L3 expression. Background technique [0002] Skeletal muscle satellite cells (skeletal muscle satellite cells) are flat, protruding cells attached to the surface of muscle fibers in skeletal muscle. They usually exist between the sarcolemma and basement membrane in a resting state. Under certain conditions, they can When activated, muscle satellite cells can proliferate, differentiate, and fuse into mature myotubes or muscle fibers. Muscle satellite cells play a very important role in the growth, development and regeneration of muscles in animals after birth. The in vitro myogenic differentiation process of muscle satellite cells well simulates the muscle development process in vivo, and is a good cell model for studying cell differentiation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/86C12N15/113
CPCC12N15/85C12N15/86C12N15/113C12N2310/10C12N2320/50
Inventor 丁向彬胡蕾王轶敏张林林李新郭益文郭宏
Owner TIANJIN AGRICULTURE COLLEGE
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