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Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells

A satellite cell and myogenic differentiation technology, which is applied in the fields of tissue engineering, biology, and cells, can solve problems such as patent publications that have not yet been found, and achieve the effect of improving yield and quality, and improving yield and quality

Active Publication Date: 2020-02-04
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells
  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells
  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells

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Embodiment Construction

[0023] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0024] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0025] A method for promoting the myogenic differentiation of bovine skeletal muscle satellite cells, characterized in that: the method is to promote the myogenic differentiation of bovine skeletal muscle satellite cells by increasing the expression of lncRNA-23, wherein the lncRNA-23 Genome base sequence is: SEQ NO.1.

[0026] Preferably, the specific steps are:

[0027] The overexpression vector pcDNA-lnc23 of lncRNA-23 was constructed, and pcDNA-lnc23 was transfected into bovine skeletal muscle satellite cells to ...

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Abstract

The invention relates to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. The method is characterized in that according to the method, the myogenic differentiation of bovine skeletal muscle satellite cells is promoted by increasing the expression of lncRNA-23, wherein the genome base sequence of the lncRNA-23 is SEQ NO.1. The method can provide inspirationfor researching long non-coding RNA of muscle development differentiation, can lay a good foundation for further researching the action mechanism of key lncRNA in the muscle development process, andprovides inspiration for exploring the muscle growth and development mechanism and improving the yield and quality of beef.

Description

technical field [0001] The invention belongs to the technical field of biology, cell and tissue engineering, in particular to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. Background technique [0002] Skeletal muscle satellite cells (skeletal muscle satellite cells) are myogenic stem cells with differentiation and proliferation potential in skeletal muscle. They usually exist between the sarcolemma and basement membrane of muscle fibers in a resting state, and can be activated under certain conditions. Proliferation and myogenic differentiation to form skeletal muscle cells, muscle satellite cells play a very important role in the growth, development and regeneration of muscles in animals after birth. The in vitro myogenic differentiation process of muscle satellite cells well simulates the muscle development process in vivo, and is a good cell model for studying cell differentiation and muscle development. The activation, proli...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/113C12N5/10
CPCC12N5/0659C12N15/85C12N2510/00
Inventor 郭宏张林林郭益文丁向彬李新陈明明宋瑛燊
Owner TIANJIN AGRICULTURE COLLEGE
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