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Method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression

A technology of UCH-L3 and satellite cells, which is applied in biochemical equipment and methods, gene therapy, DNA/RNA fragments, etc., and can solve problems such as undiscovered patent publications

Active Publication Date: 2020-04-03
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression
  • Method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression
  • Method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression

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Embodiment Construction

[0021] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0022] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0023] A kind of design train of thought of the present invention is:

[0024] Design and synthesize si-RNA of UCH-L3, then use lip 3000 reagent to transfect bovine skeletal muscle satellite cells to interfere with the expression level of UCH-L3 in bovine skeletal muscle satellite cells, by changing the expression level of UCH-L3 in bovine skeletal muscle satellite cells Expression, thereby inhibiting the myogenic differentiation process of bovine skeletal muscle satellite cells.

[0025] A method for inhibiting the ...

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Abstract

The invention relates to a method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression, the myogenic differentiation of bovine skeletal muscle satellite cells is inhibited by interfering UCH-L3 expression, and the genome base sequence of UCH-L3 is SEQ NO.1. The invention also relates to a kit for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression. According to the method, the myogenic differentiation process of bovine muscle satellite cells is regulated and controlled by changing UCH-L3 expression, enlightenment can be provided for deubiquitination enzyme research of muscle development and differentiation, and new thought and reference are provided for clinical researchand diagnosis and treatment of muscle development and differentiation and injury repair.

Description

technical field [0001] The invention belongs to the technical fields of biology, cell and tissue engineering, in particular to a method for inhibiting bovine skeletal muscle satellite cell myogenic differentiation by interfering with UCH-L3 expression. Background technique [0002] Skeletal muscle satellite cells (skeletal muscle satellite cells) are flat, protruding cells attached to the surface of muscle fibers in skeletal muscle. They usually exist between the sarcolemma and basement membrane in a resting state. Under certain conditions, they can When activated, muscle satellite cells can proliferate, differentiate, and fuse into mature myotubes or muscle fibers. Muscle satellite cells play a very important role in the growth, development and regeneration of muscles in animals after birth. The in vitro myogenic differentiation process of muscle satellite cells well simulates the muscle development process in vivo, and is a good cell model for studying cell differentiation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/86C12N15/113
CPCC12N15/85C12N15/86C12N15/113C12N2310/10C12N2320/50
Inventor 丁向彬胡蕾王轶敏张林林李新郭益文郭宏
Owner TIANJIN AGRICULTURE COLLEGE
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