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Freshwater fish resting state muscle satellite cell stabilizer

A muscle satellite cell and freshwater fish technology, applied in DNA/RNA fragments, recombinant DNA technology, animal husbandry, etc., can solve the problem of inability to maintain the resting level of muscle satellite cells from the source, difficulty in maintaining stability of muscle satellite cells, and cumbersome treatment and other issues, to achieve the effect of easy operability of core technology, broad market prospects, and advanced core technology

Active Publication Date: 2016-03-02
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current in vivo and in vitro experiments have shown that muscle satellite cells in the resting state of fish are difficult to maintain stability, and knocking out myogenic regulators can inhibit the activation and differentiation of muscle satellite cells. Maintain resting levels of muscle satellite cells

Method used

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  • Freshwater fish resting state muscle satellite cell stabilizer
  • Freshwater fish resting state muscle satellite cell stabilizer
  • Freshwater fish resting state muscle satellite cell stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The influence of embodiment 1 stabilizer on the expression of mandarin fish muscle serum response factor

[0021] The role of stabilizers in reducing serum response factors in muscles was studied by intramuscular injection of mandarin fish:

[0022] The negative control group was not injected with any reagent, the positive control group was injected with phosphate buffered saline (PBS), and the test group was injected with stabilizers. The injection dose of the test group was 20 μg / g body weight, injected for three consecutive days, once a day, One week after the injection, the back muscles of mandarin fish were taken to quantitatively analyze the expression of serum response factors. The specific results are as follows: figure 1 shown.

[0023] Depend on figure 1 It can be seen that the expression of mandarin fish serum response factors in the test group injected with stabilizers was significantly lower than that of the positive and negative control groups; the diffe...

Embodiment 2

[0024] Example 2 Effect of Stabilizers on Grass Carp Muscle Serum Response Factor Expression

[0025] By intramuscular injection of grass carp, the effect of stabilizers on reducing serum response factors in muscle was studied:

[0026] The experimental grouping is as in Example 1. After one week of the experiment, the grass carp back muscles were taken to quantitatively analyze the expression of serum response factors. The specific results are as follows: figure 2 shown.

[0027] Depend on figure 2 It can be seen that the expression of serum response factors of grass carp muscle in the test group injected with stabilizer was significantly lower than that of the positive control group and the negative control group; the difference in the expression of serum response factors of grass carp in the positive control group and the negative control group was not significant; therefore, it can be judged that the injection stability The agent can reduce the expression of serum resp...

Embodiment 3

[0028] The effect of embodiment 3 stabilizer on grass carp resting state muscle satellite cell quantity

[0029] The experimental grouping is as in Example 2. After one week of the experiment, the back muscles of grass carp were taken for index measurement. The specific results are as follows: image 3 shown.

[0030] Depend on image 3 It can be seen that compared with the positive control group and the negative control group, the number of Pax7-positive muscle satellite cells in the test group injected with stabilizers was significantly higher than that of the positive control group and the negative control group; the Pax7-positive muscle satellite cells in the positive control group and the negative control group Quantitative differences were not significant. Therefore, it can be judged that injection of stabilizer can maintain the quiescent state of grass carp muscle satellite cells.

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Abstract

The invention discloses a freshwater fish resting state muscle satellite cell stabilizer which is prepared from double-strand small RNA molecules which are marked, chemically modified and chemically synthesized. The forward sequence of the double-strand small RNA molecules is 5'-AAAUGUAGUAGACUAUAAGUAU-3'. The reverse sequence of the double-strand small RNA molecules is 5'-AsUsACUUAUAGUCUACUACAsUsUsUs-Chol3'. The reverse sequence is used for chemical modification. The 3' end is provided with a cholesterol modifier. The 5' end is provided with two sulpho-modifiers, and the 3' end is provided with four sulpho-modifiers. The reverse sequencec is used for full-base methylation modification. A novel small RNA compound keeping the freshwater fish muscle satellite cell resting state is artificially synthesized. The prepared stabilizer can obviously inhibit the content of main protein promoting muscle satellite cell activation, and the resting state of freshwater fish muscle satellite cells is kept.

Description

technical field [0001] The invention relates to the technical field of biological genes, in particular to a freshwater fish resting muscle satellite cell stabilizer. Background technique [0002] The resting muscle satellite cells are located between the sarcolemma and the basement membrane. When stimulated by an activation signal, the muscle satellite cells are activated, proliferate, and fuse with the original skeletal muscle cells to form new muscle fiber cells. Quiescent muscle satellite cells (QMSCs) are morphologically characterized by less cytoplasm, loose organelles, similar size to adjacent muscle fiber nuclei, high ratio of heterochromatin to euchromatin, and low transcriptional activity. Under the action of a variety of stimuli, the cytoplasm of muscle satellite cells in the resting state in vivo expands and increases in volume, and when organelles such as Golgi apparatus, endoplasmic reticulum, ribosomes, and mitochondria become clear and obvious, it indicates th...

Claims

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Application Information

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IPC IPC(8): C12N15/113A01K67/027
Inventor 褚武英唐利芝胡博旸李玉珑
Owner HUNAN AGRICULTURAL UNIV
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