Application of ubiquitin-specific protease 8 in regulation and breeding of Hu mutton
A specific, protease technology, applied in the application of ubiquitin-specific protease 8 in the regulation of Hu mutton meat quality and breeding, can solve the problem of no USP8 regulating muscle related reports and other issues
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[0036] 1 Test materials and methods
[0037] 1 Test materials and equipment
[0038] 1.1 Test material
[0039] The six-month-old male Hu sheep (Ovis aries) used in this experiment came from Zhongcheng Hu Sheep Professional Cooperative in Tongxiang, Jiaxing City, Zhejiang Province. The average weight of the Hu sheep was 48.50±2.95kg. Hu sheep were fasted 24 hours before sampling. The F1 generation of six-month-old male Duhu hybrid sheep used in this experiment came from Lin'an sheep farm in Hangzhou City, Zhejiang Province, and also fasted for 24 hours before sampling.
[0040] The Huyang muscle satellite cells used in this experiment were donated by the research group of Professor Sun Wei of Yangzhou University.
[0041] 1.2 Main chemical reagents
[0042] Absolute ethanol (Shanghai Hushi, Sinopharm Chemical Reagent Co., Ltd.); DEPC treated water, 1×PBS buffer solution (Shanghai Sangon Biotechnology Co., Ltd.); xylene (Sinopharm Chemical Reagent Co., Ltd.); masson dye sol...
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