30 results about "Ubiquitin specific protease" patented technology

The present invention relates to quinazolin-4-one compounds of formula (I′), their process of preparation and uses thereof. These compounds are useful as selective and reversible inhibitors of ubiquitin specific proteases, particularly USP7, for treating e.g. cancer, neurodegenerative diseases, inflammatory disorders and viral infections.

The invention discloses a function and application of ubiquitin specific protease 4 (USP4) in treating cardiac hypertrophy, and belongs to the field of gene functions and application. The mutual relation between the expression of the USP4 and cardiac hypertrophy is determined; research results show that in a cardiac hypertrophy occurrence model, the expression of the USP4 is significantly reduced compared with a normal group; by restraining the expression of the USP4, the cardiac hypertrophy and fibrosis are significantly promoted, and the cardiac function is deteriorated; by promoting the expression of the USP4, cardiac hypertrophy and fibrosis are significantly restrained, and the cardiac function is protected. Accordingly, the USP4 can be used as a target gene for screening and preparing the medicine which protects the cardiac function, resists cardiac fibrosis and / or prevents, relieves and / or treats cardiac hypertrophy, and an effective new way is provided for treating cardiac hypertrophy.

Disclosed are small molecule inhibitors of deubiquitinating enzymes (DUBs), and methods of using them. Certain compounds display a preference for specific ubiquitin specific proteases (USPs).

The present invention generally relates to 6-amino-2,4-dihydropyrano [2,3-c] pyrazoles as a ubiquitin specific protease 7 (USP7) inhibitor useful for the treatment of diseases mediated by malfunction of USP7, such as inflammation, cancer, and immunological disorders. The invention described herein also pertains to pharmaceutical compositions and methods for treating diseases mediated by malfunction of USP7, in mammals using compounds disclosed herein.

The invention provides a recombinant lentiviral vector containing ubiquitin-specific protease gene USP39-shRNA (short hairpin ribonucleic acid) and application thereof. The test proves that expression of USP39 in breast cancer tissue is obviously higher than that in normal breast tissue; the immunohistochemical detection confirms that the expression of USP39 in the breast cancer tissue is obviously higher than that in normal breast tissue; the immunohistochemical detection confirms that expression of USP39 protein in the breast cancer tissue is obviously higher than that in para-carcinoma tissue; siRNA (small interfering ribonucleic acid) is designed in a breast cancer cell system to interfere the expression of the USP39; it is found that proliferation of breast cancer cell after reducing the USP39 is obviously restrained; the cell apoptosis ratio is obviously improved; the cell ratio at the G1 stage is increased; the cell ratio at the S sage is reduced; and the clonality of the cell is obviously reduced. The test proves that the USP39 has an important role in facilitation of development of the breast cancer; and theoretical and experimental basis is provided for preparation of a drug for preventing and treating the breast cancer by the lentiviral vector containing USP39-shRNA.

The invention discloses hydroxy substituted tetrahydro-beta-carboline small-molecular organic compounds and derivatives, hydrates or pharmaceutically acceptable salts of the compounds, pharmaceuticalcomposition containing the compounds, an application of the compounds to preparation of drugs for preventing and / or treating various USP (ubiquitin-specific protease)15-mediated malignant tumors and tumor metastasis related diseases.

The invention provides a pharmaceutical composition containing a USP13 inhibitor and a PARP inhibitor and application thereof. Specifically, the invention provides a pharmaceutical composition which contains the active constituent (a) USP13 inhibitor, the active constituent (b) PARP inhibitor, and (c) a pharmaceutically acceptable carrier. The USP13 inhibitor can remarkably improve the anti-tumor effect of the PARP inhibitor, and the homologous recombination repair efficiency of tumor cells can be reduced remarkably through combination of the two inhibitors, so that tumor growth can be suppressed more effectively. The pharmaceutical composition can effectively treat cancer.

A method of identifying a lead or candidate compound that modulates the activity of GTPase-Activating Protein SH3 Domain-Binding Proteins (G3BP) is provided, which includes determining whether a compound modulates the interaction between the N-terminal Nuclear Transport Factor 2-like (NTF2L) domain of G3BP and FGDF peptide of ubiquitin specific protease 10 (USP10) or non-structural protein 3 (nsP3).

A ubiquitin-specific protease occurring in the brain and a DNA encoding it, which are useful for research on the molecular mechanism of the neuroplasticity expression and so on.

The invention provides application of ubiquitin-specific proteases USP7 to preparing drugs for treating aging and related diseases. The amino acid sequence of the USP7 is shown as SEQ. NO.2. Accordingto the application of the ubiquitin-specific proteases USP7 to preparing the drugs for treating aging and related diseases, the USP7 is activated to avoiding degradation of telomere proteins and damage of telomeres, particularly degradation of telomere proteins in environmental stress states such as radiation damage or oxidative stress., and meanwhile, avoid telomere damage and shortening, tissueand organ aging and related diseases caused by pressurized or stressed anaerobic states; expression and enzyme activity of the USP7 is inhibited to prevent protecting effects of the USP7 on the telomere proteins and the telomeres, to lead to telomere shortening and damage response, and further to cause proliferation and growth inhibition, aging and death of tumor cells and inhibit generation, development, growth, infiltration and metastasis of tumor. The USP7 can be applied to preparing drugs for preventing and treating telomere function related diseases and provide new anti-aging thoughts and methods.

The invention relates to a cholangiocarcinoma cell medicine target point. The medicine target point is ubiquitin specific protease 8, and the nucleotide sequence is shown as SEQID NO:1 in a sequence table. For the first time, the invention puts forward that USP8 is knocked down, so that proliferation, migration and invasion of cholangiocarcinoma cells can be notably restrained, MMP9 activity achieving important effects on cell adhesion, neoplasm invasiveness and transfer is reduced, and the USP8 is promoted to achieve effect of oncogene for growth and transfer ability of cholangiocarcinoma. New possibility is provided for development of cholangiocarcinoma medicines and cholangiocarcinoma prevention, treatment and prognosis.

The invention relates to the application of ubiquitin-specific protease 8 (USP8) in regulating the meat quality and breeding of Hu mutton. Experiments have found that USP8 can affect the eating quality of Hu sheep muscle. The higher the expression level of USP8, the better the eating quality of Hu sheep. Therefore, Hu sheep with good meat quality can be selected as breeding sheep by screening the expression level of USP8. The invention clarifies that USP8 has an inhibitory effect on the early differentiation process of muscle satellite cells, provides a basic theoretical basis for the further protection and breeding of Hu sheep breeds, and also provides some references and technical reserves for the future directional selection of new strains .

Provided are USP19 inhibitors, methods of treating obesity, metabolic syndrome and / or diabetes using the USP19 inhibitor compounds, as well as those compounds for use in methods of treating obesity, metabolic syndrome and / or diabetes. Also provided are methods of treating muscular atrophy, for example cachexia or sarcopenia with USP19 inhibitor compounds, plus those a compounds for use in methods of treating muscular atrophy.

The present invention relates to USP47 (ubiquitin specific protease 47) inhibitors and methods for inducing apoptosis or cell death in a target cell. In certain embodiments, the invention relates to methods and kits to screen for related agents that induce apoptosis. Additionally, the invention relates to assays for screening compounds capable of acting as USP47 inhibitors.

Resistance of acute myeloid leukemia (AML) cells to DNA damaging therapeutic agents is dependent on CHK1 protein levels. Here, the inventors demonstrate that in AML, CHK1 protein stability relies on the expression and activity of Ubiquitin Specific Protease 7 (USP7). CHK1 and USP7 levels are positively correlated in AML cell lines and primary patient specimens with high CHK1 protein levels. USP7 associates with CHK1, leading to its stabilization by deubiquitinylation, and this association is enhanced in response to cytarabine treatment. Pharmacological or RNA interference-mediated inhibition of USP7 significantly reduced AML proliferation in vitro and in vivo, and increased AML cell death. It is important to note that USP7 inhibition synergized with cytarabine to kill AML cell lines. This is also the case in primary patient specimens with high CHK1 levels. Transcriptomic dataset analyses revealed that a USP7 gene signature is highly enriched in cells from AML patients at relapse, as well as in residual blasts from Patient Derived Xenograft (PDX) models treated with clinically relevant doses of cytarabine, strongly suggesting a relationship between USP7 expression and resistance to therapy. Finally, single cell analysis from AML patient at relapse versus diagnosis showed that a gene signature of the pre-existing subpopulation responsible for relapse is enriched in transcriptomes of patients with high USP7 level. Altogether, these data demonstrate that USP7 is a master regulator of CHK1 protein kinase in AML cells, and represents both a marker of resistance to chemotherapeutic treatments, as well as a potential therapeutic target to overcome treatment resistance.

The invention discloses a quinazolinone USP7 inhibitor, which has a structure shown in a general formula I, has a good inhibition effect on ubiquitin-specific protease 7, can be used for preparing a medicine for inhibiting the ubiquitin-specific protease 7, and shows good inhibition activity on a gastric cancer cell line, especially an MGC803 cell line. A lead compound structure is provided for anti-cancer drugs, and the compound has a good application prospect. The invention further provides a preparation method of the quinazolinone USP7 inhibitor. The preparation method is simple, mild in reaction condition, few in by-product, high in reaction yield and convenient for batch production and commercial application.

Inhibitors of ubiquitin specific protease 19 (USP19) of Formula (I) are provided, together with pharmaceutical compositions comprising said inhibitors, and methods of use thereof. The compounds can be used in in the treatment of muscular atrophy, obesity, insulin resistance or type II diabetes or in reducing the loss of muscle mass.

The invention discloses an application of a USP7 inhibitor in a medicine for treating ABC-DLBCL. The expression condition of the USP7 in a DLBCL cell line is studied; through database analysis, a USP7small-molecule inhibitor is used for inhibiting USP7 activity; the influence of ABC type DLBCL cell proliferation, PI staining and flow cytometry on cell apoptosis and the influence of cell apoptosison an ABC type DLBCL BCR signal path by a CCK-8 method can be observed; USP7 is a ubiquitin specific protease which is most widely researched at present; researches show that USP7 participates in regulating the activity and functions of various protein substrates of cells, USP7 expression abnormality or activity change plays an important role in occurrence and development of diseases and tumors,and USP7 has good application prospects and market competitiveness and has a good promotion effect on improvement of people's livelihood and society in China.

Compositions and methods for treating cancer are provided. In accordance with the instant invention, methods of inhibiting or treating cancer, particularly a “cold” cancer or a cancer lacking CD8+ tumor infiltrating lymphocytes (TILs) and / or other immunostimulatory features, in a subject are provided. In a particular embodiment, the method comprises administering an agent which increases ubiquitin-specific protease 6 (USP6) activity and / or expression to the subject.