Recombinant lentiviral vector containing ubiquitin-specific protease gene USP39-shRNA (short hairpin ribonucleic acid) and application thereof

Abstract

The invention provides a recombinant lentiviral vector containing ubiquitin-specific protease gene USP39-shRNA (short hairpin ribonucleic acid) and application thereof. The test proves that expression of USP39 in breast cancer tissue is obviously higher than that in normal breast tissue; the immunohistochemical detection confirms that the expression of USP39 in the breast cancer tissue is obviously higher than that in normal breast tissue; the immunohistochemical detection confirms that expression of USP39 protein in the breast cancer tissue is obviously higher than that in para-carcinoma tissue; siRNA (small interfering ribonucleic acid) is designed in a breast cancer cell system to interfere the expression of the USP39; it is found that proliferation of breast cancer cell after reducing the USP39 is obviously restrained; the cell apoptosis ratio is obviously improved; the cell ratio at the G1 stage is increased; the cell ratio at the S sage is reduced; and the clonality of the cell is obviously reduced. The test proves that the USP39 has an important role in facilitation of development of the breast cancer; and theoretical and experimental basis is provided for preparation of a drug for preventing and treating the breast cancer by the lentiviral vector containing USP39-shRNA.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to recombinant lentivirus containing human ubiquitin-specific protease gene USP39-shRNA and application thereof. Background technique [0002] The ubiquitin-specific proteases (USPs) are the main members of the deubiquitinase family, which can recognize the specific sequence of ubiquitin and substrate, destroy the link between substrate protein and ubiquitin chain, play a role in deubiquitination. Deubiquitinases are involved in the regulation of cell cycle progression, the regulation of cell signal transduction, and the growth and development of organisms. More and more studies have found that the abnormal expression of USP family is closely related to various pathological changes, such as the occurrence of various tumors. Although more than 60 members of the USP family have been found in the human genome, the functions and mechanisms of most USPs are still unclear. [0003] The...

AI Technical Summary

Problems solved by technology

[0004] Through the analysis of these existing studies, it is found that the function of USP39 is far from elucidated, and how it participates in the occurrence and development of breast cancer has not been studied so far.

Images