Recombinant lentiviral vector containing ubiquitin-specific protease gene USP39-shRNA (short hairpin ribonucleic acid) and application thereof
A technology of recombinant lentivirus and usp39-shrna, which is applied in the field of molecular biology, can solve the problems of breast cancer that have not been studied and the function of USP39 has not been elucidated.
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[0032] The RNA extraction, reverse transcription and fluorescent quantitative reagents used in the present invention were purchased from Bao Bioengineering (Dalian) Co., Ltd., antibodies were purchased from Abcom, and various cell lines were purchased from ATCC (American Type Microorganism Collection Center) and Shanghai Cell Bank of Chinese Academy of Sciences (http: / / www.cellbank.org.cn / mulu.asp), various restriction endonucleases were purchased from NEB Company, the plasmids and gene chips used were purchased from Shanghai Jikai Biotechnology Company, and the plasmid extraction kit was purchased from Omega Company, Plus-20 centrifugal ultrafiltration device was purchased from Millipore Company, various biochemical reagents were purchased from Sigma Company, agarose was purchased from HydraGene Company, Alexa Fluor488annexin V / Dead Cell Apoptosis Kit, lipofectamine2000 were purchased from Invitrogen Company, ECL luminescence substrate The detection kit was purchased from Mill...
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