77 results about "Insect virus" patented technology

Compositions and methods for reducing susceptibility to infectious disease in bees using RNA interference technology, and more particularly, prevention and treatment of viral infections in honeybees such as Israel acute paralysis virus (IAPV) by feeding of pathogen-specific dsRNA. Further, multiple-pathogen specific dsRNA is disclosed.

It is intended to provide a protein complex and a production process whereby the protein complex can be efficiently produced without lowering its function. It is also intended to provide use of the protein complex in a biosensor, an immobilized enzyme and so on. A protein complex comprising a polyhedral protein having an insect virus encapsulated therein and a target protein having a restricted region of a capsid-protein VP3 of cytoplasmic polyhedrosis virus, more specifically, a region which is either a region from the N-terminus to the 40th amino acid residue or a region from the 41st amino acid residue to the 79th amino acid residue as an embedding signal for polyhedron, and a process for producing the same. The polyhedral protein has an effect on improvement in the stability of the target protein, protection thereof or improvement in the preservation properties thereof, or a combination of any of these. The target protein is at least one member selected from the group consisting of fluorescent or light-emitting proteins, enzymes, antigens, antibodies, cytokines, receptors and bioactive proteins. A biosensor characterized in that the above-described protein complex is arranged in dots or lines on a substrate and immobilized thereon.

Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.

The present invention discloses the synergistic method for nucleopolyhedron virus. For sake of overcoming the demerits of single insect virus, such as slow pesticidal speed, low toxicity, etc, insect growth regulator chlorfluazuron is made to cooperate with insect virus so as to raise the pest controlling effect obviously. Compared with the traditional method of raising the pest controlling effect of virus with neurotoxic pesticide, the present invention has the advantages of high synergistic effect, high safety and raised biological control level of pests.

A method for controlling the Prodenia litura features that the separated different strains of a same nuclear polyhedron virus are combined for high insecticiding speed and efficiency. Its advantages are high effect and high safety.

The invention discloses a genetic engineering protein of coronavirus and its application. That is genetic engineering protein FSPA relevant to S protein of coronavirus SARS- CoV and nucleotide sequences amnio sequence and code said FSPA. The invention also discloses a recombinant insect virus strain containing SARS- CoV Spike- recombinant autographa californica nucleopolyhe-drovirus AcNPV- FSPA, CCTCC No.V200513, inserts into the expression box of SARS- CoV Spike. The inventiton also discloses the application of FSPA in checking the coronavirus SARS- CoV, which is the pathogen for acute respiratory syndrome.

The invention relates to a preparation of an ultraviolet-resistant inactivating insect virus and belongs to the field of green agricultural biological prevention and control. The invention aims to solve the technical problems that the present insect virus is easy to inactivate at outside and poor in stability. The preparation is realized according to the following method: 1) preparing chitosan-virus mixed solution; 2) preparing an oil phase; and 3) biologically preparing a chitosan microcapsule. The ultraviolet-resistant inactivating insect virus according to the invention is capable of reducing the environmental pollution, promoting the use stability and reducing the production cost, and meanwhile, is capable of effectively prolonging the pesticide effect duration of the insect virus andis applicable to the fields of control on crop growth, crop pest killing, and the like.

The invention is an application of cnidiadin as insect virus synergist to the biologic prevention and cure. Cnidiadin derives from common cnidium fruit extract, when used singly, has not pesticidal activity, but when associated with insect nuclear polyhedrosis virus (AcNPV, SINPV, MbNPV, etc.) and granulosis virus (XcGV, etc.), can remarkably enhance the infection capability of insect virus to pest. The dosage is low (1-228mg / L), and the concentration lower, the synergistic action stronger, safe to entironment.

A process for increasing the reproduction efficiency of insect virus is characterized by that the synergistic protein of insect virus and the inclusion body of insect virus are exploded to obtain virus particles, which are used to infect the adult insects for high-efficiency and high-level reproduction of said virus in the insects.

The invention discloses a method for synergizing the beet armyworm nuclear polyhedrosis virus. Aiming at the defects of slow insecticidal speed and low toxicity of a single natural insect virus, the joint action of insect larvae parasitoids and viruses is used to treat the beet armyworm at the same time with the beet armyworm nucleopolyhedrosis virus, or parasitize first Bee treatment and virus treatment of beet armyworm can significantly increase the infectivity of the virus to pests. Compared with the traditional use of neurotoxic chemical pesticides to increase the effect of virus control, this technology uses the joint action of the larvae parasitic wasp scutellaria and the virus, which not only has outstanding synergistic effect, but also has high safety, which can effectively improve the biological control of pests. Level. After a large number of applications in production, the economic, social and ecological benefits will be very significant.