77 results about "Insect virus" patented technology

Compositions and methods for reducing susceptibility to infectious disease in bees using RNA interference technology, and more particularly, prevention and treatment of viral infections in honeybees such as Israel acute paralysis virus (IAPV) by feeding of pathogen-specific dsRNA. Further, multiple-pathogen specific dsRNA is disclosed.

Compositions and methods for reducing susceptibility to infectious disease in bees using RNA interference technology, and more particularly, prevention and treatment of viral infections in honeybees such as Israel acute paralysis virus (IAPV) by feeding of pathogen-specific dsRNA. Further, multiple-pathogen specific dsRNA is disclosed.

The present invention discloses a human interleukin-12 recombinant insect virus strain, the recombinant Autographa california multiple nuclear polyhedrosis virus AcNPV-hIL12 virus strain, CCTCC No.2 V200108 and its preparation. The recombinant virus strain is inserted into human interleukin-12 expressing box, and the expressing box includes double promoter sequence, P35 subunit DNA encoding sequence of human interleukin-12 and P40 subunit DNA encoding sequence. P35 subunit gene locates in the downstream of Polyhederin and P40 subunit gene locates in the downstream of P10 promoter. The present invention can express human interleukin-12, and the cell factor is safe and reliable to human body and suitable for treating tumor bacteria, virus and various infections diseases.

It is intended to provide a protein complex and a production process whereby the protein complex can be efficiently produced without lowering its function. It is also intended to provide use of the protein complex in a biosensor, an immobilized enzyme and so on. A protein complex comprising a polyhedral protein having an insect virus encapsulated therein and a target protein having a restricted region of a capsid-protein VP3 of cytoplasmic polyhedrosis virus, more specifically, a region which is either a region from the N-terminus to the 40th amino acid residue or a region from the 41st amino acid residue to the 79th amino acid residue as an embedding signal for polyhedron, and a process for producing the same. The polyhedral protein has an effect on improvement in the stability of the target protein, protection thereof or improvement in the preservation properties thereof, or a combination of any of these. The target protein is at least one member selected from the group consisting of fluorescent or light-emitting proteins, enzymes, antigens, antibodies, cytokines, receptors and bioactive proteins. A biosensor characterized in that the above-described protein complex is arranged in dots or lines on a substrate and immobilized thereon.

A collecting method in insect viruses breed includes: 1) feeding three-year old larva by 500,000 polyhedrons / ml concentration for 3 days, 2) primary collecting larva after experimenting 7-8 days under 24deg.C environment,3) extracting virus and processing viral imagocide after reserving 20-30 days under 15deg.C homeothermal condition. It achieves high insect virus output, simple viral collecting process, electrical save.

The invention is an application of cnidiadin as insect virus synergist to the biologic prevention and cure. Cnidiadin derives from common cnidium fruit extract, when used singly, has not pesticidal activity, but when associated with insect nuclear polyhedrosis virus (AcNPV, SINPV, MbNPV, etc.) and granulosis virus (XcGV, etc.), can remarkably enhance the infection capability of insect virus to pest. The dosage is low (1-228mg / L), and the concentration lower, the synergistic action stronger, safe to entironment.

Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.

The present invention discloses the synergistic method for nucleopolyhedron virus. For sake of overcoming the demerits of single insect virus, such as slow pesticidal speed, low toxicity, etc, insect growth regulator chlorfluazuron is made to cooperate with insect virus so as to raise the pest controlling effect obviously. Compared with the traditional method of raising the pest controlling effect of virus with neurotoxic pesticide, the present invention has the advantages of high synergistic effect, high safety and raised biological control level of pests.

The invention discloses a virus insecticidal suspension agent containing an insect hormone insecticide. The virus insecticidal suspension agent comprises the following components in percentage by weight: 0.1-30% of the insect hormone insecticide, 1-10% of a dispersant, 1-7% of an antifreezing agent, 0.2-10% of a stabilizer, 0.1-5% of a synergist, 0.5-2% of an ultraviolet protecting agent, 0.1-2% of a defoaming agent, 0.1-3% of a pH regulator and the balance of water, wherein the prepared suspension agent also contains an insect virus which is one of or a combination of a nuclear polyhedrosis virus (NPV) and granulosis virus, and the content of an insect virus inclusion body in the suspension agent is 0.5-20 billion PIB / ml. The insect hormone insecticide and a virus insecticide of the virus insecticidal suspension agent disclosed by the invention are subjected to complex pairing, so that the problem of insect resistance can be solved, and the virus insecticidal suspension agent is safe and environmentally friendly.

The invention discloses a method for realizing efficient covering of a foreign protein by using an insect virus polyhedron, wherein the polyhedrin of a bombyx mori cytoplasmic polyhedrosis virus (BmCPV) and a target protein gene are constructed under the PH and P10 promoters on a pFastBac Dual carrier, and a baculovirus capable of expressing the polyhedron and the target protein simultaneously can be obtained through recombination. The method is characterized in that the cytoplasmic polyhedrosis protein gene and the target protein gene are simultaneously constructed under the PH and P10 promoters on the pFastBac TMDual carrier through a baculovirus expression system, respectively, a flexible link peptide is introduced between the target protein and a signal peptide, and the recombinant baculovirus is obtained through recombination with a virus skeleton. Once Sf9 or Sf21 insect cells are infected by the single virtus, the desired covered protein can be directly generated in cells; the recombinant virtus also can be amplified.

The invention discloses viral insecticide containing dihalo pyrazole amide. The viral insecticide consists of dihalo pyrazole amide, adjuvants and insect viruses, wherein the adjuvants comprise a carrier, a dispersant, an antifreezer, an antifoamer, a humectant and an ultraviolet protecting agent. According to the viral insecticide disclosed by the invention, dihalo pyrazole amide and the insect viruses serve as effective ingredients and are compounded, so that the insecticidal spectrum is enlarged, the synergistic effect is obvious, the pest killing speed is increased, the insecticidal duration is prolonged, the usage amount of the effective ingredients is reduced, the safety is enhanced, the generation of insecticide resistance of pests is retarded, and thus, the viral insecticide can be extensively applied to the field of agriculture.

The invention discloses a method for splitting an insect body by utilizing an insect virus to extract insect peptide. A living insect body is fed with the corresponding insect virus; the insect virus is greatly duplicated in the insect body, and a great number of proteolytic enzymes are generated; the structural protein of the insect body can be hydrolyzed into water-soluble peptide by the proteolytic enzymes, so that the insect body is hydrated; after an insect dies from hydration, a liquid mixture A containing an insect skin which is not hydrolyzed by the proteolytic enzymes, the insect virus and the water-soluble peptide is obtained; the insect skin in the liquid mixture A is filtered by a screen, and a liquid mixture B is obtained; the liquid mixture B is subjected to centrifugal treatment; a water-soluble peptide solution positioned on an upper centrifugal layer is separated. The water-soluble peptide solution which is obtained through the method and comes from the insect body is rich in the water-soluble peptide, and has a remarkable effect when serving as a plant foliar fertilizer; in addition, the process of the method is simple, the conditions are mild, and the method is economical, environmentally-friendly and efficient.

A method for controlling the Prodenia litura features that the separated different strains of a same nuclear polyhedron virus are combined for high insecticiding speed and efficiency. Its advantages are high effect and high safety.

The invention discloses a virus pesticide suspending agent containing spinosad. The virus pesticide suspending agent is prepared from the following components in percentage by weight: 0.1%-20% of spinosad, 1%-10% of a dispersing agent, 1%-7% of an antifreezing agent, 0.2%-10% of a stabilizer, 0.1%-5% of a synergist, 0.5%-2% of an ultraviolet protecting agent, 0.1%-2% of an antifoaming agent, 0.1%-3% of a pH modifier and the balance of water; the prepared suspending agent also absorbs an insect virus; the insect virus is one or combination of two of a nuclear polyhedrosis virus and a granulosis virus NPV; the content of an insect virus inclusion body in the suspending agent is 5-20,000,000,000 PIB / ml; and the spinosad is one or a mixture of two of spinosad and ethyl spinosad. The spinosad and a virus pesticide disclosed by the invention are compounded, so that the problem of insect resistance can be solved, and the virus pesticide suspending agent is environment-friendly and safe.

The invention discloses a genetic engineering protein of coronavirus and its application. That is genetic engineering protein FSPA relevant to S protein of coronavirus SARS- CoV and nucleotide sequences amnio sequence and code said FSPA. The invention also discloses a recombinant insect virus strain containing SARS- CoV Spike- recombinant autographa californica nucleopolyhe-drovirus AcNPV- FSPA, CCTCC No.V200513, inserts into the expression box of SARS- CoV Spike. The inventiton also discloses the application of FSPA in checking the coronavirus SARS- CoV, which is the pathogen for acute respiratory syndrome.

An indoxacarb contained virus insecticide suspension agent comprises components in percentage by weight as follows: 0.1%-10% of indoxacarb, 1%-10% of a dispersant, 1%-7% of an antifreezing agent, 0.2%-10% of a stabilizing agent, 0.1%-5% of a synergist, 0.5%-2% of an ultraviolet protection agent, 0.1%-2% of a defoaming agent, 0.1%-3% of a PH regulator and the balance of water. Insect viruses are contained in the well prepared suspension agent and are one or combination of an NPV (nuclear polyhedrosis virus) and a granulosis virus, and the content of an insect virus inclusion body in the suspension agent is 0.5-20 billion PIB / ml. The pest resistance problem can be solved through compounding of indoxacarb and the virus insecticide, and the suspension agent is safe and environment-friendly.

The invention relates to the technical field of biopesticides, in particular to a sodium alginate / milk fat powder insect virus microcapsule and a preparation method thereof. The preparation method ofthe microcapsule comprises the following steps: 1, adding insect virus dry powder into whey protein-containing milk fat powder-water mixed liquid, and uniformly stirring to form a mixture A; 2, addingthe mixture A into a sodium alginate solution, and uniformly stirring to form a mixture B; 3, atomizing the mixture B, spraying the atomized mixture B into a calcium chloride solution, and stirring to obtain a mixture C; 4, filtering the mixture C, taking filter residues, washing the filter residues with deionized water, and collecting wet microcapsules; and 5, pre-freezing the wet microcapsulesat (-90)-(-60) DEG C, then taking out the pre-frozen wet microcapsules, and carrying out freeze drying treatment at (-80)-(-40) DEG C. The microcapsule provided by the invention can be applied to control of prodenia litura, beet armyworm and other insects, can effectively protect insect viruses from being killed by light, and improves the insecticidal efficiency.

The invention discloses a method for producing cobra snake poison protein (CT) and phospholipase A2 (PLA2) in a baculovirus-insect expression system. The method comprises the following steps: separating DNA (Deoxyribonucleic Acid) sequences expressing CT and PLA2 mature peptides from the poison gland of a cobra by using a RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method, and constructing insect virus expression vectors BM-CT and BM-PLA2; transferring a vector carrying a target gene and an empty vector into Sf9 insect cells to obtain virus particles; infecting the cells by using the virus particles, detecting the expression of the target protein through SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) electrophoresis, and purifying CT and PLA2 from cell lysis supernatant by using a Ni column elution method. The analgesic activities of CT and PLA2 are detected by using the abdominal constriction test of mice. As proved by a detection result, the twisting times of the mouse in a CT treatment group are remarkably less than that of a PLA2 treatment group and a control group, indicating that the obtained CT has analgesic activity and has a better effect than PLA2; the twisting times of the mouse in the PLA2 treatment group are remarkably less than that of the control group, indicating that the obtained PLA2 also has analgesic activity. The related CT and PLA2 proteins can be taken as analgesics for application to the development and application of clinical medical analgesics as well as the research of foundation medicine.

The invention relates to a preparation of an ultraviolet-resistant inactivating insect virus and belongs to the field of green agricultural biological prevention and control. The invention aims to solve the technical problems that the present insect virus is easy to inactivate at outside and poor in stability. The preparation is realized according to the following method: 1) preparing chitosan-virus mixed solution; 2) preparing an oil phase; and 3) biologically preparing a chitosan microcapsule. The ultraviolet-resistant inactivating insect virus according to the invention is capable of reducing the environmental pollution, promoting the use stability and reducing the production cost, and meanwhile, is capable of effectively prolonging the pesticide effect duration of the insect virus andis applicable to the fields of control on crop growth, crop pest killing, and the like.

The invention discloses a soil repairing agent for neutralizing soil heavy metal with insect viral protein. The soil repairing agent comprises the following ingredients, by weight, 0.5-5% of insect nuclear polyhedrosis virus granular mother liquor, 35-55% of grass carbon, 5-10% of potassium dihydrogen phosphate, 5-20% of calcium carbonate and 5-20% of sodium silicate. Insect viral protein has an efficient repairing effect on heavy metal in soil. In addition, source of each ingredient is natural. The soil repairing agent is highly safe to the environmental, and secondary pollution will not be caused. The soil repairing agent is convenient to manufacture, and costs are low.

The invention discloses an insect cell line which is derived from a cabbage looper embryonic cell line QAU-Tn-E-7. The collection number of the cell line is CCTCC NO: C201865. The cell line is suitable for serum-free suspension culture, free from TNCL viruses and high in growth speed, cell density, viral yield and protein expression level under the condition of serum-free suspension culture. The cell line is an ideal cell line for producing insect virus insecticides and exogenous recombinant proteins on a large scale.

The invention provides a process for producing a virus biological insect-proofing organic fertilizer, which relates to the technical field of biological treatment of livestock excrements and life sewage. The process employs the following steps: preparing a raw material, propagating insect virus, collecting the insect virus and the organic fertilizer raw material, mixing, drying, crushing, examining and packaging for warehousing. The process for producing a virus biological insect-proofing organic fertilizer can effectively prevent and treat soil insects, such as cutworm, wireworm, Chinese mole cricket, grub, vegetable and fruit root-knot nematode disease, rice-stem borer and yellow rice borer, so that an organic matter can be increased, the soil granule structure is improved, the soil fertility can be improved by culturing fertilizer, the crops quality is improved, the unit area output is increased, and the ecological balance can be recovered.

The invention belongs to the technical field of tea tree insect disease prevention and discloses a method of preventing Basilepta melanopus Lefevre. The method comprises steps of plowing the soil and trenching, trimming and backfilling tea trees, additionally applying biological agent fertilizers, covering a mulching film and irrigating. By the use of trimming measurements and the additional application of the biological agent fertilizers, tea output reduction due to trimming, great artificial reproduction release technological difficulty, low insect-virus pesticide efficiency and environmental pollution due to the use of chemical pesticides in the traditional agricultural prevention can be overcome. Damaged plant rate can be reduced to 2% and each branch only has two leaf holes when the method is applied to prevention of the Basilepta melanopus Lefevre, so damaged amount can be obviously reduced. Soil can be improved and insects can be prevented; nutrients required by tea trees can be increased; direct meaning is provided for output and income increase and environment protection; and important reference value is provided for re-use of tobacco stems, tea bran and tea tree trimmed branches and the realization of circular economy of reutilizing wastes.

The invention discloses a virus insecticidal suspension agent containing abamectin. The virus insecticidal suspension agent comprises the following components in percentage by weight: 0.1-10% of abamectin, 1-10% of a dispersant, 1-7% of an antifreezing agent, 0.2-10% of a stabilizer, 0.1-5% of a synergist, 0.5-2% of an ultraviolet protecting agent, 0.1-2% of a defoaming agent, 0.1-3% of a pH regulator and the balance of water, wherein the prepared suspension agent also contains an insect virus which is one of or a combination of a nuclear polyhedrosis virus (NPV) and granulosis virus, and the content of an insect virus inclusion body in the suspension agent is 0.5-20 billion PIB / ml. The abamectin and a virus insecticide of the virus insecticidal suspension agent disclosed by the invention are subjected to complex pairing, so that the problem of insect resistance can be solved, and the virus insecticidal suspension agent is safe and environmentally friendly.

The invention discloses a preparation method of gelatin / CMC insect virus microcapsules. The method comprises the following steps: (1) dissolving CMC in deionized water to obtain a CMC solution; addingan insect virus dry powder into the CMC solution, and performing stirring and mixing uniformly to form a mixture A; and preparing a gelatin solution with a mass percentage of 1%-5% by using a ready-made buffer solution; and (2) adding the gelatin solution into the mixture A, wherein a mass ratio of gelatin to CMC is 1:2-12:1; performing stirring at a temperature of 25-35 DEG C with a revolution speed of 200-1000 r / min until the solution is mixed uniformly; and dropwise adding a glacial acetic acid solution with a mass percentage of 10% into the solution, slowly adjusting a pH value of the solution until spherical microcapsules appear in the solution observed under a microscope, stopping heating, performing cooling, dropwise adding a curing agent solution, carrying out a reaction for 30-60min, performing standing separation, removing a supernatant, and freeze drying a precipitate to obtain the gelatin / CMC virus microcapsules. The gelatin / CMC virus microcapsules can be applied to the Spodoptera litura polyhedrosis virus, is safe and environment-friendly, has strong anti-ultraviolet performance, can effectively protect the virus from being killed by ultraviolet rays, and improves aninsecticidal rate.

A process for increasing the reproduction efficiency of insect virus is characterized by that the synergistic protein of insect virus and the inclusion body of insect virus are exploded to obtain virus particles, which are used to infect the adult insects for high-efficiency and high-level reproduction of said virus in the insects.

The invention provides a self-propagation device and method of nuclear polyhedrosis viruses of beet armyworm. The self-propagation device comprises a reflection lampshade, a powder storage mechanism and an inhabitation platform which is arranged on the bottom of the powder storage mechanism. The powder storage mechanism comprises an inner cylinder and an outer cylinder which is in threaded connection with the inner cylinder, a first cavity is formed in the inner cylinder, a first opening communicated with the first cavity is formed in the lower end of the inner cylinder, a second cavity is formed in the outer cylinder, and a second opening allowing the inner cylinder to stretch into the second cavity is formed in the top of the outer cylinder. A bottom plate is arranged on the bottom of the outer cylinder, and one or more powder outlets are formed in the outer side face at the lower end of the outer cylinder; a sex attractant is arranged in the inner cylinder. Insect virus powder is stored into the powder storage mechanism, and flows out of the powder outlet in the outer cylinder to the inhabitation platform. When lured by light or pheromones to carry out activities on the propagation device, insects can inhabit on the inhabitation platform, and after leaving off, the insects can carry insect viruses on the inhabitation platform and enter an environment, so that propagation ofthe insect viruses is achieved.

The invention discloses a method for synergizing the beet armyworm nuclear polyhedrosis virus. Aiming at the defects of slow insecticidal speed and low toxicity of a single natural insect virus, the joint action of insect larvae parasitoids and viruses is used to treat the beet armyworm at the same time with the beet armyworm nucleopolyhedrosis virus, or parasitize first Bee treatment and virus treatment of beet armyworm can significantly increase the infectivity of the virus to pests. Compared with the traditional use of neurotoxic chemical pesticides to increase the effect of virus control, this technology uses the joint action of the larvae parasitic wasp scutellaria and the virus, which not only has outstanding synergistic effect, but also has high safety, which can effectively improve the biological control of pests. Level. After a large number of applications in production, the economic, social and ecological benefits will be very significant.

An integrated system of biological control of insect pests comprises a fungal breeding releaser, an insect trap, an insect virus auxiliary self-propagating device and a solar power supply device. Thesolar power supply device can supply power to the light trap arranged on the insect trap, under the incentive of light in a specific wavelength, a part of the insects entering into the insect collecting device, falling into the fungal breeding release bag, infected by the fungus in the fungal breeding release bag to carry on continuous reproduction of fungus, and when there is more fungus, the fungal spore breaks through the release bag and is released into the environment, thus infecting other pests. A part of the insects which do not enter into the insect trap carry the insect viruses into the environment by stopping on the insect virus auxiliary self-propagating device so as to achieve the self-propagation purpose of the insect viruses. The light trap, virus biological control and fungal insecticide are applied together in the whole device to achieve the purposes of effective and continuous biological control.