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Method for splitting insect body by utilizing insect virus to extract insect peptide

An insect virus and insect technology, applied in the field of polypeptide extraction, can solve the problems of severe conditions, loss of activity, high energy consumption, etc., and achieve the effect of simple process, remarkable effect and mild conditions

Inactive Publication Date: 2015-03-04
JIANGXI NEW DRAGON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The conditions of this conventional method are severe, and many highly active polypeptides in insects cannot tolerate such harsh conditions, and will lose their activity under the high temperature conditions of strong acid and strong alkali
At the same time, this method pollutes the environment and consumes a lot of energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 300 cotton bollworms and evenly spray the nuclear polyhedrosis virus of cotton bollworm on the feeding feed; 7 days after the cotton bollworm eats the virus, the worm body becomes soft and begins to hydrate; at this time, the cotton bollworm is collected; after the worm body is completely hydrated , pass the hydration liquid mixture through a 150-mesh sieve to remove insect skin and other impurities; centrifuge the filtrate; separate the centrifuged lower layer of virus, and the upper layer of liquid is the water-soluble polypeptide solution from insects.

[0019] The water-soluble polypeptide solution of the source insect body was mixed with water 100 times, directly sprayed on the plants, and a clear water control group was set. Has a significant growth-promoting effect.

Embodiment 2

[0021] Get 300 Spodoptera litura larvae, and evenly spray the nuclear polyhedrosis virus of cotton bollworm on the feeding feed; 7 days after the Spodoptera litura larvae eat the virus, the worm body becomes soft and begins to hydrate; Polypeptide solution to collect Spodoptera litura larvae; after the worms are completely hydrated, pass the hydration liquid mixture through a 150-mesh sieve to remove insect skins and other impurities; the filtrate is centrifuged; the centrifuged lower layer virus is separated, the upper layer The liquid is a water-soluble polypeptide solution derived from insects.

[0022] Use 100 milliliters of the polypeptide solution, add 25 grams of potassium dihydrogen phosphate, can be mixed with water 100 times, and directly spray on plants.

[0023] Add 25 grams of potassium dihydrogen phosphate to 100 milliliters of the water-soluble polypeptide solution of the source insect body, then add water 100 times, spray the plants directly, and set the potass...

Embodiment 3

[0025] Get 300 silkworm larvae, and evenly spray the silkworm nuclear polyhedrosis virus on the feeding feed; 7 days after the silkworm eats the virus, the worm body becomes soft and begins to hydrate; at this time, the silkworm is collected; after the worm body is completely hydrated, the water The liquid mixture is passed through a 150-mesh sieve to remove insect skin and other impurities; the filtrate is centrifuged; the centrifuged lower layer of virus is separated, and the upper layer of liquid is the water-soluble polypeptide solution from insects.

[0026] Use 100 milliliters of the polypeptide solution, which can be mixed with water 100 times and sprayed directly on plants.

[0027] 100 milliliters of the water-soluble polypeptide solution of this source insect body, add 100 grams of urea, then add water 100 times, directly spray to plants, set urea solution control group, the experiment proves that plant growth is obviously better than urea solution control group, ther...

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Abstract

The invention discloses a method for splitting an insect body by utilizing an insect virus to extract insect peptide. A living insect body is fed with the corresponding insect virus; the insect virus is greatly duplicated in the insect body, and a great number of proteolytic enzymes are generated; the structural protein of the insect body can be hydrolyzed into water-soluble peptide by the proteolytic enzymes, so that the insect body is hydrated; after an insect dies from hydration, a liquid mixture A containing an insect skin which is not hydrolyzed by the proteolytic enzymes, the insect virus and the water-soluble peptide is obtained; the insect skin in the liquid mixture A is filtered by a screen, and a liquid mixture B is obtained; the liquid mixture B is subjected to centrifugal treatment; a water-soluble peptide solution positioned on an upper centrifugal layer is separated. The water-soluble peptide solution which is obtained through the method and comes from the insect body is rich in the water-soluble peptide, and has a remarkable effect when serving as a plant foliar fertilizer; in addition, the process of the method is simple, the conditions are mild, and the method is economical, environmentally-friendly and efficient.

Description

technical field [0001] The invention relates to the field of polypeptide extraction, in particular to a method for extracting insect polypeptides by using insect viruses to lyse insect bodies. Background technique [0002] Insects are rich in various amino acids, polypeptides and proteins. Experiments have found that the polypeptide of the insect body has beneficial nutrition and growth-promoting effects on the growth of plants. How to efficiently and gently extract active peptides from insects is a meaningful topic. [0003] The usual method for extracting insect body polypeptides is to use strong acid and strong base, such as concentrated sulfuric acid and concentrated sodium hydroxide under high temperature conditions, to hydrolyze the water-soluble polypeptides in the insect body, and then collect these hydrolyzed polypeptide products. [0004] The conditions of this conventional method are severe, and many highly active polypeptides in insects cannot tolerate such...

Claims

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Application Information

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IPC IPC(8): C12P21/06C05F1/00C05G1/00
CPCY02A40/20
Inventor 邓方坤
Owner JIANGXI NEW DRAGON BIOTECH
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