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Use of cnidiadin as insect virus synergist in biological control

A technology of osthole and insect virus, applied in the application, biocide, animal repellent and other directions, can solve the problems of difficult to popularize and apply virus enhancer, difficult to popularize and apply, low environmental safety, etc., and achieve a simple and mature extraction process. , Significant economy, low dosage effect

Inactive Publication Date: 2005-08-03
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above findings have increased the possibility of practical use of viruses, both types of virus boosters have their own advantages and disadvantages
Among them, virus enhancer is derived from insect virus, and as a biosynergist, it has high safety. However, in the production process, virus enhancer and the enhanced virus cannot proliferate in the same host insect, and need to be produced in two different After the specific host insects are proliferated separately, the preparations are mixed and used, which greatly increases the production cost and makes it difficult to popularize and apply the virus enhancer as a synergist
Although recombinant viruses and transgenic plants that can express enhancers have been constructed through genetic engineering, it is difficult to popularize and apply them in the short term due to many factors such as expression stability and safety of transgenic organisms.
As a chemical synergist, stilbene fluorescent whitening agent has a wide synergistic spectrum and high synergistic activity, but due to its high concentration (10000mg / L), the application cost is relatively high, especially the environmental safety Low and thus not an ideal viral booster

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the synergistic effect of osthole on the insecticidal virulence of AcNPV

[0020] Respectively measure the insecticidal activity of osthole and AcNPV single agent to pests and show that no matter use 20% osthole crude extract or prepare liquid medicine with 99% osthole pure product, single use osthole 1-228mg / L It has no insecticidal effect on beet armyworm and Spodoptera litura, but AcNPV3.3×10 alone 4 -133×10 4 The insecticidal effect of PIB / ml on the 2nd instar beet armyworm was 8.1%-84.2%, 6.6×10 4 -266×10 4 The insecticidal effect of PIB / ml on the third instar beet armyworm was 20.5%-95.2% (Table 1, 2).

[0021] Table 1 Insecticidal activity of different concentrations of osthole against beet armyworm and Spodoptera litura

[0022] Age Concentration (mg / L) Insecticidal effect of crude extract (%) Insecticidal effect of pure product (%)

[0023] Beet armyworm Spodoptera litura Beet armyworm Spodoptera litura

[0024] 2 y...

Embodiment 2

[0067] Embodiment 2, osthole synergistic effect on SlNPV desinsection

[0068] Crude osthole extract also improved the insecticidal toxicity of SlNPV to Spodoptera litura (Table 5). Single use of 7-114mg / L osthole was ineffective against Spodoptera litura, but 7mg / L osthole could increase the toxicity of SlNPV to 2nd and 3rd instar larvae of Spodoptera litura, and the synergistic multiples were 4.67 and 2.55 times, respectively.

[0069] Table 5 Osthole acts on the synergistic effect of SlNPV on Spodoptera litura

[0070] Age Treatment Slope LC 50 (95%CL)(×10 6 PIB / ml) synergistic ratio

[0071] 2nd SlNPV 1.5469 17.39(13.43-22.25) 1.00

[0072] SlNPV+7mg / L 1.2036 3.72(2.32-5.34)** 4.67

[0073] 3rd SlNPV 1.3578 28.84(21.26-32.20) 1.00

[0074] SlNPV+7mg / L 1.1023 11.30(2.45-3.79)* 2.55

Embodiment 3

[0075] Embodiment 3, the synergistic effect of osthole on the insecticidal virulence of MbNPV

[0076] The crude extract of osthole can also increase the insecticidal toxicity of MbNPV to Brassica cabbage worm (Table 6). Using 7-228mg / L osthole alone has no insecticidal effect on cabbage armyworm, but when 7mg / L osthole is mixed with MbNPV, the insecticidal toxicity of MbNPV on cabbage armyworm is significantly improved, and the synergistic multiple is up to 5.71 times.

[0077] Table 6 The synergistic effect of osthole on MbNPV acting on cabbage armyworm

[0078] Process Slope LC 50 (95%CL)(×10 6 PIB / ml) synergistic ratio

[0079] MbNPV 1.4852 28.68(16.52-37.12) 1.00

[0080] MbNPV+7mg / L 1.1589 5.02(3.92-5.84)** 5.71

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PUM

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Abstract

The invention is an application of cnidiadin as insect virus synergist to the biologic prevention and cure. Cnidiadin derives from common cnidium fruit extract, when used singly, has not pesticidal activity, but when associated with insect nuclear polyhedrosis virus (AcNPV, SINPV, MbNPV, etc.) and granulosis virus (XcGV, etc.), can remarkably enhance the infection capability of insect virus to pest. The dosage is low (1-228mg / L), and the concentration lower, the synergistic action stronger, safe to entironment.

Description

1. Technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to the application of a cnidium plant extract as a synergist for insect viruses in biological control. 2. Background technology [0002] As a kind of biological insecticide, insect virus mainly includes nuclear polyhedrosis virus (NPV) and granular virus (GV). Because of its high pathogenicity and specificity to host insects, it is safe to humans, animals and natural enemies. It does not pollute the environment, especially it can form an epidemic in the pest population and control the insect population for a long time, and it is not easy to produce drug resistance, etc., which has always attracted people's attention. According to statistics, more than 300 species of NPV have been isolated and more than 70 species of GV have been isolated, of which more than 20 species have been introduced into field control trials, and more than 10 species have been made in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N31/08A01N43/08
Inventor 方继朝郭慧芳王节萍刘宝生
Owner JIANGSU ACAD OF AGRI SCI
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