Preparation method of gelatin/CMC insect virus microcapsules
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A technology of insect virus and microcapsules, which is applied in botany equipment and methods, insecticides, animal repellants, etc., can solve the problems affecting the application of insect viruses and insufficient anti-ultraviolet performance, so as to improve the insecticidal rate and anti-ultraviolet Strong UV effect
Pending Publication Date: 2020-01-24
ZHONGKAI UNIV OF AGRI & ENG
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Insufficient UV resistance of existing insecticides f...
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Embodiment 1
[0026] A preparation method for gelatin / CMC insect virus microcapsules, comprising the following steps:
[0027] (1) Dissolve CMC in deionized water to obtain a CMC solution with a mass fraction of 1%; add 0.1 g of virus dry powder to 50 g of CMC solution, stir and mix evenly to form mixture A; prepare a mass fraction of 1 with the ready-to-use buffer solution % gelatin solution;
[0028] (2) Add gelatin solution to mixture A, wherein the mass ratio of gelatin to CMC is 9:1; stir until the solution is mixed evenly at a temperature of 35°C, and the rotating speed is 400r / min; the mass percentage is 10% added dropwise to the solution glacial acetic acid solution, slowly adjust the pH value of the solution until the pH value is 4.632 (at this time, draw 1ml of the solution and save it as sample 1), stop heating, cool the solution, add 2ml of 1mg / ml tea polyphenol aqueous solution dropwise, react for 30min, Leave to stand and centrifuge, remove the supernatant, and freeze-dry the...
Embodiment 2
[0031] A preparation method for gelatin / CMC insect virus microcapsules, comprising the following steps:
[0032] (1) Dissolve CMC in deionized water to obtain a CMC solution with a mass fraction of 1%; add 0.5 g of virus dry powder to 100 g of CMC solution, stir and mix evenly to form mixture A; prepare a mass fraction of 1 with the ready-to-use buffer solution % gelatin solution;
[0033] (2) Add gelatin solution to mixture A, wherein the mass ratio of gelatin to CMC is 2:1; stir at 30°C until the solution is evenly mixed, and the rotating speed is 600r / min; dropwise into the solution, the mass percentage is 10% glacial acetic acid solution, slowly adjust the pH value of the solution until the pH value is 4.632, stop heating, let the solution cool down, add 1ml of 0.5mg / ml formaldehyde solution dropwise, react for 30min, let stand and centrifuge, remove the supernatant, and freeze the precipitate Dried is gelatin / CMC virus microcapsule.
[0034] The virus dry powder is Spod...
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Abstract
The invention discloses a preparation method of gelatin/CMC insect virus microcapsules. The method comprises the following steps: (1) dissolving CMC in deionized water to obtain a CMC solution; addingan insect virus dry powder into the CMC solution, and performing stirring and mixing uniformly to form a mixture A; and preparing a gelatin solution with a mass percentage of 1%-5% by using a ready-made buffer solution; and (2) adding the gelatin solution into the mixture A, wherein a mass ratio of gelatin to CMC is 1:2-12:1; performing stirring at a temperature of 25-35 DEG C with a revolution speed of 200-1000 r/min until the solution is mixed uniformly; and dropwise adding a glacial acetic acid solution with a mass percentage of 10% into the solution, slowly adjusting a pH value of the solution until spherical microcapsules appear in the solution observed under a microscope, stopping heating, performing cooling, dropwise adding a curing agent solution, carrying out a reaction for 30-60min, performing standing separation, removing a supernatant, and freeze drying a precipitate to obtain the gelatin/CMC virus microcapsules. The gelatin/CMC virus microcapsules can be applied to the Spodoptera litura polyhedrosis virus, is safe and environment-friendly, has strong anti-ultraviolet performance, can effectively protect the virus from being killed by ultraviolet rays, and improves aninsecticidal rate.
Description
technical field [0001] The invention relates to the technical field of slow-release microcapsule pesticides, in particular to a preparation method of gelatin / CMC insect virus microcapsules. Background technique [0002] The country is currently committed to developing a healthy China, advocating green development, and attaching great importance to ecological and environmental protection. Therefore, traditional pesticides with high toxicity, high pollution, difficult storage, poor physical and chemical stability, and low utilization rate can no longer meet the needs of people today, which makes people pay more attention to pesticide mitigation while researching and inventing new pesticide products. Research on release and controlled release. [0003] Microcapsule technology uses natural or synthetic polymer materials as the capsule wall, and wraps the active substance (capsule core) by chemical, physical or physicochemical methods to form a semi-permeable or sealed capsule m...
Claims
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