The invention provides a preparation and application of an adipose-derived stem cell exosome in facial rejuvenation. The preparation method comprises the following steps: separation of adipose-derivedstem cell, extraction of the exosome and coating of adipose-derived stem cell exosome-nano collagen. By carrying out facial microscopic carving and face-lifting by virtue of the adipose-derived stemcell exosome-nano collagen, the superiorities of the adipose-derived stem cell exosome-nano collagen, compared with adipose-derived stem cell autoplastic transplantation, exosome injection or pure nano collagen, in skin reconstructive repairing and micro-face lifting are observed. Particularly, adipose-derived stem cells are separated, the exosomes of the adipose-derived stem cells are extracted,the nano collagen is fused with the separated exosome to form an adipose-derived stem cell exosome-nano collagen combined preparation, the combined preparation is injected into the skin an injured part of a New Zealand white rabbit, the promotion effect of the adipose-derived stem cell exosome-nano collagen, compared with a pure adipose-derived stem cell transplantation group, an exosome group andpure nano collagen, in the regeneration of injured skin is observed, and meanwhile, a user can observe whether the adipose-derived stem cell exosome is harmful to a human body.