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30 results about "Egg drop syndrome virus" patented technology

GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases

The invention discloses a GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases. The invention provides a GeXP detection primer group for identifying or assisting to identify duck infectious disease pathogens, wherein the primer group consists of a primer pair A, a primer pair B, a primer pair C, a primer pair D, a primer pair E, a primer pair F, a primer pair G, a primer pair H, a primer pair I, a primer pair J, a primer pair K and a primer pair L. According to the GeXP detection kit, shown by experiments, the primer group, a PCR (Polymerase Chain Reaction) reagent and the primer pairs, provided by the invention, are used for simultaneously differentiating and detecting avian influenza viruses, H5, H7 and H9 subtype avian influenza viruses, duck hepatitis viruses, duck plague viruses, duck flaviviruses, newcastle disease viruses, egg drop syndrome viruses, muscovy duck reoviruses, muscovy duck parvoviruses and duck circoviruses and are good in specificity and high in sensitivity. The detection kit, which is simple and convenient and is high in flux, and a detection system are provided for the detection on common major duck infectious disease pathogens, so that the practical needs are better met, and application prospects are broad.
Owner:GUANGXI VETERINARY RES INST

GeXP Detection Kits for Identification of 11 Kinds of Duck Virus Diseases

Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The can kit detect, simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.
Owner:GUANGXI VETERINARY RES INST

Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine

The invention relates to a method for producing a newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine. A newcastle disease virus La Sota strain, a gallinaceous infectious bronchitis virus M41 strain, an egg drop syndrome virus NE4 strain and an A-type avian influenza virus (H9 subtype) YBF003 strain serve as production virus strains, and are mixed with a high-quality adjuvant to be prepared into the inactivated vaccine through a super concentration process; after an animal is immunized by the vaccine, an antibody is rapidly produced, the titer is high, the protection period is long, and occurrence and spread of diseases are reduced.
Owner:YEBIO BIOENG OF QINGDAO

Chinese medicinal composition treating duck Tembusu virus

Belonging to the field of traditional Chinese medicines, the invention particularly relates to a Chinese medicinal composition treating a duck Tembusu virus. The Chinese medicinal composition is prepared from the following raw materials by weight: 45g of Schizonepeta, 30g of radix sileris, 25g of notopterygium roots, 25g of radix angelicae biseratae, 30g of radix bupleuri, 25g of Radix Peucedani, 30g of fructus aurantii, 45g of Poria cocos, 30g of Platycodon grandiflorum, 25g of Ligusticum wallichii, 15g of liquorice, 15g of mint, 30-50g of Venenum Bufonis, 40-80g of radix isatidis, 50-100g of folium artemisiae argyi, 30-50g of Polygonum multiflorum, 40-80g of hawthorn, and 40-80g of dried tangerine peel. The Chinese medicinal composition provided in the invention has very good efficacy on poultry egg laying decrease, inappetence, depression and diarrhea caused by the duck Tembusu virus, an influenza virus and an egg drop syndrome virus, and especially has significant efficacy on sudden egg drop syndrome caused by duck Tembusu virus infection.
Owner:余姚市禽畜病防治研究所

Quadruple inactivated vaccine for preventing chicken diseases

The invention discloses a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively vaccinating Newcastle disease virus ZM10 strain, infectious bronchitis virus M41 strain and H9 subtype avian influenza virus S2 strain in a susceptible chicken embryo, and collecting an infected chicken embryo solution; vaccinating egg drop syndrome virus AV-127 strain in a susceptible duck embryo, and collecting an infected duck embryo solution; and concentrating the collected virus solutions, inactivating, blending with an oil adjuvant, and emulsifying. The quadruple vaccine provided by the invention can be used for preventing diseases caused by Newcastle disease viruses, infectious bronchitis viruses, egg drop syndrome viruses and avian influenza (H9 subtype) viruses to achieve the effect of preventing four diseases simultaneously and reduce immune cost and immune stress reaction.
Owner:乾元浩生物股份有限公司

Triple fluorogenic quantitative PCR detection primer, kit and method of DTMUV, EDSV and H9 subtype AIV

The invention discloses a triple fluorogenic quantitative PCR detection primer, kit and method of the duck tembusu virus disease, the egg drop syndrome virus and the H9 subtype avian influenza virus. The detection primer for the duck tembusu virus disease, the egg drop syndrome virus and the H9 subtype avian influenza virus is shown in SEQ ID NO.1-6. The detection primer and kit can detect the EDSV, the H9 AIV and the DTMUV at the same time. No cross reaction with duck plague virus, goose parovovirus, muscovy duck parvovirus and escherichia coli genomic DNA and duck hepatitis A virus 1, duck hepatitis A virus 3, muscovy duck reovirus and newcastle disease virus RNA exists. The lower limits of detection for EDSV nucleic acid, H9 AIV nucleic acid and DTMUV nucleic acid are 8.0 copies, 4.8 copies and 1.3 copies respectively. The detection primer, kit and method can be used for qualitative and quantitative detection for the EDSV, the H9 AIV and the DTMUV.
Owner:INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI

Vaccine composition for preventing egg drop syndrome, and preparation method and application of vaccine composition

The invention relates to a vaccine composition. The vaccine composition comprises an immunological dose of egg drop syndrome virus Penton protein or an immunological dose of a live vector containing arecombinant egg drop syndrome virus Penton protein gene, and a veterinary acceptable carrier. The vaccine composition can effectively protect the occurrence of egg laying of chickens, and can completely prevent against wild strains of egg drop syndrome virus in different regions.
Owner:PU LIKE BIO ENG

Technique for concentrating chicken ND-IB-AI-EDS tetrad oil emulsion killed vaccine antigen

The invention provides a concentration technology of virus antigen during the preparation process of a chicken ND-IB-AI-EDS quadruple inactivated oil-emulsion vaccine. The main contents of the method are that: newcastle disease virus (NDV), infectious bronchitis virus (IBV) and H9 subtype avian influenza virus (AIV) are concentrated by four steps of rough filtration, fine filtration, ultrafiltration and material collection via an internal pressure hollow fiber ultrafilter. After the concentration, the three virus antigens are mixed with egg drop syndrome virus (EDSV) for the preparation of the chicken ND-IB-AI-EDS quadruple inactivated oil-emulsion vaccine.
Owner:TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY

Indirect ELISA (Enzyme-linked Immuno Sorbent Assay) kit for detecting blood serum 8 type avian adenovirus antibody based on spike glycoprotein F

The invention discloses an indirect ELISA (Enzyme-linked Immuno Sorbent Assay) kit for detecting a blood serum 8 type avian adenovirus antibody based on spike glycoprotein F. The indirect ELISA kit comprises an ELISA enzyme-linked plate which is coated with expression protein of blood serum 8 type avian adenovirus spike glycoprotein gene F, positive and negative controls, an HRP (Horseradish Peroxidase) labeled rabbit anti-chicken secondary antibody, a diluting solution, a color developing solution, a washing solution and a stopping solution. The ELISA kit disclosed by the invention can be used for specifically detecting a FAdV-8 antibody and does not react with anti-influenza virus blood serum, anti-Marek's virus blood serum, anti-Newcastle disease virus blood serum, anti-chicken infectious anemia virus blood serum, reticuloendotheliosis virus, ant-blood serum 4 type avian adenovirus blood serum, anti-egg drop syndrome virus blood serum and SFP chicken blood serum. Therefore, the kitdisclosed by the invention has good FAdV-8 specificity and can be used for FAdV-8 infection and immunization status epidemiological surveys.
Owner:YANGZHOU UNIV

Duplex fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection kit for duck tembusu virus and egg drop syndrome virus

ActiveCN104278106ARealize the purpose of one tube and two inspectionsSensitivity not affectedMicrobiological testing/measurementDNA/RNA fragmentationReverse transcriptaseFluorescence
The invention discloses a duplex fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection kit for duck tembusu virus and egg drop syndrome virus. The detection kit comprises a primer group and a probe group, wherein the primer group comprises primers 1-4, which have base sequences as shown in SEQ. ID. No. 1 and 2, as well as SEQ. ID. No. 4 and 5 in a sequence table respectively; the probe group comprises a probe A and a probe B, which have the base sequences as shown in SEQ. ID. No. 3 and 6 in the sequence table respectively. Experiments prove that the detection kit disclosed by the invention has the advantages of short detection time, high detection sensitivity and strong specificity, and can realize simultaneous detection and identification of two pathogens of duck tembusu virus and egg drop syndrome virus and quantify the corresponding pathogen content, so that the detection kit can be used for evaluating curative effects of duck tembusu virus and egg drop syndrome virus vaccines and medicaments and researching pathogenic mechanisms and other aspects, and further has important significance in prevention and treatment of duck tembusu virus and egg drop syndrome virus.
Owner:GUANGXI VETERINARY RES INST

Preparation method and applications of chicken Newcastle disease and egg drop syndrome bivalent genetic engineering subunit vaccine

InactiveCN108567976AProduction technology safetyProduction technology is stableSsRNA viruses negative-senseViral antigen ingredientsAdjuvantGenetic engineering
The invention provides a chicken Newcastle disease and egg drop syndrome bivalent genetic engineering subunit vaccine and uses thereof, and a preparation method thereof, wherein the preparation methodcomprises: fusing the HN fragment of chicken Newcastle disease virus and the knob fragment of egg drop syndrome virus through a linking peptide to form a vector, and expressing in mammalian cells. According to the present invention, with the matched application of the obtained genetic engineering subunit vaccine and the adjuvant to immunize chicken, the chicken Newcastle disease and egg drop syndrome bivalent genetic engineering subunit vaccine has good immunogenicity, can be safely applied to poultry, and can achieve good prevention effects.
Owner:成都金洛克锶生物技术有限公司

Combined inactivate vaccine for egg drop syndrome and avian encephalomlris and preparation method of combined inactivate vaccine

The invention provides a combined inactivate vaccine for the egg drop syndrome and avian encephalomlris and a preparation method of the combined inactivate vaccine, and relates to the field of bioengineering. The combined inactivate vaccine contains an inactivate egg drop syndrome virus AV127 and an avian encephalomlris virus HM08 strain, the conservation number of the HM08 strain is CGMCC No.7759. The preparation method of the vaccine is as follows: two virus strains are respectively inoculated on a susceptible duck embryo and a chick embryo SPF to obtain an egg drop syndrome virus solution and an avian encephalomlris virus solution; the inactivate virus solutions are mixed and then evenly mixed with a Twain-80 to prepare an aqueous phase, the aqueous phase and an oil phase are evenly mixed to obtain the combined inactivate vaccine. The HM08 strain propagates in the chick embryo SPF, the content of 0.2ml of the virus solution is more than or equal to 107.0EID50, the immunogenicity is good, the produced antibody level is high, the cross protection is good, and the combined inactivate vaccine produces a high-level antibody for a long persistent period.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis

The invention relates to a method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis. The inactivated vaccines are prepared by using new castle disease viruses, egg drop syndrome viruses and avian viral arthritis viruses as production strains and adopting an ultra strong concentration process and high-quality adjuvant; and the inactivated vaccines are quick in production of antibodies of immunized animals, high in effect and long in protection period, and reduce occurrence and propagation of epidemic diseases.
Owner:YEBIO BIOENG OF QINGDAO

Egg drop important virus loop-mediated multiplex PCR (polymerase chain reaction) quick detection primers

The invention provides egg drop important virus loop-mediated multiplex PCR (polymerase chain reaction) quick detection primers. The method comprises the following steps: preparing a specific trapping probe for nucleic acids of three avian egg drop important viruses (egg drop syndrome virus, avian Tembusu virus and avian influenza virus H9 subtype), hybridizing with the corresponding viral nucleic acid, cyclizing under the action of ligase, carrying out PCR (polymerase chain reaction) amplification detection by using a pair of probe-specific universal detection primers, and determining the infection pathogen according to the size of the obtained amplification product. The primers can be used for detecting a single virus, and can also be used for simultaneous differential diagnosis of three different viruses. Thus, the primers have the characteristics of high efficiency, high specificity, high sensitivity and low cost, are suitable for pathogen detection and analysis on abundant clinical samples in case of egg drop of the laying poultry, and provide an important technical means for early-stage quick differential diagnosis and molecular epidemiological analysis for determining egg-drop-causing pathogens.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI

Bigeminal multi-epitope vaccine for chicken infectious laryngotracheitis and egg drop syndrome

The invention relates to a bigeminal multi-epitope vaccine for chicken infectious laryngotracheitis and egg drop syndrome. Specifically, a gene recombination technology is used, 8 antigen epitope polypeptides related to chicken infectious laryngotracheitis virus gB, gD, and gE proteins as well as 6 antigen epitope polypeptides related to egg drop syndrome virus Penton and Fiber protein are connected with a molecular adjuvant IL-8, then are connected to a vector, transformed into host bacteria, and prepared by fermentation, purification and emulsification processes to obtain the bigeminal multi-epitope vaccine. The animal experiments show that the vaccine is safe and easy to use, and can induce significant humoral immunity and cellular immune responses in the body, the persistent period ofan antibody is long, and the infection of the infectious laryngotracheitis virus and egg drop syndrome virus can be effectively prevented.
Owner:QINGDAO MINGQIN BIOLOGICAL TECH CO LTD

Hen egg drop syndrome virus double-antibody sandwich ELISA detection method

The invention discloses a hen egg drop syndrome virus double-antibody sandwich ELISA detection method, specifically comprising the following steps: (1) immunizing a laying hen and a male healthy adultrabbit by using EDS76 as an antigen for preparing chicken anti-EDS76 serum and rabbit anti-EDS76 serum; (2) purifying anti-EDS76 immune globulin in the chicken anti-EDS76 serum and the rabbit anti-EDS76 serum to prepare chicken anti-EDS76 immune globulin and rabbit anti-EDS76 immune globulin; (3) performing detection by ELISA; (4) determining a critical value: take X+3S being equal to 0.331 as the determined critical value. The method provided by the invention overcomes the disadvantages of the traditional method that operation is cumbersome, time-consuming and labor-consuming, the detectionrate is low and the like, and solves the problem of persistent leaked detection of infected animals caused by the fact that only the antibody is checked but the virus is not checked. The results of specificity, crossover trials and repetitive tests show that the method is a specific, sensitive, rapid and easy-to-operate method.
Owner:ZHENGZHOU HOUYI PHARMA

An indirect ELISA kit for the detection of serotype 8 avian adenovirus antibodies based on spike protein f

The invention discloses an indirect ELISA (Enzyme-linked Immuno Sorbent Assay) kit for detecting a blood serum 8 type avian adenovirus antibody based on spike glycoprotein F. The indirect ELISA kit comprises an ELISA enzyme-linked plate which is coated with expression protein of blood serum 8 type avian adenovirus spike glycoprotein gene F, positive and negative controls, an HRP (Horseradish Peroxidase) labeled rabbit anti-chicken secondary antibody, a diluting solution, a color developing solution, a washing solution and a stopping solution. The ELISA kit disclosed by the invention can be used for specifically detecting a FAdV-8 antibody and does not react with anti-influenza virus blood serum, anti-Marek's virus blood serum, anti-Newcastle disease virus blood serum, anti-chicken infectious anemia virus blood serum, reticuloendotheliosis virus, ant-blood serum 4 type avian adenovirus blood serum, anti-egg drop syndrome virus blood serum and SFP chicken blood serum. Therefore, the kitdisclosed by the invention has good FAdV-8 specificity and can be used for FAdV-8 infection and immunization status epidemiological surveys.
Owner:YANGZHOU UNIV

Novel genetic engineering vaccine for poultry egg drop syndrome virus, its preparation method and application

The invention discloses a preparation method of an antigenic protein, comprising: respectively cloning the coding genes of codon-optimized recombinant avian egg drop syndrome virus Core protein, Fiber protein, Penton protein and Hexon protein into multiple recombinant shuttle vectors; Respectively transform the multiple recombinant shuttle vectors into competent host cells to obtain multiple recombinant baculovirus vectors; respectively transfect insect cells with the multiple recombinant baculovirus vectors to obtain recombinant baculoviruses; Co-infect insect cells to automatically assemble into VLPs in the cells and express antigenic proteins. The invention also discloses an immune composition containing the antigenic protein, which can be used to prepare a genetically engineered subunit vaccine of the avian egg drop syndrome virus. The structure, immunogenicity and function of the vaccine are similar to those of the natural protein, and the expression level is High, strong immunogenicity, no pathogenicity to animals, and can be prepared by large-scale serum-free suspension culture in a bioreactor, and the production cost is low.
Owner:苏州沃美生物有限公司

Vaccine composition containing Avian egg drop syndrome virus gene engineering subunit vaccine, preparation method for vaccine composition and application of vaccine composition

The invention provides a nucleotide sequence represented by SEQ ID NO. 2 or an Avian egg drop syndrome virus tFiber protein fragment encoded by a degenerate sequence of the nucleotide sequence and a vaccine composition prepared from the protein fragment. The fragment has good immunogenicity, can generate a good immunization effect in case of a low immunization amount and has the protective rate of 100%, the immunogenicity is better than that of a Fiber full-length protein, and a combined vaccine can be prepared from the fragment and a variety of antigens so as to generate complete protection on chickens and ducks.
Owner:PU LIKE BIO ENG

Novel genetic engineering vaccine for avian egg drop syndrome virus as well as preparation method and application of novel genetic engineering vaccine

The invention discloses a preparation method of antigen protein. The preparation method comprises the following steps of cloning encoding genes of codon-optimized recombinant avian egg drop syndrome virus Core protein, Fiber protein, Penton protein and Hexon protein onto a plurality of recombinant shuttle vectors; transforming competent host cells by the plurality of recombinant shuttle vectors to obtain a plurality of recombinant baculovirus vectors; transfecting insect cells with the plurality of recombinant baculovirus vectors to obtain recombinant baculoviruses; and co-infecting insect cells with the recombinant baculoviruses, so that the recombinant baculoviruses are automatically assembled into VLP in the cells, and the antigen protein is expressed. The invention also discloses an immune composition containing the antigen protein. The immune composition can be used for preparing an avian egg drop syndrome virus genetic engineering subunit vaccine; and the vaccine is similar to natural protein in structure, immunogenicity and function, high in expression level and strong in immunogenicity, has no pathogenicity to animals, can be subjected to large-scale serum-free suspension culture preparation through a bioreactor, and is low in production cost.
Owner:苏州世诺生物技术有限公司

Egg drop syndrome vaccine and preparation method thereof

The invention relates to the technical field of poultry vaccine and specifically relates to an egg drop syndrome vaccine and a preparation method thereof. The egg drop syndrome vaccine includes the inactivated antigen solution of eggs drop syndrome virus and an vaccine adjuvant. According to the invention, the antigen solution used for the vaccine is of high HA titre and low cost. The preparationmethod of the egg drop syndrome vaccine includes the following steps: thawing and culturing full suspended duck-embryo retinal cells, inoculating the cultured full suspended duck-embryo retinal cellswith the eggs drop syndrome virus (K-11) and adding a DMEM culture medium at the same time to perform culturing, and then obtaining virus and performing freeze-thawing. According to the invention, thevaccine prepared by the method is of higher titre and lower cost.
Owner:成都史纪生物制药有限公司

GeXP detection kits for identification of 11 kinds of duck virus diseases

Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The kit can detect simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.
Owner:GUANGXI VETERINARY RES INST

Method for detecting egg drop syndrome viruses and kit for method

The invention provides a method for detecting egg drop syndrome viruses and a kit for the method. By means of sequencing and comparing egg drop syndrome virus genes, a genetic marker for detecting the egg drop syndrome viruses, a real-time fluorescent quantitative PCR (polymerase chain reaction) primer and a probe are provided, and nucleotide sequences are respectively showed as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention further provides a real-time fluorescent quantitative PCR method for detecting the egg drop syndrome viruses and the detecting kit. The minimum detectable amount of the detection method reaches 10 copy / muL, the method has a good linear relation within a 1.0X102-1.0X108 copy / muL detection range, and R2 equals to 0.992. The method has the advantages of accurate detection, high sensitivity and specificity, simplicity, convenience and speediness, and has good specimen detection ability.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Duck Tembusu virus and Egg Drop Syndrome Virus Duplex Fluorescent Quantitative RT-PCR Detection Kit

ActiveCN104278106BShort detection timeRealize the purpose of one tube and two inspectionsMicrobiological testing/measurementDNA/RNA fragmentationTembusu virusFluorescence
The invention discloses a duplex fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection kit for duck tembusu virus and egg drop syndrome virus. The detection kit comprises a primer group and a probe group, wherein the primer group comprises primers 1-4, which have base sequences as shown in SEQ. ID. No. 1 and 2, as well as SEQ. ID. No. 4 and 5 in a sequence table respectively; the probe group comprises a probe A and a probe B, which have the base sequences as shown in SEQ. ID. No. 3 and 6 in the sequence table respectively. Experiments prove that the detection kit disclosed by the invention has the advantages of short detection time, high detection sensitivity and strong specificity, and can realize simultaneous detection and identification of two pathogens of duck tembusu virus and egg drop syndrome virus and quantify the corresponding pathogen content, so that the detection kit can be used for evaluating curative effects of duck tembusu virus and egg drop syndrome virus vaccines and medicaments and researching pathogenic mechanisms and other aspects, and further has important significance in prevention and treatment of duck tembusu virus and egg drop syndrome virus.
Owner:GUANGXI VETERINARY RES INST
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