59 results about "Dermodesis" patented technology

Dermatophytes which belong to one of the three genera Epidermophyton, Trichophyton and Microsporum are the main cause of fungal infections of skin, hair and nails. Traditional diagnostic procedures consist of microscopy and culture, but due to the slow growth rate of dermatophytes typically two to four weeks are needed before a final diagnosis is obtained. The present invention is a rapid DNA extraction method extracting nucleic acids from fungi (e.g. dermatophytes and other pathogenic fungi) which can be performed from directly on hair, nail or skin specimens from humans, from naturally or experimentally infected animals or from cultured fungal colonies for the use in PCR amplification and detection assays. The present invention also includes specific primer sets for detection of any dermatophyte and for species specific detection of Trichophyton rubrum and Epidermophyton floccosum by PCR and a kit for diagnosing fungal infections.

Mixed vesicular system containing a nanoemulsion with bilayer nanovesicles mimicking noisome and liposome for the topical delivery of dithranol alone or together with salicylic acid to treat psoriasis, eczemas, dermatophytoses, alopecia areata and other dermatological diseases and methods for producing said system.

An antifungal composition and penetrating carrier system for topical treatment of dermatophytic infection and secondary bacterial infections. The antifungal composition includes various fungistatic and fungicidal essential oil components, or combinations thereof. The penetrating carrier system may include various ingredients, including a penetration enhancer, such as isopropyl myristate.

The invention provides a method for identifying five dermatophytes by utilizing a high-resolution melting curve and belongs to the technical field of high-resolution melting curve analysis. The methodadopts a pair of specific primers and is based on real-time fluorescence PCR melting curve to identify trichophyton rubrum, trichophyton digitorum, epidermophyton floccosum, microsporum caninum and Microsporum incurvatum. The sequence of the pair of specific primers is as shown as SEQ ID NO.1-2. The method has the advantages of high sensitivity, good specificity and high detection speed, can be used for identifying dermatophytes on the aspects of clinical diagnosis, environmental monitoring, food safety and the like, and provides a reliable basis for infection treatment, environmental sanitation and food safety monitoring of dermatophytes.

A liquid carrier for topical administration is provided that contains a mineral oil, turpentine and, optionally, camphor, and optionally other terpenoid components containing pinene, and its use to provide an antifingal composition a mixture of antifungal essential oils and the carrier system, wherein the mixture of antifungal essential oils includes an effective amount of each of eucalyptus globulus, peppermint, cedarwood, and Manuka; along with the use of this composition in the topical treatment of fungal infection, particularly of the nail.

The invention discloses a polyrotaxane-structured NO donor material and a preparation method and application thereof. The polyrotaxane-structured NO donor material is an internal lock supramolecule formed by sleeving a dumbbell-shaped PEG-PPG-PEG linear molecule with a cationized beta-cyclodextrin molecule, wherein the beta-cyclodextrin molecule can freely slide on the PEG-PPG-PEG linear molecule.The internal lock supramolecule serving as an NO donor material can effectively inhibit growth and reproduction of bacteria and fungi, has a remarkable inhibiting effect on common pathogenic bacteria, dermatophytes, wound infection bacteria and the like, has functions of promoting wound healing, diminishing inflammation and the like, and can be applied to preparation of biomedical engineering materials.

The invention relates to a traditional Chinese medicinal composition capable of dispelling wind and eliminating dampness and a preparation method thereof. The traditional Chinese medicine is prepared from the following raw materials in parts by weight: radix sophorae flavescentis, mint, sanguisorba officinalis, rheum officinale, burdock, honeysuckle, oldenlandia diffusa, agastache rugosus and cortex dictamni. Furthermore, the invention discloses a preparation method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition disclosed by the invention, on an aspect of treating eczematous dermatitis, cutaneous pruritus and psoriasis, is concrete in curative effect, good in safety and free from side effect, and the composition is convenient to use, low in cost and better in effect compared with the prior art.

The present invention discloses crataegus pit extract or its medicinal composition and its application in preparation of medicine for curing or preventing mycetes, specially for resisting dermatomycosis and candidiosis. Said invention uses the crataegus pit as raw material, and adopts the existent technological process including the steps of pyrogenic distillation, purification and others to obtain the crataegus pit distillate oil raw liquor. Said medicine has no any toxic side effects, and its therapeutic effect is obvious.

The invention relates to primers and kit for fluorescent PCR detection of nine dermatophytes, and belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms. The primers include an upstream primer with a nucleotide sequence which is shown in SEQ ID No: 1 and a downstream primer with a nucleotide sequence which is shown in SEQ ID No: 2, wherein the nine dermatophytes include Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton schoenleinii, Epidermophyton floccosum, Trichophyta verruca, Microsporum canis and Microsporum gypsum. The detection is fast, sensitive, specific and repeatable through the primers, and the primers can be applied to early, non-invasive and accurate detection of clinical patients, so that assistance of clinical and effective antifungal therapy is achieved.

The invention belongs to the field of natural medicine and cosmetics, and provides application of Chinese torreya essential oil to preparation of anti-dermatophytes medicine, or application of Chinese torreya essential oil as efficiency ingredients in cosmetics for inhibiting the dermatophytes. In-vitro antibacterial activity study on the Chinese torreya essential oil shows that the Chinese torreya essential oil has better antibacterial activity on common pathogenic dermatophytes capable of causing tinea capitis, tinea corporis and little onychomycosis. The Chinese torreya essential oil is essential oil prepared from Chinese torreya aril by a distillation method, a cold squeezing method, a solvent extracting method or a carbon dioxide supercritical extraction method and the like. The Chinese torreya essential oil has the quality requirements that the Chinese torreya essential oil is colourless to yellowish Green liquid, and has the characteristic flavor of Chinese torreya fruit; the relative density (20 DEG C / 20 DEG C) is 0.850 to 0.860; the content sum of characteristic components of Alpha-pinene and limonene is greater than or equal to 30 percent. The invention discloses a new purpose of the Chinese torreya essential oil to prepare anti-dermatophytes medicine or cosmetics. In addition, an optimized preparation method of the Chinese torreya essential oil is provided by comparing the advantages and disadvantages of different extraction methods of the Chinese torreya essential oil.

The invention discloses a folium artemisiae argyi extract and a preparation method and application thereof to dermatophyte antibacterial agents, and belongs to the field of technical application of plant source antibacterial agents. The antibacterial agents are folium artemisiae argyi extracts prepared by taking traditional Chinese medicinal materials of folium artemisiae argyi as raw materials through ultrasonic extraction (5-75% ethanol) or ethanol reflux extraction, and have an efficient inhibition effect on dermatophytes such as trichophyton rubrum, trichophyton mentagrophytes and microsporum gypseum. After the folium artemisiae argyi extracts are separated and purified through macroporous adsorption resin, 40-95% ethanol separation components have different degrees of inhibition activity on the dermatophytes, wherein the 95% ethanol separation components are most significant. Meanwhile, the main chemical components in the 95% ethanol separation components are eupatilin, physcion, vitexin, brown cyanidin and the like. The folium artemisiae argyi extract can efficiently inhibit the dermatophytes, has the advantages of safety, high efficiency and low toxic and side effects, and provides a new drug source for prevention and treatment of dermatophyte diseases such as tinea of feet and hands.

An anti-Trichophyton film containing calcium hydrogen carbonate and an anti-Trichophyton forming agent enable easy formation, on a human skin, of an antibacterial film containing calcium hydrogen carbonate while assigning antibacterial effect possessed by a natural material thereto by using scallop shell as a raw material, and form an antibacterial film of calcium hydrogen carbonate with the antibacterial effect of the natural material added thereto through a simple structure, thereby enhancing the curative effect to athlete's foot. The anti-Trichophyton film forming agent is obtained by mixing a powder obtained by calcinating scallop shell mainly composed of calcium carbonate with distilled water. Remedies for dermatophytosis are prepared by grinding and calcinating scallop shells having a crystalline structure due to the calcite structure of calcium carbonate.

The invention relates to methods assessing nucleic acids encoding a dermatophyte extracellular serine / threonine-rich protein (ESTRP gene) in identifying and preferably differentiating between dermatophytes. The invention relates further to corresponding detection kits, in addition to isolated probes and oligonucleotides.

The invention discloses a jiumiao jiaxianling traditional Chinese medicine composition which is prepared from the following components in parts by weight: 1-30 parts of golden larch bark, 1-30 parts of common goldenrod, 1-30 parts of fructus cnidii, 1-30 parts of dragon blood, 1-30 parts of rheum officinale, 1-30 parts of golden cypress, 1-15 parts of indigo naturalis, 1-15 parts of dried alum and medical oil, including 0.1g-3 parts of artemisia scoparia oil, 0.1g-3 parts of clove oil, 0.1g-3 parts of zanthoxylum oil and 0.1g-3 parts of artemisia oil. The traditional Chinese medicine composition has the killing effect on dermatophytes, trichophyton rubrum, trichophyton gypseum, epidermophyton floccosum, alpha fungus, molds, saccharomycetes and the like and has the advantages that a patient feel less pain, the effect is good, the disease is not easy to relapse, the bacteria can be thoroughly killed, and the small toxic or side effect is caused.

A primer pair that includes a forward primer and a reverse primer is used to amplify a nucleic acid from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22. A nucleic acid capable of hybridizing specifically to a nucleic acid sequence from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22 is provided. A carrier that includes the nucleic acid is provided. A method can be used to detect in a sample a nucleic acid sequence including SEQ ID NO: 22 from a pathogen associated with a skin, hair, and nail infection. The primer pair, the nucleic acid, or the carrier may be useful for the diagnosis of a disease. A kit including the primer pair, the nucleic acid, and / or the carrier may be useful for the diagnosis of a disease.

Epidermophyton floccosum is one of the most common dermatophytosis pathogenic bacteria, and the invention discloses specific primer sequences for identifying the Epidermophyton floccosum. The Epidermophyton floccosum can be specifically identified by the pair of primer sequences through polymerase chain reaction (PCR) amplification, the speed and reliability are high, a theoretical basis is laid for diagnosing dermatophytosis of rabbit and purifying the dermatophytosis in rabbit farms, and technical guidance is provided.

The invention provides a medicine for treating skin itch dermatophytosis. The medicine is prepared from the following traditional Chinese medicinal materials in parts by weight: 1.5-2.0 parts of huechys, 1.5-2.0 parts of mylabris, 3.0-4.0 parts of brucea javanica kernel, 0.5-1.0 part of gamboge, and 300 parts of white vinegar. The medicine is simple in components for treating itching syndrome dermatophyte, low in price and good in curative effect, can be used for radically treating dermatophyte, is mainly used for treating psoriasis, tinea manuum, tinea pedis and the like, and solves the problems of complicated prescription, high cost, radical treatment unavailability and repeated attack and the like for current dermatophyte treatment.

The invention relates to an ointment for treating skin ringworm, which belongs to the technical field of western medicine; it consists of the following raw materials in proportions by weight: 1-30 parts of sulfur, 1-10 parts of salicylic acid, 1-10 parts of borneol, and 1-10 parts of benzoic acid 10 parts; the ointment of the present invention has the effects of sterilizing and reducing inflammation, relieving itching, and suppressing damp sores; the present invention has simple formula, low cost, wide application, quick effect, convenience and high cure rate.

The invention is to disclose a medicine of external use for treating skin disease And its preparation method, said medicine includes materials according to part by weight as follows: egocort 6-9 ; camphor 80-120;menthol 80-120;triamcinolone acetonaide acetate 8-12;carbamide 800-1200;aspirin 440-4400.

The invention discloses an artemisia apiacea pet shampoo formula and a production process thereof. The artemisia apiacea pet shampoo formula comprises the following main components in percentage by mass of 0.2 to 0.6 percent of artemisia apiacea oil, 10 to 18 percent of fatty alcohol-polyoxyethylene ether sodium sulfate, 3 to 5 percent of ammonium lauryl sulfate, 2 to 4 percent of an emulsifier, 2 to 4 percent of dodecyl dimethyl betaine, 3 to 5 percent of a thickening agent, 3 to 5 percent of a foam stabilizer, 0.4 to 0.6 percent of pearly concentrate, 0.2 to 0.4 percent of Casson preservatives, 1 to 3 percent of chlorhexidine gluconate and 55 to 65 percent of deionized water. 0.2-1% of artemisia apiacea oil is added into the pet shampoo, when the concentration of the artemisia apiacea oil is 0.25%, the pet shampoo has a bacteriostatic effect on all dermatophytes, when the concentration of the artemisia apiacea oil is 1%, the pet shampoo has a bactericidal effect on all dermatophytes, and the prepared shampoo is cool and mild and has the effects of clearing away heat and toxic materials and killing parasites to relieve itching.

The invention discloses a preparation method of a household test strip for rapidly detecting dermatophytes. The preparation method is mainly characterized by comprising the following steps: obtaining dermatophytes antigens; preparing a dermatophyte monoclonal antibody; preparing a colloidal gold reagent strip for antigen-antibody reaction; preparing a clinical sample treating fluid; and preparing a complete reagent strip set and an operation instruction. The method adopts the colloidal gold reagent strip to rapidly detect dermatophytes, is suitable for home use or grassroots units, people can check whether they are infected by dermatophytes such as dermatophytosis or onychomycosis by themselves, and then interrogates in internet hospitals or goes to drugstores or purchases antifungal drugs on the internet. The occurrence of misdiagnosis and mistreatment of superficial fungal infection and the economic and time cost of visiting a doctor in a hospital can be effectively reduced.

The invention discloses a herba speranskiae tuberculatae and cacumen platycladi mixed extract. The mixed extract comprises pinene, 2-carene, limonene, trans-caryophyllene, terpinyl acetate, phellandrene, palmitic acid, ethyl palmitate, 1-methoxy butane, 5,6,7,7A-tetrahydro-2(4H)-benzofuranone, 6,10,14-trimethyl-2-pentadecanone, methyl hexadecanoate and dibutyl phthalate. The herba speranskiae tuberculatae and cacumen platycladi mixed extract is mainly applicable to treatment of androgenetic alopecia and dermatomycosis such as tinea pedis, tinea manuum and tinea corporis. Herba speranskiae tuberculatae and cacumen platycladi are both clinical common medicines and are cheap, convenient to acquire, high in safety coefficient and available for oral administration and external application. Theextract is simple in use, high technical requirements on extraction are avoided, conventional traditional Chinese medicine impurities can be effectively removed, medicine purity is improved, extract effective components and reasonable compatibility are realized, a utilization process is simplified, and medicine quality uniformity is guaranteed.

The invention discloses a model for simulating dermatophyte infection in vitro and an establishment method and application thereof, and belongs to the technical field of bioengineering. The model forsimulating dermatophyte infection in vitro comprises matrix glue, an epidermal analogue embedded in the solidified matrix glue and dermatophyte hyphae, the epidermal analogue is of a relatively complete solid sphere structure, and the exterior of the epidermal analogue is a cell layer which is composed of substrate layer cells and substrate upper layer cells and distributed in a surrounding mode,and a keratinization sample non-cell layer is arranged inside; hyphae invade basal layer cells outside the sphere structure and do not invade or only invade a small amount of basal layer cells, and donot invade the keratinization sample non-cell layer inside the sphere structure; the model can effectively simulate the state that dermatophyte infects normal skin tissue, the infection process of the model can be directly observed under a phase contrast microscope, the infection situation can be detected through omics methods such as high-throughput RNA sequencing, and the model can be effectively used for researching the pathogenic mechanism of dermatophyte infection and screening anti-dermatophyte drugs.

The present invention provides an improved preparation based on the synergistic action of garlic extract and essential oil of M. spicata var. Ganga or cinnamon oil against dermatophytic fungus. More particularly, the present invention relates to the synergistic enhancement of activity of a combination by menthyl acetate or Geraniol. The invention also provides a method of preparation of the synergistic combination and the shelf life observed to be more than one year. The cream based preparation is a potent anti-dermatophytic as described and illustrated by in vitro and in vivo evaluations.

The invention belongs to the technical field of medicines, and particularly discloses a dryopteris fragrans compound Disaspidin BB and application of the dryopteris fragrans compound Disaspidin BB inthe antibacterial aspect. According to the invention, the phloroglucinol compound Disaspidin BB is separated and prepared from the dryopteris fragrans (L.) Schiott.), and the research results show that the compound has good antibacterial activity, especially has a very good antibacterial effect on dermatophyte and bacteria and is extracted from natural plants and has the characteristics of low toxic and side effects and low possibility of generating drug resistance and provides a new choice and way for developing antibacterial drugs and has a very good application prospect.

A primer pair that includes a forward primer and a reverse primer is used to amplify a nucleic acid from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22. A nucleic acid capable of hybridizing specifically to a nucleic acid sequence from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22 is provided. A carrier that includes the nucleic acid is provided. A method can be used to detect in a sample a nucleic acid sequence including SEQ ID NO: 22 from a pathogen associated with a skin, hair and nail infection. The primer pair, the nucleic acid, or the carrier may be useful for the diagnosis of a disease. A kit including the primer pair, the nucleic acid, and / or the carrier may be useful for the diagnosis of a disease.

The invention discloses a compound clobetasol propionate nano-medicament of which the particle size is 1-100 nanometers. The medicament consists of the following components in percentage by weight: 10.0-50 percent of surfactant, 0-8 percent of cosurfactant, 0.5-20 percent of oil, 0.001-4.0 percent of amitraz, 0.001-4.0 percent of clobetasol propionate, 0.001-10 percent of terbinafine and the balance of distilled water, wherein the total percentage by weight of the components is 100 percent. The medicament has the effects of resisting bacteria, diminishing inflammation, relieving itching and pain, killing mites and treating fungal diseases, eczema and various types of dermatitis caused by fungi such as dermatophyte, microsporosis capitis, acrothesium floccosum, microzyme of monilia and chaff ringworm fungus, and the like. The medicament is a yellow or colorless clear transparent liquid and has the advantages of high stability, high wetting property, good transdermal effect, nontoxicity, non-irritation, high safety, easiness for preparing, low cost and convenience for popularizing.

The invention relates to antifungal lipopeptid produced by microbial fermentation and an antifungal lipopeptid-containing skin liniment, belongs to the technical field of biology, and in particular relates to microbial bacillus subtilis BI1 with the preservation number of CGMCC No.3412 for producing the antifungal lipopeptid by using a fermentable sugar raw material. The invention also relates to an antifungal lipopeptid extract produced by microbial fermentation, and an extract-containing film coating agent and a preparation method thereof at the same time. When the film coating agent of the invention is used for treating dermatophytosis, the average cure time is 7 days and the cure rate reaches 100 percent, so a good therapeutic medicament for treating the dermatophytosis is provided for patients.

Dermatophytes which belong to one of the three genera Epidermophyton, Trichophyton and Microsporum are the main cause of fungal infections of skin, hair and nails. Traditional diagnostic procedures consist of microscopy and culture, but due to the slow growth rate of dermatophytes typically two to four weeks are needed before a final diagnosis is obtained. The present invention is a rapid DNA extraction method extracting nucleic acids from fungi (e.g. dermatophytes and other pathogenic fungi) which can be performed from directly on hair, nail or skin specimens from humans, from naturally or experimentally infected animals or from cultured fungal colonies for the use in PCR amplification and detection assays. The present invention also includes specific primer sets for detection of any dermatophyte and for species specific detection of Trichophyton rubrum and Epidermophyton floccosum by PCR and a kit for diagnosing fungal infections.