The invention discloses a detection method for activity of
telomerase. The method comprises the following steps: step 1, extracting
telomerase in to-be-detected cells by using the
CHAPS method; step 2, subjecting a captured substrate and the to-be-detected
telomerase to extension and subjecting an extension product and a reporter
label solution to a
hybridization reaction, wherein the captured substrate is prepared by connecting a telomerase substrate to the surface of a gold shell-coated
ferriferrous oxide nanoparticle, and the reporter
label solution is prepared by connecting a
telomere complementary sequence and a Raman molecule to the surface of a spherical gold
nanoparticle; and step 3, observing the color of a
hybridization reaction product by using colourimetry or carrying out dual
signal path detection by using SERS technology so as to obtain SERS signals. According to the invention, colourimetry and SERS technology are integrated into a same telomerase
activity detection system, colourimetry is used to realize rapid
qualitative analysis of a sample, then the SERS technology is used to realize accurate quantitative analysis of the sample, and combination of the two methods enables rapid high-sensitivity telomerase
activity detection to be realized.