Electrochemical sensor for detecting telomerase activity and method for manufacturing electrochemical sensor

Abstract

The invention discloses an electrochemical sensor for detecting telomerase activity and a method for manufacturing the electrochemical sensor. The method for manufacturing the electrochemical sensor mainly includes procedures of sequentially adsorbing DNA (deoxyribonucleic acid) strands S1 of thionine, Au@SiO<2> and G-rich structures in the surfaces of molybdenum disulfide nano-sheets and adding Hemin into the molybdenum disulfide nano-sheets to form MoS<2>-Thi-Au@Si<O>2 / DNAzyme nano-composite materials; modifying TS (telomerase substrate) precursors on the surfaces of gold electrodes, extending the DNA strands by the aid of telomerase to form DAN strands S2, screwing the DNA strands S2 and the DNA strands S1 complementarily, and ultimately detecting the intensity of electrochemical signals of catalytic hydrogen peroxide oxidation ABTS to directly detect the telomerase activity. The electrochemical sensor and the method have the advantages that the electrochemical sensor manufactured by the aid of the method is simple in operation and high in sensitivity and stability and has an important significance on detecting cancerous cells.

Description

technical field [0001] The invention relates to an electrochemical sensor for detecting telomerase activity and a preparation method thereof, in particular to a G-quadruplex / DNAzyme-based electrochemical sensor for detecting telomerase activity and a preparation method thereof, belonging to The field of biological detection technology. Background technique [0002] At present, the incidence of cancer is increasing year by year due to various factors such as the aging of the population, the destruction of the ecological environment, and unhealthy lifestyles. However, the five-year survival rate of cancer patients in my country is only about 30%. The most fundamental reason is When the patient is diagnosed with cancer, he is already in the middle and late stage, thus delaying the best treatment time. Therefore, rapid and effective early and sensitive diagnosis is of great significance for timely detection of cell canceration. [0003] The role of telomerase in the process of ...

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Problems solved by technology

At present, the most commonly used method for detecting telomerase activity is the TRAP method based on PCR amplification technology. The invention patent "a method for detecting telomerase activity" (patent No. 02116466.5) is based on PCR technology and reverse transcription of human telomerase The detection of a certain structural domain of the enzyme is used to determine the activity of telomerase, the detection signal is stable, and a large number of samples can be reproducibly operated at the same time, but the process is complicated and the sensitivity is low; the invention patent "a rapid detection of telomerase activity Kit and its application” (Patent No. 201210190716.3) uses the property that exonuclease III can gradually remove single nucleotides at the 3’ blunt end or 3’ indented end of double-stranded DNA to measure the activity of telomerase. It avoids the defect that PCR technology is prone to false positive or false negative results, but the requirement for telomerase concentration is relatively high, and it is impossible to detect a very small amount of cancer cells with telomerase activity

[0004] At present, the diagnosis of cancer in our country is mainly realized through laboratory immunology, enzyme detection, imaging detection, etc., but the high cost of examination makes it impossible to popularize in the general health examination process

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