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Telomerase activity detection kit and detection method thereof

A technology for activity detection and telomerase, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of limited application, difficulty in obtaining satisfactory sensitivity and detection range, and achieve improved detection sensitivity and high Sensitivity and detection range, the effect of inhibiting non-specific amplification

Inactive Publication Date: 2013-02-13
XI AN JIAOTONG UNIV
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Problems solved by technology

Although these methods are simpler and faster, it is difficult to obtain satisfactory sensitivity and detection range. At the same time, some expensive or difficult-to-synthesize materials also limit the practical application of this type of method.

Method used

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  • Telomerase activity detection kit and detection method thereof
  • Telomerase activity detection kit and detection method thereof
  • Telomerase activity detection kit and detection method thereof

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Embodiment Construction

[0076] The present invention will be further described in detail below with reference to specific drawings and specific embodiments, which is an explanation rather than a limitation of the present invention.

[0077] See figure 1 , A schematic diagram of the detection of telomerase activity based on the ternary connection structure and base stacking-mediated isothermal cascade signal amplification, where a) is the reaction process of telomerase extending the substrate, b) and c) are based on DNA molecular machines Isothermal cascade signal amplification process.

[0078] Specifically used 6 kinds of oligonucleotide probes, including telomerase substrate, 3WJ template, 3WJ primer, primer1, primer2, and molecular beacon probe; their functions are described as follows:

[0079] Telomerase substrate, which can be recognized by telomerase and undergo an elongation reaction; after it is combined with telomerase, it uses the reverse transcription activity of telomerase to extend the telomer...

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Abstract

The present invention discloses a telomerase activity detection kit and a detection method thereof. According to the kit, a ternary linking structure probe is adopted to recognize a telomerase product, and a ternary linking structure triggered DNA molecule machine and a base stacking triggered DNA molecule machine are combined to achieve isothermal cascade nucleic acid amplification to convert a reverse transcription event of the telomerase in a tumor cell extract into a significantly-enlarged fluorescence detection signal so as to precisely determine telomerase activity in a trace amount of tumor cells and achieve detection on tumor cell telomerase activity inhibition by chemical drugs.

Description

Technical field [0001] The invention belongs to the technical field of telomerase activity detection, and relates to a telomerase activity detection kit and a detection method thereof. Background technique [0002] Telomerase (Telomerase) is a nuclear protein reverse transcriptase, composed of RNA templates and protein catalytic subunits. Through reverse transcription, it can extend the repetitive sequence (TTAGGG) n at the 3'end of chromosome telomeres, thereby stabilizing the length of telomeres and inhibiting cell division. In normal human tissues and cells, the activity of telomerase is inhibited. In more than 85% of known tumor cells, telomerase often has a higher activity, which is considered to be related to the activation of tumor cells, transformation and proliferation. closely related. In the field of clinical diagnosis, telomerase is an important malignant tumor marker. In addition, many cancer treatment methods and drugs also target the inhibition of telomerase act...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/48
Inventor 赵永席齐林陈锋赵越张青
Owner XI AN JIAOTONG UNIV
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