High-efficiency rapid bladder cancer urinalysis kit and urinary cell active preservative fluid

Abstract

The invention relates to a high-efficiency rapid bladder cancer urinalysis kit and a urinary cell active preservative fluid, which belong to the field of biomedicine. The high-efficiency rapid bladder cancer urinalysis kit contains the urinary cell active preservative fluid and a specific fluorescence labeled primer for detecting the activity of telomerase. The kit can perform detection of the activity of the telomerase on cells taken from urine further to efficiently and accurately detect whether a patient suffers from diseases related to the activity of the telomerase such as bladder cancer; the urinary preservative fluid in the kit is a key factor; the activity of the telomerase of an early cancer patient has lower expression level, the activity of the telomerase is difficult to detect in a small amount of tested cells, and the urinary preservative fluid can ensure that collected urinary cells preserve good activity so as to provide adequate time for acquiring adequate active cells; and the high-efficiency rapid bladder cancer urinalysis kit and the urinary cell active preservative fluid provide a feasible way for accurately detecting early bladder cancer and non-invasively monitoring the bladder cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an efficient and rapid bladder cancer urine test kit and urine cell activity preservation solution. Background technique [0002] Urine samples contain physiological metabolic waste substances, including organic and inorganic components and electrolyte components containing trace elements. Some urine will contain proteins and carbohydrates, which are pathological metabolites; A series of tissues such as the bladder, therefore, urine also contains a small amount of tissue exfoliated cells. In view of the close relationship between urine and human metabolism, it is used in many analytical experiments or clinical tests. However, it is precisely because of the rich components in the urine sample that the bacteria can rapidly multiply in it. When the urine sample cannot enter the detection procedure in time, the bacteria and some of the components will inactivate the components that reflect...

AI Technical Summary

Problems solved by technology

Combined with the method of adding internal standards, such as the patent application with the patent number 00111629.0 and the name "Quantitative Assay Method for Telomerase Activity", the technical solution provides very ideal primers, internal reference primers and internal standard templates, which can well avoid Due to defects such as false negative results and non-specific amplification caused by primer dimers, since the fluorescent quantitative PCR technology had not been developed at that time, it was impossible to accurately and quantitatively detect the substrate, but combined with modern PCR fluorescent technology, it will be able to detect sensitively and specifically Quantification of telomerase activity in cells

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