163 results about "Cytokine synthesis" patented technology

The methods and compositions provided herein relate generally to IL-10 specific antibodies and uses thereof. More specifically, compositions of humanized IL-10 specific antibodies and methods to use such antibodies in modulating the biological activity of IL-10, particularly in autoimmune disorders and pathogen-mediated immunopathology.

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selective for the N-terminus on one subunit of IL-10 with little or no formation of monomeric IL-10. The method also provides a substantially homogenous population of mono-PEG-IL-10.

The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more markers selected from the group consisting of Cytoplasmic aspartate aminotransferase, soluble Tumor necrosis factor receptor superfamily member 5, soluble CD40 Ligand, soluble C-X-C Motif chemokine 16, S100-A12, Eotaxin, soluble E-selectin, Fibronectin, Granulocyte colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor, Heparin-binding growth factor 2, soluble Hepatocyte growth factor receptor, Interleukin-1 receptor antagonist, Interleukin-1 beta, Interleukin-10, Interleukin-15, Interleukin-3, Myeloperoxidase, Nidogen-1, soluble Oxidized low-density lipoprotein receptor 1, Pappalysin-1, soluble P-selectin glycoprotein ligand 1, Antileukoproteinase, soluble Kit ligand, Tissue inhibitor of metalloproteinase 1, Tissue inhibitor of metalloproteinase 2, soluble Tumor necrosis factor, soluble Vascular cell adhesion molecule 1, and Vascular endothelial growth factor A as diagnostic and prognostic biomarkers in renal injuries.

The present invention relates to a phenotypically distinct CD1dhigh CD5+ B cell subset that regulates T cell mediated inflammatory responses through the secretion of interleukin-10 (IL-IO). The invention also relates to the use of these IL-IO producing regulatory B cells in the manipulation of immune and inflammatory responses, and in the treatment of disease. Therapeutic approaches involving adoptive transfer of these regulatory B cells, or expansion of their endogenous levels for controlling autoimmune or inflammatory diseases and conditions are described. Ablation of this subset of regulatory B cells, or inhibition of their IL-IO production can be used to upregulate immunodeficient conditions, and / or to treat tumors / cancer. Diagnostic applications also are encompassed.

A mathematical prognostic in which changes in a number of physiologically significant factors are measured and interpolated to determine a "damage function" incident to bacterial infection or other serious inflammation. By measuring a large number of physiologically significant factors including, but not limited to, Interleukin 6 (IL6), Interleukin 10 (IL10), Nitric Oxide (NO), and others, it is possible to predict life versus death by the damage function, dD / dt, which measures and interpolates differential data for a plurality of factors. In mammals, an IL6 / NO ratio <8 at 12 hours post infection is highly predictive (60%) of mortality; also in mammals, an IL6 / NO ratio <4 at 24 hours post infection is highly predictive (52%) of mortality; and an IL6 / IL10 ratio in mammals of <7.5 at 24 hours post infection is highly predictive (68%) of mortality.

An adoptive immunotherapy using ex vivo-generated regulatory T cells may be used for the suppression of undesireable immune response. T cells are to be obtained from the patient's blood, and upon exposure to a set of toxins from the pathogen A. actinomycetemcomitans, the population of regulatory T cells will be enriched ex vivo and adoptively transferred back to the patient. The novel aspect of the present invention is that it generates large numbers of type 1 regulatory T cells, which secrete Interleukin-10.

The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more markers selected from the group consisting of Cytoplasmic aspartate aminotransferase, soluble Tumor necrosis factor receptor superfamily member 5, soluble CD40 Ligand, soluble C-X-C Motif chemokine 16, S100-A12, Eotaxin, soluble E-selectin, Fibronectin, Granulocyte colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor, Heparin-binding growth factor 2, soluble Hepatocyte growth factor receptor, Interleukin-1 receptor antagonist, Interleukin-1 beta, Interleukin-10, Interleukin-15, Interleukin-3, Myeloperoxidase, Nidogen-1, soluble Oxidized low-density lipoprotein receptor 1, Pappalysin-1, soluble P-selectin glycoprotein ligand 1, Antileukoproteinase, soluble Kit ligand, Tissue inhibitor of metalloproteinase 1, Tissue inhibitor of metalloproteinase 2, soluble Tumor necrosis factor, soluble Vascular cell adhesion molecule 1, and Vascular endothelial growth factor A as diagnostic and prognostic biomarkers in renal injuries.

The invention discloses a fusion protein with transdermal capability and interleukin 10 activity as well as a coding gene and application thereof. The fusion protein provided by the invention is formed by connecting any one protein of (1) human IgG Fc-gamma1 or the mutain thereof and (2) human IgG Fc-gamma2 or the mutain thereof on the carboxyl terminal of a human interleukin 10 protein, and connecting a transit peptide on an amino terminal; wherein the amino acid sequence of the human interleukin 10 protein is the amino acid sequence from the 12th site to the 171st site on the amino terminal of a sequence 2 in a sequence table; and the amino acid sequence of the transit peptide is the amino acid sequence from the 1st site to the 11th site on the amino terminal of the sequence 2 in the sequence table. Tests prove that the fusion protein provided in the invention has the transdermal capability and the interleukin 10 activity simultaneously, and has greater practical significance and broad application prospect in the field of the preparation of medicaments for treating autoimmunity reaction and especially psoriasis.

Polypeptides comprising at least a first and second Fc fragment of IgG that can be used to induce a stimulated cell to produce the anti-inflammatory cytokine Interleukin-10 and methods of using the same are disclosed herein.

The invention relates to the field of genetic engineering medicines, in particular to a human interleukin 10-Fc (IL10-Fc) fusion protein, and a coding gene and application thereof. By replacing aminoacids at multiple positions in an IgG4Fc part, the modified IL10-Fc fusion protein has superior performance over existing Fc fusion proteins, such as increasement of in-vivo stability, elimination ofunnecessary effector functions and reduction of immunogenicity of the fusion proteins in living organisms. The invention also discloses a method for treating diseases by using an IL10-Fc fusion protein medicament, and the method comprises administering a therapeutically effective amount of the medicament to a subject suffering from diseases, wherein the diseases comprise an inflammatory symptom, immune related diseases, fibrosis diseases, cancers and the like.

The present invention provides compositions and methods for upregulating IL-10 production in a stimulated cell. In one aspect, the invention provides methods of identifying ERK activating agents capable of activating and amplifying the ERK MAPK pathway in a cell. Such ERK activating agents are capable of upregulating the production of IL-10 in stimulated cells. In another aspect, the invention provides ERK activating agents identified by the screening methods of the invention. Methods are also provided for preventing and treating inflammation in a susceptible patient by administering to the patient, a therapeutically effective amount of an ERK activating agent identified in accordance with the invention.

Described herein are methods of reducing Salmonella in the intestines of poultry in need thereof by administering to a poultry bird an effective amount of an interleukin-10 peptide or an isolated antibody that specifically binds an interleukin-10 peptide. Administering may be performed within 1 to 4 weeks of harvest of the poultry in order to reduce Salmonella transmission to human consumers. Also included herein are finishing feeds that include an interleukin-10 peptide or an isolated antibody that specifically binds an interleukin-10 peptide.

The invention discloses a liquid band-aid, comprising, active materials, a film-forming material and a solvent. The active materials include cytokines 0.042 to 0.0095%, which are chosen from at least two of EGF (epidermal growth factor), PDGF (platelet derived growth factor), FGF (fibroblast growth factor) and IL-10 (interleukin-10). The film-forming material comprises chitosan hydrochloride 2% to 8%. The solvent is deionized water or distilled water. The invention further discloses a method for preparing the liquid band-aid. By the use of the preparation method and / or the liquid band-aid comprising various biological active materials and prepared by the preparation method, activity of the biological active materials can be retained, and wounds can be efficiently repaired without a scar.

The present disclosure is directed to interleukin-10 (IL-10) peptides and isolated antibodies that specifically bind to the IL-10 peptides. The IL-10 peptides and the isolated antibodies may be administered alone or as an animal feed additive to treat gastrointestinal protozoan infection in animals.