35 results about "CA19-9" patented technology

The invention relates to a magnetic corpuscule chemiluminescent enzyme immunoassay kit for detecting carbohydrate antigen and the application method thereof. The kit comprises FITC antibody-coated magnetic corpuscules; a marker solution prepared by mixing the FITC-marked carbohydrate antigen monoclonal antibody and the enzyme-marked carbohydrate antigen monoclonal antibody; a carbohydrate antigen standard sample solution; a concentrated washing solution; and a luminescent substrate solution, wherein carbohydrate antigen optionally adopts one of CA72-4, CA50, CA19-9, CA242, CA15-3, CA27-29 and CA125. The enzyme-marked antibody and the FITC-marked antibody are the monoclonal antibodies corresponding to the antigens. The FITC antibody coating the magnetic corpuscules adopts a polyclonal antibody or a monoclonal antibody. The marker solution is prepared by mixing an FITC-marked capture antibody working solution and an enzyme-marked antibody pair working solution by the volume ratio of 1:(1-3). Compared with the known kit for mensurating the carbohydrate antigen, the kit has the advantages of high flux, high sensitivity, wide linear range, rapidness, etc., and has a wide application prospect for the clinical inspection, etc.

The invention discloses a male multi-tumor marker detection protein chip and a kit thereof. The chip comprises a substrate, protein markers distributed in an array type and point coatings of contrasts, wherein the substrate is a glass substrate or a film substrate; and the tumor markers and the point coatings of the contrasts are seven protein markers of AFP, CEA, NSE, CYFRA21-1, CA19-9, tPSA and SCC-ag, a positive contrast and a negative contrast which are uniformly distributed and latticed on the substrate. A reaction result of various indexes can be obtained only through one reaction by utilizing the protein chip, and the following tumors can be simultaneously screened: primary liver cancer, prostatic cancer, pancreatic cancer, lung cancer, esophageal cancer, gastric cancer and colorectal cancer. The invention is particularly suitable for the general examination of malignant tumors of male asymptomatic groups and high risk groups.

UL16 binding protein 2 (ULBP2) is a protein overexpressed in pancreatic cancer tissues, and the ULBP2 levels are significantly higher in pancreatic cancer patients than those in healthy controls. This invention provides a method to detect pancreatic cancer using ULBP2 as a serological marker. The combination of ULBP2 and CA19-9 promotes the efficacy of pancreatic cancer detection. When measuring the blood ULBP2 levels in patients with other cancer types, including colorectal carcinoma, nasopharyngeal carcinoma and gastric cancer illustrates the blood ULBP2 levels are higher in patients with pancreatic cancer than other cancer types.

The invention discloses a pancreatic ductal adenocarcinoma marker and a screening method thereof, which belongs to the field of clinical test and diagnosis. In view of the problem that the detection sensitivity and the detection specificity of the existing pancreatic ductal adenocarcinoma diagnosis marker are poor, serum of an early patient of early stage pancreatic duct adenocarcinoma is subjected to a trace metabolomics analysis by the high-performance liquid chromatography-tandem mass spectrometry technology, and different metabolites between normal human and the early stage pancreatic ductal adenocarcinoma patients are found. The different metabolites between normal human and pancreatic ductal adenocarcinoma patients are further analyzed by this technique to find the specific differentmetabolites C10:1 acyl carnitine and lysophosphatidyl choline LysoPC (14:0) of pancreatic ductal adenocarcinoma patients caused by cancer, i.e., the diagnostic molecules of the pancreatic ductal adenocarcinoma. According to the pancreatic ductal adenocarcinoma marker and the screening method thereof, the method can assist CA19-9 in diagnosing pancreatic duct adenocarcinoma patients, and can improve the diagnosis rate for the CA19-9 negative patients by 85%. The method is suitable for the screening of tumor markers.

The invention discloses an application of a Glypican-1 protein in the diagnosis of pancreatic cancer, a detection method of a Glypican-1 positive exosome concentration, and a use of the detection method. A nano-plasma enhanced scattering (nPES) detection technology can be used to quantify exosomes in serum, an exosome extracting process is omitted, the characteristic antigen of the exosomes is used as a target, and the antigen and a corresponding antibody carrying gold nanoparticles form an antigen-antibody complex in order to obtain exosomes, and the amount of the exosomes is reflected by using the light radiation principle of the gold nanoparticles. The Glypican-1 is a pancreatic cancer-derived exosome biomarker, and the content of Glypican-1 positive exosomes in blood plasma has specific specificity even in early pancreatic cancer, so the exosome content index can be used to diagnose the pancreatic cancer, and the exosome level is closely related to the staging and progression of pancreatic cancer patients. Experiments prove that the method using the nPES detection technology to detect the content of the Glypican-1 exosomes in the serum is concise and accurate, and has better sensitivity and specificity than CA19-9 in the diagnosis of the pancreatic cancer.

The present invention provides a tumor screening marker that can be actually used in clinical practice to detect colorectal cancer, and a tumor progression marker that can complement CEA or CA19-9. Galectin-1 used as a tumor screening marker or a tumor progression marker for colorectal cancer. Galectin-3 used as a tumor screening marker. Galectin-4 used as a tumor progression marker, a tumor screening marker, or a prognostic prediction marker for colorectal cancer. A method of analyzing the galectin concentration in a collected blood sample using the galectin. A colorectal cancer marker detection kit comprising a detection antibody selected from the group consisting of a fluorescently labeled galectin-1 antibody, a fluorescently labeled galectin-3 antibody, and a fluorescently labeled galectin-4 antibody.

The invention discloses a kit for dry-type fluorescent quantum dot joint detection diagnosis of pancreatic cancer and a preparation method of the kit. The kit can be used for joint detection of three tumor markers Ca19-9, CEA, CA242 for clinical diagnosis of pancreatic cancer on the same detection strip, monoclonal antibodies of CA19-9, CEA and CA242 are fixed on a nitrocellulose film by means of an immunochromatography technique for preparation of a reaction film, the three monoclonal antibodies are marked with quantum dots emitting different light, the antibodies marked with the quantum dots are mixed and sprayed on a glass fiber film for preparation of a conjugate pad, and the conjugate pad, a sample pad and a water absorbing pad are combined to form a quantum dot immunostrip. The kit is prepared with a double-antibody sandwich method, three antigens in whole blood or serum and plasma are quickly detected, numerous defects of existing independent detection methods are avoided, the three tumor markers can be detected simultaneously with one drop of blood, the result is more direct, the sensitivity and the specificity are both remarkably improved, and accordingly, the kit has great potential in clinical application.

The invention provides a colloidal gold immunochromatographic test strip for aided detection of pancreatic cancer and a preparation method of the golloidal gold immunochromatographic test strip. The strip comprises a bottom plate, a sample absorbent pad, a colloidal gold conjugate pad, a nitrocellulose membrane and a water absorption pad; when the strip is prepared, the sample absorbent pad, the colloidal gold conjugate pad, the nitrocellulose membrane and the water absorption pad are sequentially adhered to the bottom plate in an overlapped manner; the colloidal gold conjugate pad is coated with colloidal gold-labeled CA19-9 monoclonal antibody and colloidal gold-labeled monoclonal antibody of helicobacter pylori hemagglutinin antigen; the nitrocellulose membrane is coated with a detection line 1, a detection line 2 and a quality control line; the detection line 1 is coated with colloidal gold-labeled CA19-9 monoclonal antibody; the detection line 2 1 is coated with colloidal gold-labeled monoclonal antibody of helicobacter pylori hemagglutinin antigen; the quality control line is coated with goat anti-rabbit IgG antibody. The strip is simple in preparation and operation steps and high in sensitivity, and provides a good basis for early examination of pancreatic cancer.

The invention provides an early diagnosis method of liver cancer for a subject, comprising the steps of: 1) obtaining a biological sample from the subject; 2) detecting the content of a plurality of biomarkers in the biological sample, wherein the plurality of biomarkers are selected from two or more of AFP, AFP-L3, GP73, Fuc-GP73, DCP, CEA, CA19-9, TK1, DKK1, [Gamma]-GT, ALP, AFU, ALT and Fuc-Kininogen 1; 3) calculating the probability that the subject has liver cancer based on the detection result of step 2); and 4) comparing the probability with a set threshold, and when the probability isgreater than or equal to the set threshold, the subject is considered to have liver cancer; when the probability is less than the set threshold, the subject is considered not to have liver cancer. Theinvention also provides a corresponding detection kit and a detection device. The early diagnosis method and the diagnostic kit of liver cancer improve the sensitivity and the specificity of early diagnosis of liver cancer by the combined detection of various markers, and provide a more reliable basis for clinical diagnosis of liver cancer.

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and a preparation method thereof. A coating film, a magnet particle pad combined with CA19-9 antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A CA19-9 antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology and the biotin-avidin system into the quantitative detection of CA19-9 in blood, and has the advantages that the detection sensitivity and the reliability are greatly improved, the detection is accurate and rapid, long-time waiting is not required, the cost is low, the operation method is simple and convenient and the detection can be controlled easily.

The invention provides a pancreatic cancer protein biomarker detection kit including a CRP monoclonal antibody, an ICAM-1 monoclonal antibody, an OPG monoclonal antibody, a CA19-9 monoclonal antibody, and an enzyme-labelled secondary antibody for forming double antibody sandwich. The invention also provides a pancreatic cancer detection system comprising the detection kit. According to a detection chip and the detection kit, four pancreatic cancer protein biomarkers of CRP, ICAM-1, OPG and CA19-9 obtained through screening by means of 'subtraction method' are subjected to immunization to respectively prepare the specific CRP monoclonal antibody,the specific ICAM-1 monoclonal antibody, the specific OPG monoclonal antibody and the specific CA19-9 monoclonal antibody to be used as the basis, and the enzyme-labelled secondary antibody with double antibody sandwich is combined, and through antigen-antibody specific combination and combination of the enzyme-labelled secondary antibody with double antibody sandwich, biomarking proteins are detected, and higher accuracy and specificity are achieved.

The invention discloses a gene chip for detecting pancreatic cancer, comprising a solid phase vector and a probe, wherein the probe is hybridized with nucleotide sequences of related pancreatic cancer genes to be detected and/or complementary sequences of the nucleotide sequences; the related pancreatic cancer genes to be detected comprise P21, smad4, P16, c-Myc, bc1-2, Fas, CA19-9, SERPINB9, RHOT1 and P53 gene. The invention further discloses a method for detecting the pancreatic cancer by applying the gene chip. The gene chip of the invention can be used for performing effective detection at the early period of generation and development of the pancreatic cancer so as to improve cure rate and reduce mortality rate.

The invention belongs to the technical field of molecular biology and clinical detection, and relates to a protein combination, wherein the protein combination is selected from any two or three or four or five or six of CA19-9, CEA, HGF, IP-10, MIP-1beta and SDF-1alpha. The invention further relates to a ligand combination specifically bound with proteins in the protein combination, and application of the protein combination or the ligand combination to preparation of a kit for colorectal cancer screening, auxiliary diagnosis, treatment monitoring and prognosis judging. A serum protein markercombination can be used for colorectal cancer screening, auxiliary diagnosis, treatment monitoring and prognosis judging and particularly used for early colorectal cancer screening and auxiliary diagnosis, and the sensitivity of the serum protein marker combination is superior to that of a single protein marker and commonly used clinical serum markers CA19-9 and CEA in detecting.