CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit

A diagnostic kit, breast cancer technology, applied in the field of biomedicine, can solve the problems of difficulty in detecting early or recurrent breast cancer patients, inability to provide, low detection sensitivity and specificity, etc.

Active Publication Date: 2012-09-19
BEIJING MOKOBIO LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the single detection of one of the above tumor markers cannot provide more information, and the detection sensitivity and specificity are relatively low. It is difficult to detect patients with early or recurrent breast cancer, and misdiagnosis or missed diagnosis is prone to occur.

Method used

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  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit
  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit
  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit

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Embodiment 1

[0030] Example 1 Preparation of the colloidal gold five-inspection diagnostic kit of the present invention

[0031] 1. Preparation of monoclonal antibodies against CA15-3, CEA, CA19-9, CA12-5, SF

[0032] CA15-3, CEA, CA19-9, CA12-5, SF (CEA antibody purchased from Tianjin Tianjian Biopharmaceutical Co., Ltd., SF antibody purchased from Medix Biochemica, CA125, CA153, CA199 purchased from CanAg Diagnostics AB) / c Pure line mice are immunized, and when the mouse serum produces the corresponding antibody, the spleen cells can be fused with SP2 / 0 cells. Hybridoma cells were selected by HAT, and McAb was detected by enzyme-linked immunosorbent assay (ELISA). For hybridomas with positive detection antibodies, the limiting dilution method should be used for cloning, while the positive cell lines should be preserved, and a large number of monoclonal antibodies can be prepared using BALB / c pure-line mice. At the same time, antibody specific identification, identification of epitope recogn...

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Abstract

The invention discloses a diagnosis kit for breast cancer and preparation method thereof. Five kinds of tumor markers for diagnosis of breast cancer such as CA15-3, CEA, CA19-9, CA12-5, SF are detected together at the same test paper and using immunity chromatography technique the monoclonal antibodies for CA15-3, CEA, CA19-9, CA12-5, SF are fixed on the cellulose nitrate film, at the same time the five kinds of monoclonal antibodies are marked by gold particles of different size and the said gold antibodies are mixed to produce gold affixture pad, finally the gold affixture pad is assembled with a sample treating pad to form a test paper. The said kit quickly test the five kinds of antigens in human whole blood or serum, plasm using double antibody sandwiching method, so as to conquer many defects of previously separation detecting method, only one drop of blood can detect five tumor markers and the detection result is more direct and the sensitivity and specificity of detection is greatly increased.

Description

Technical field [0001] The invention relates to a kit for diagnosing tumors, in particular to a breast cancer colloidal gold five-inspection diagnostic kit. The invention also relates to a preparation method of the kit and its application in diagnosing breast cancer, belonging to biomedicine field. Background technique [0002] Breast cancer is the most common epithelial cell tumor in the world, posing a major threat to human health and survival, and is one of the most important social problems faced by countries all over the world. Today, breast cancer accounts for 7%-10% of systemic malignant tumors, and its incidence has been increasing year by year, and has been increasing at a rate of 3% every year since 1980. This is especially true in developed countries such as North America and Western Europe. About 1.2 million women worldwide develop breast cancer each year, and 500,000 women die of breast cancer. 2006 American Cancer Society; International Cancer Society; Internatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/558
Inventor 金鑫戴路
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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