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Carbohydrate antigen 19-9 time-resolved immunofluorescence assay and kit

A carbohydrate antigen and immunofluorescence technology, applied in analytical materials, measuring devices, instruments, etc., can solve problems such as insufficient accuracy, and achieve the effects of small cross-reaction, short detection time, and high analytical sensitivity.

Active Publication Date: 2015-08-26
瑞孚迪生物医学(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the method of the present invention, the technical problem in the prior art about the insufficient accuracy of CA 19-9 detection of national quality control serum can be solved

Method used

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  • Carbohydrate antigen 19-9 time-resolved immunofluorescence assay and kit
  • Carbohydrate antigen 19-9 time-resolved immunofluorescence assay and kit
  • Carbohydrate antigen 19-9 time-resolved immunofluorescence assay and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Example: Determination of Carbohydrate Antigen 19-9 (CA 19-9)

[0039] 1. Preparation of standard products

[0040] With 20% (v / v) calf serum, 0.1% NaN 3 (w / v) normal saline was prepared from carbohydrate antigen 19-9 purchased from Hytest Company into 0, 15, 50, 100, 200, 500 U / ml standard solutions. Aliquot and store at 2-8°C.

[0041] 2. Preparation of coated microporous reaction strips

[0042](1) Dilute the purified anti-carbohydrate antigen 19-9 monoclonal antibody (purchased from Hystest Company) to 1-10 μg / ml with 50 mM carbonate buffer solution of pH 9.3-9.7 (in this embodiment, 4 μg / ml was used) / ml), and then add 100 μl / well into each well of the microwell reaction strip, and let it stand for 5 to 24 hours;

[0043] (2) After washing the antibody-coated microwell reaction strip prepared in step (1) with a buffer solution containing a surfactant, add a buffer solution containing blocking protein, and let stand for 3 to 12 hours to block;

[0044] Wherein,...

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Abstract

The invention discloses a time resolution immunofluorescence analyzing method and a kit for saccharides antigen 19-9. The analyzing method comprises the following steps of: 1, preparing a solid-phase antibody; 2, preparing a lanthanide ion labeled antibody; and 3, adding a standard product or a sample to be measured of the saccharides antigen 19-9 into a solid-phase material with a solid-phase antibody prepared in the step 1, performing reaction on a buffer liquid, and after the solid-phase material is incubated and washed, adding the diluted labeled antibody and incubating again to perform fluorescence detection. The invention also provides the corresponding kit. The method and the kit have the characteristics of high sensitivity, high specificity and high stability, short detection time and high coincidence rate of detecting national quality control products of CA19-9.

Description

technical field [0001] The present invention relates to a time-resolved immunofluorescence analysis method and a kit, in particular to a time-resolved immunofluorescence analysis method and a kit for carbohydrate antigen 19-9, and involves the use of mixed labeled antibodies to resolve the carbohydrate antigen 19-9 ( CA19-9) The compliance rate of national quality control products. Background technique [0002] CA19-9 is a digestive tract tumor-associated carbohydrate antigen, secreted by gastrointestinal epithelial mucosal cells, with a molecular weight of about 5000KD, and its structure is a combination of Lea blood group antigen and sialic acid Lexa. High diagnostic value, especially for pancreatic cancer, gallbladder bile duct cancer and liver cancer; the positive rate of pancreatic cancer can be as high as 85%-95%. CA19-9 is often used for follow-up monitoring after treatment. When CA19-9 continues to increase, it indicates that there may be residual tumor or metastasi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 于春红夏秋兰
Owner 瑞孚迪生物医学(上海)有限公司
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