Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and preparation method thereof

A tumor-related antigen, magnetic immunochromatography technology, applied in the field of medical testing, can solve the problems of high subjectivity, deviation of results, environmental pollution, etc., and achieve the effect of simplifying the preparation process and easy operation

Inactive Publication Date: 2010-06-30
CHEMCLIN DIAGNOSTICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current diagnostic techniques for CA19-9 in blood mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence (CLIA), time-resolved fluorescence and other methods, but these methods have their own characteristics and shortcomings : Radioimmunity is a commonly used method in clinical practice. Its advantages are relatively accurate results and a wide linear range. The disadvantages are that the operation is cumbersome and time-consuming, and radioactive markers will cause harm to the operator and cause environmental pollution. At present, it has been gradually adopted. replaced by other methods
Its appearance greatly simplifies people's detection behavior. However, due to the influence of various factors, immunochromatography technology can only achieve qualitative detection and semi-quantitative or inaccurate quantitative detection; the detection sensitivity of immunochromatography also needs to be improved. In

Method used

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  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and preparation method thereof
  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and preparation method thereof

Examples

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Embodiment 1

[0033] Preparation method of magnetic immunochromatography test strip and test kit for quantitative detection of tumor-associated antigen 19-9 in blood

[0034] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:

[0035] A. Antibody preparation: commercialized CA19-9 paired antibody was selected, dialyzed against 20 mM PBS with pH 7.2 (pH 7.0-7.6 is applicable) overnight at 4°C for later use.

[0036] B. Preparation of coating film:

[0037] Preparation of coating buffer: 0.02M phosphate buffer (PB) with pH 7.2 was used as coating buffer, sterilized by 0.22 μm microporous membrane filter and stored at 4°C for later use, valid for two weeks.

[0038] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA, pH 7.2 (pH 7.0-7.6 is applicable), 0.22μm microporous membrane filter sterilization, and store at 4°C for later use , valid for one week.

[0039] Preparation of coating membr...

Embodiment 2

[0068] Except in the step of the preparation of streptavidinized magnetic particles: streptavidin makes the ratio of streptavidin and magnetic particles 1:2, other steps are the same as in Example 1,

Embodiment 3

[0070] Except for the step of preparing streptavidinized magnetic particles: streptavidin makes the ratio of streptavidin to magnetic particles 1:10, other steps are the same as in Example 1.

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Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and a preparation method thereof. A coating film, a magnet particle pad combined with CA19-9 antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A CA19-9 antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology and the biotin-avidin system into the quantitative detection of CA19-9 in blood, and has the advantages that the detection sensitivity and the reliability are greatly improved, the detection is accurate and rapid, long-time waiting is not required, the cost is low, the operation method is simple and convenient and the detection can be controlled easily.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting tumor-associated antigen 19-9 in blood and a preparation method thereof. Background technique [0002] Tumor-associated antigen 19-9 is a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer, also known as pancreatic cancer-associated antigen (GICA). In 1979, Koprowski used the monosialoganglioside isolated from the surface of the human colon cancer cell line sw1116 as an antigen to make the corresponding monoclonal antibody 1116-Ns-19-9, which was used to identify The tumor-associated antigen is called CA19-9. CA19-9 is a mucus-type glycoprotein that exists in the blood and is composed of pancreatic cells, bile ducts, and epithelial cells of the stomach, colon, endometrium, and saliva. Serum cancer antigen 19-9 can be used as an auxiliary diagnostic index for p...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/531G01N33/532G01N33/552
Inventor 唐宝军应希堂李强胡国茂郑金来于尚永
Owner CHEMCLIN DIAGNOSTICS CO LTD
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