31 results about "Biological phenomenon" patented technology

Rapamycin derivatives containing substituents at C-28 in the epi orientation relative to rapamycin are disclosed, together with methods for their preparation and use, e.g. for regulation of biological phenomena in engineered cells. The compounds may contain optional additional modifications relative to rapamycin, as disclosed herein.

It is intended to provide a means of quickly clarifying the dynamics and molecular mechanism of a biological phenomenon by, at least in a single cell, enabling the convenient and quick detection of molecules in a fluid contained in the cell and simultaneously observing the cell dynamics. At the same time, it is aimed at providing a means of searching for an active molecule providing a certain state of a cell from among a number of the detected molecules. The fluid contained in a single cell is directly sucked through a capillary and then loaded directly on an analyzer. This procedure is carried out under a magnifying glass such as a microscope while observing the cell. In the case of employing mass spectrometry, in particular, the fluid contained in the cell is captured by directly sucking through an ionization capillary for a nano-spray, mixed with an ionization-assisting solvent, and then directly introduced into a mass spectrometer by the direct spraying method. Thus, the molecular components of the inner fluid are detected even from a single cell. By analyzing the thus captured molecular peaks among cell data groups in different states with the use of differential analysis or t-calibration, a peak, i.e., a molecule that is specific to a certain state is identified.

Detection sequences of periostin having from 6 to 30 amino acids and which include all or part of peptide sequence SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3, or a homologous peptide sequence. Antibodies suitable for specifically recognizing minimally one of these detection sequences, including an anti-periostin antibody, are disclosed. Periostin detection and dosage processes and kits that use these antibodies are also disclosed. A periostin marker for early diagnosis, tracking and prognosis of pathologies that involve periostin, including benign osteolysis such as inflammatory diseases, including osteoarticular diseases, and malignant osteolysis, such as cancers with bone metastases, is provided.

A method of photoplethysmography includes processing a signal based on at least one signal from at least one sensor arranged to capture light from a living subject to extract information on a characteristic of a periodic biological phenomenon. At least one of the signals from at least one sensor is obtained via sensing radiation as to a peak in an absorption spectrum of water.

The invention relates to a multi-channel mechanical damaging device which is used for damaging cells and which comprises a pin support body 1, a plurality of pins 2 which are arranged in parallel and connected with the pin support body, and a handle which is connected with the pin support body; the length the pins extending out of the pin support body can be adjusted. The pin support body is provided with a plurality of holes 5; the pins can be inserted into the holes and are suitable for the one-time suction head which can be replaced. The multi-channel mechanical damaging device can also be provided with guide components 4 which are arranged on two sides of the pin support body 1; and the angle between the guide components can be regulated. In particular, the invention provides an 8-channel mechanical damaging device which can be used in the cell injury experiment of cells which are cultured by 96-hole microplate. The invention also provides a method which uses the multi-channel mechanical damaging device to damage the cell culture and an application thereof in cell migration and the potential biological phenomena thereof.

The invention discloses application of trans-cucurbit(7)uril in recognition of biogenic amines. Trans-cucurbit(7)uril can be used for recognizing tryptamine by the following steps: (a) adding trans-cucurbit(7)uril into a nuclear magnetism tube, adding D2O, and vibrating to dissolve, so as to obtain a product A; (b) storing tryptamine into a frozen storage pipe, adding D2O to dissolve, so as to obtain a product B; and (c) successively adding the product B into the product A so as to obtain a corresponding nuclear magnetism spectrogram in each dropwise adding process until a series of nuclear magnetism spectrograms of a host-guest complex, making comparison on the nuclear magnetism spectrograms and a nuclear magnetism spectrogram of tryptamine, and determining biogenic amines as tryptamine when two groups of signal peaks Hg and Hf appear in the spectrograms, Hgs move towards a low field, Hfs move to a high field, peak forms of signal peaks of other Ha-He protons are widened and signal peaks move towards the high yield. Trans-cucurbit(7)uril can be used for recognizing six biogenic amines including tryptamine, spermine, spermidine, tyramine and phenylethylamine and is of a great significance to reveal of biological phenomena and processes.

The invention provides a method for calculating degeneracy of suppressed Boolean network. The present invention divides all nodes in the transduction layer into sets, and groups the divided sets, and in each group, calculates the amount of functional contribution overlap between each set and its complement, and calculates the mean value. The sum of the mean values ​​of the overlapping functional contributions of , and the result is the degeneracy of the suppressed Boolean network. The formula for calculating the degeneracy of the suppressed Boolean network is: . The method for calculating the degeneracy of the suppressed Boolean network proposed by the present invention makes the understanding of biological degeneracy rise from qualitative description to quantitative description, and facilitates accurate recognition of degeneracy phenomena in complex biological networks.

Provided herein is a method for determining the temporal progression of a biological phenomenon which may affect a studied subject, the method including the steps of providing first data relative to biomarkers for the studied subject, the biomarkers being relative to the progression of the biological phenomenon, providing a numerical model, converting the first data into at least one point on the same Riemann manifold, and using a numerical model to determine a temporal progression for the biological phenomenon for the studied subject, the numerical model being a function in a Riemann manifold, the numerical model associating to values of biomarkers a temporal progression trajectory for the biological phenomenon and data relative to the dispersion of the progression trajectory for the biological phenomenon among a plurality of subjects, the numerical model being obtained by using a stochastic approximation in an expectation-maximization technique on data relative to biomarkers.

The invention discloses application of trans-cucurbiturils iQ[7] and a preparation method. The trans-cucurbiturils iQ[7] can be used for recognizing lysine, arginine or histidine. The trans-cucurbiturils iQ[7] can effectively recognize lysine, arginine and histidine of 10 amino acids essential for human bodies, has great significances in revealing biological phenomena and process, and has wide application prospects.

The present invention, based on the discovery of a new biological phenomena, provides methods and compositions for use in identifying agents that modulate the phosphorylation of survivin, the interaction between survivin and p34cdc2-cyclin B1 kinase complex, and the interaction between survivin and caspase-9. Related methods and compositions can be used to modulate survivin regulated apoptosis.