Application of a trans seven-membered melon ring to recognize biogenic amines

Abstract

The invention discloses application of trans-cucurbit(7)uril in recognition of biogenic amines. Trans-cucurbit(7)uril can be used for recognizing tryptamine by the following steps: (a) adding trans-cucurbit(7)uril into a nuclear magnetism tube, adding D2O, and vibrating to dissolve, so as to obtain a product A; (b) storing tryptamine into a frozen storage pipe, adding D2O to dissolve, so as to obtain a product B; and (c) successively adding the product B into the product A so as to obtain a corresponding nuclear magnetism spectrogram in each dropwise adding process until a series of nuclear magnetism spectrograms of a host-guest complex, making comparison on the nuclear magnetism spectrograms and a nuclear magnetism spectrogram of tryptamine, and determining biogenic amines as tryptamine when two groups of signal peaks Hg and Hf appear in the spectrograms, Hgs move towards a low field, Hfs move to a high field, peak forms of signal peaks of other Ha-He protons are widened and signal peaks move towards the high yield. Trans-cucurbit(7)uril can be used for recognizing six biogenic amines including tryptamine, spermine, spermidine, tyramine and phenylethylamine and is of a great significance to reveal of biological phenomena and processes.

Description

technical field [0001] The invention relates to the field of supramolecular chemistry, in particular to the application of a trans seven-membered cucurbit ring to recognize biogenic amines. Background technique [0002] Cucurbit[n]uril, also known as cucurbit[n]uril, (abbreviated as CB[n] or Q[n]), named for its structure resembling pumpkin, is a macrocycle following crown ether, cyclodextrin and calixarene. Another new type of cage-like host compound after the compound. From the perspective of structural properties, the cucurbit ring has a highly symmetrical hydrophobic cavity and open and full of polar carbonyl oxygen atoms. The above characteristics of this structure make It can selectively include small molecules such as organic, inorganic and biomolecules of appropriate size in the solution state, or have a hydrophilic coordination with dipole or ionic compounds at the port, and from the function From the point of view of characteristics, cucurbit, as a potential carri...

AI Technical Summary

Problems solved by technology

As an intermediate product controlled by kinetics in the synthesis of cucurbit rings, trans-cucurbitan rings were first reported by the Isaacs and Kim research groups in 2005. In the structure of this type of cucurbit rings, there is an inverted glycoside urea unit, making the The two protons on the waist of the unit point to the cavity of the melon ring, but due to factors such as low content and difficult separation, the development and application of the trans melon ring are limited

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