The invention provides a substrate, a preparation method thereof and a
mass spectrometric analysis detection method for metabolic molecules, and belong to the field of
material synthesis and
mass spectrometry. A
zinc oxide substrate is synthesized by a hydrothermal method with
sodium hydroxide and
zinc acetate as raw materials, conditions are easy to control, the time consumed is short, and the operation is simple. The prepared
zinc oxide substrate is star-shaped nanoparticles assembled by sword-shaped nanorods with sizes of less than 2 microns, particle sizes are uniform, and the substrate has good
ultraviolet absorption properties, thereby having good properties being helpful of
ionization analysis the a sample molecular. Compared with a spherical nano-zinc
oxide substrate, the star-shaped zinc
oxide substrate has smaller
background noise, can remove the background interference and hot spot effect of traditional substrates, meanwhile, has better salt-tolerant and
protein-resistant properties, and can be used for complex biological sample systems such as serums and the like. Simultaneously, sample pretreatment operations such as enrichment, separation and the like are not requiredin the detection process, and the efficient and
rapid detection of the metabolic molecules is achieved.