412 results about "Amino acid mutation" patented technology

The invention discloses a neoantigen prediction method and device based on next-generation sequencing and a storage medium. The method comprises the following steps: obtaining genome sequencing data of a tumor sample and a normal sample, and tumor transcriptome sequencing data; carrying out mutation detection on the genome sequencing data to obtain point mutation and insertion and deletion mutation, and carrying out fusion gene mutation detection on the tumor transcriptome sequencing data to obtain fusion gene mutation; detecting the type of an HLA molecule to obtain an HLA molecule type result, matched with the normal sample, of the HLA molecule of the tumor sample; carrying out annotation from gene mutation to amino acid mutation on the point mutation, the insertion and deletion mutationand the fusion gene mutation; predicting peptide fragments subjected to point mutation, insertion and deletion mutation and fusion gene mutation to obtain corresponding mutation prediction peptide fragments; and inputting the mutation prediction peptide fragments and the HLA molecule type result into a neoantigen prediction model, and performing scoring and sorting through the neoantigen prediction model to obtain a neoantigen prediction result. High-quality neoantigen can be accurately obtained from the sequencing data.