30 results about "Allergen specific IgE" patented technology

Disclosed is a microarray chip for allergy-related immunoglobulin detection, especially for quantitative detection of total IgE and allergen-specific immunoglobulins (such as specific IgE, specific IgG, and specific IgM), which comprises a solid substrate, a reactive layer fabricated on the solid substrate, and at least one allergen or substance capable of binding to immunoglobulin of interest. Whereby, use the result of quantitative detection for allergen-specific IgE to determine hypersensitivity level. In addition, a method for allergy-related immunoglobulin detection using the microarray chip is present, which uses a secondary monoclonal antibody to minimize non-specific binding and applies an enzymatic reaction to amplify reaction signal. An efficient way is thus obtained, which not only reduces time consumption but also provides quantitative measurement.

Methods for performing epitope mapping, and for characterizing the antibody binding affinity and epitope diversity of antibodies in a sample using peptide microarray are provided. In some aspects, methods are provided for the specific characterization of IgE and IgG4. Also disclosed are methods for diagnosing whether a milk-allergic individual will outgrow his or her allergy based on the characterization of the individual's milk allergen-specific IgE antibodies.

The invention discloses a liquid phase chip for detecting an allergen specific antibody. The liquid phase chip mainly comprises (1) a plurality of common allergen probes, comprising 12 fluorescence coded microspheres respectively coated with mite, cockroach, ragweed, felon herb, dog hairs, cat hairs, soybean, peanut, milk, egg, shrimp and crab allergen extracts; (2) a biotin labeling detection antibody; (3) a streptavidin phycoerythrin; (4) a total IgE (immunoglobulin E) probe used when semiquantitative or quantitative detection is carried out on the allergen specific IgE antibody, namely, a fluorescence coded microsphere coated with a rat anti-human IgE monoclonal antibody. The invention also discloses a preparation method of the liquid phase chip probe. The liquid phase chip disclosed by the invention is mainly used for detecting the allergen specific IgE antibody in a serum sample, also for detecting an allergen specific IgG4 (Intravenous Gamma Globulin 4) antibody and a total IgE antibody, and has the advantages of few used samples, parallel detection of a plurality of indexes, high flux, high sensitivity and the like.

The present invention relates to a rapid allergic disease allergen diagnosis kit through a two-step method, a preparation method and a use method thereof. During use of the kit, a serum sample requiring detection and catalase-labeled allergen are subjected to equal volume mixing, room temperature incubation is performed for 5-15 min, the mixed sample is added to a sample hole of colloidal gold test paper strip, and the result is read after 5-15 min, wherein the test strip comprises the sample hole, a colloidal gold pad, a film strip and a water suction pad, colloidal gold is conjugated with biotin labeled goat anti-catalase antibody through streptomycin protein and mouse anti-human IgE monoclonal antibody is coated on the film strip so as to form the colloidal gold-streptomycin protein-biotin-goat anti-catalase antibody-catalase-allergen-specific human IgE antibody-mouse anti-human IgE antibody complex, and the quality control zone is coated with mouse anti-goat IgG antibody. The total detection time of the kit is 10-30 min. The kit has characteristics of strong specificity, high sensitivity, simpleness, rapidness, on-site inspection and no requirement of professional training on operators, wherein operations on allergen specific IgE detection reagents can be completed according to the instruction.

The invention discloses a kit detecting dermatophagoides-farinae allergen specific IgE antibody and a method. The kit comprises a calibration substance, a quality-control substance, a biotin-labeled dermatophagoides-farinae allergen solution, an alkaline-phosphatase-labelled mouse anti-human IgE secondary-antibody solution and a streptavidin-labeled nanometer-magnetic-particle suspension. The kit is utilized to detect the dermatophagoides-farinae allergen specific IgE antibody. Through the above manner, the reagent compositions in the kit are good in stability, the expiration data is one year or more, the detection sensitivity is high, the specificity is good, and variation is small. An improved unified technology is obtained after a large amount of experiments are performed for technology optimization, and production is performed strictly according to standard production operation rules and quality control rules. A user can perform standard operation and obtain a reliable result only according to the operation instruction. During clinic research, the coincidence correlation with a foreign imported reagent is up to 90% or more, and the cost only is 1 / 2 of the foreign imported reagent.

The present invention relates to a rapid allergic disease allergen diagnosis kit through a two-step method, a preparation method and a use method thereof. During use of the kit, a serum sample requiring detection and catalase-labeled allergen are subjected to equal volume mixing, room temperature incubation is performed for 5-15 min, the mixed sample is added to a sample hole of colloidal gold test paper strip, and the result is read after 5-15 min, wherein the test strip comprises the sample hole, a colloidal gold pad, a film strip and a water suction pad, colloidal gold is conjugated with biotin labeled goat anti-catalase antibody through streptomycin protein and mouse anti-human IgE monoclonal antibody is coated on the film strip so as to form the colloidal gold-streptomycin protein-biotin-goat anti-catalase antibody-catalase-allergen-specific human IgE antibody-mouse anti-human IgE antibody complex, and the quality control zone is coated with mouse anti-goat IgG antibody. The total detection time of the kit is 10-30 min. The kit has characteristics of strong specificity, high sensitivity, simpleness, rapidness, on-site inspection and no requirement of professional training on operators, wherein operations on allergen specific IgE detection reagents can be completed according to the instruction.

The invention discloses a liquid phase chip for detecting an allergen specific antibody. The liquid phase chip mainly comprises (1) a plurality of common allergen probes, comprising 12 fluorescence coded microspheres respectively coated with mite, cockroach, ragweed, felon herb, dog hairs, cat hairs, soybean, peanut, milk, egg, shrimp and crab allergen extracts; (2) a biotin labeling detection antibody; (3) a streptavidin phycoerythrin; (4) a total IgE (immunoglobulin E) probe used when semiquantitative or quantitative detection is carried out on the allergen specific IgE antibody, namely, a fluorescence coded microsphere coated with a rat anti-human IgE monoclonal antibody. The invention also discloses a preparation method of the liquid phase chip probe. The liquid phase chip disclosed by the invention is mainly used for detecting the allergen specific IgE antibody in a serum sample, also for detecting an allergen specific IgG4 (Intravenous Gamma Globulin 4) antibody and a total IgE antibody, and has the advantages of few used samples, parallel detection of a plurality of indexes, high flux, high sensitivity and the like.

The invention provides a test strip for screening specific mushroom allergen IgE. The test strip is coated with a mushroom antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, colloidal gold is used for marking specific mushroom allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare the test strip product for allergen detection. The test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

The invention provides a test strip for screening specific weedy pollen allergen IgE (Immunoglobulin E). The test strip is coated by a weedy pollen antigen labeled by colloidal gold. The colloidal gold labelling technology is utilized in the invention; the specific weedy pollen allergen is labelled by using colloidal gold for the first time; then, the labelled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane, therefore, the test strip is prepared for detecting allergens; the test strip disclosed by the invention is simple and convenient to operate, accurate in result and low in cost; and a number of free tests can be realized.

The invention provides a test strip for screening specific cereal allergens IgE. The test strip is coated with a cereal antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, colloidal gold is used for marking specific cereal allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare the test strip product for allergen detection. The test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

The present invention relates to IgE epitope-like peptides which have ability to bind to allergen specific IgE paratopes. Said allergen specific IgEs are bound to effector cells of allergic patients. The IgE epitope-like peptides of the invention cover the paratopes of said IgE bound on effector cells, prevent biding of causative allergen on said IgE on effector cells, and thereby prevent degranulation and secretion of mediators of allergic inflammation from effector cells, after contact with the causative allergen. The present invention relates to the methods of using such IgE epitope-like peptides for therapy of allergic reaction. Said allergic reaction is caused by exposure to the causative allergen.

The invention provides a test strip for screening specific dairy allergen IgE. The test strip is coated with colloidal gold labeled dairy allergen. The colloidal gold labeling technique is utilized, the colloidal gold is used for labeling the specific dairy allergen (dairy antigen) firstly, then the labeled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane in a line manner, and the prepared test strip is used for allergen detection, the operation is simple and convenient, the result is accurate, the cost is low and other free detection can be realized.

The invention provides a test strip for screening specific insulin allergens IgE (Immunoglobulin E). The test strip is coated with an insulin antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific insulin allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

The invention provides a test strip for screening specific animal fur and scurf allergens IgE (Immunoglobulin E). The test strip is coated with an animal fur and scurf antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific animal fur and scurf allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

The invention belongs to the field of biomedicine and relates to a method for detecting serum allergen-specific IgE based on a microfluidic chip and a protein chip. The present invention utilizes microfluidic microfluidic and rapid analysis capabilities, high-throughput analytical capabilities of protein chips, and the principle of specific immune reactions combined with antigens and antibodies, and through the combination of microfluidic chips and protein chips, it can treat clinical serum of allergic patients. The samples were tested for allergen-specific IgE, and the results showed that this method can effectively reduce the amount of serum testing used by patients, and is suitable for patients with a small amount of peripheral blood such as infants or children, while reducing the amount of testing reagents used and testing Time, compared with the existing technology, has the characteristics of rapidity, high throughput, saving detection serum, high efficiency, portability, and low cost. Especially suitable for large-scale epidemiological analysis.

The invention provides a test strip for screening specific silk allergen immunoglobulin E (IgE). The test strip is enveloped by silk antigen labeled by colloidal gold. A colloidal gold-labeled technology is utilized, the specific silk allergen is labeled by the colloidal gold for the first time, and the labeled allergen is evenly sprayed onto a cellulose acetate membrane or a nitrocellulose membrane, so that the prepared test strip can be used for detecting the allergen. The test strip is easy and convenient to operate, accurate in results and low in cost, and is capable of realizing multiple kinds of free detection.

The invention provides a test strip for screening specific albumen allergens IgE (Immunoglobulin E). The test strip is coated with an albumen antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific albumen allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.