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Kit and method for achieving highly-sensitive multiterm joint inspection of allergen-specific IgE antibodies

An allergen and kit technology, which is applied to the kits and fields of highly sensitive multiple joint detection of allergen-specific IgE antibodies, to achieve the effect of less sample consumption and lower dosage

Inactive Publication Date: 2015-11-11
SUZHOU HAOOUBO BIOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, ImmunoCAPRapid, a product of the Swedish Pharmacia company, can only screen a dozen kinds of allergens at a time. It is not equipped with a reader. The sensitivity of the naked eye to interpret the concentration of sIgE antibodies is only 1.0IU / ml (1IUIgE=2.44ngIgE), and 1.49IU / ml is used to distinguish negative and positive result

Method used

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  • Kit and method for achieving highly-sensitive multiterm joint inspection of allergen-specific IgE antibodies

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Provided is a high-sensitivity kit for multiple joint detection of allergen-specific IgE antibodies, including a membrane strip, a polymerase-coupled anti-human IgE antibody, and a substrate chromogen matching the polymerase-coupled anti-human IgE antibody. The film strip is a fibrous material film cured with str-bio-allergen. The polymerase-coupled anti-human IgE antibody is commercially available, and used to prepare a working solution with a concentration of 1:100-10,000 with PBS containing 0.05% TritonX-100 at a pH of 7.4. The substrate chromogenic reagent corresponding to the anti-human IgE antibody coupled to the polymerase is commercially available or can be prepared by itself. The anti-human IgE antibody is goat anti-human IgE antibody, rabbit anti-human IgE antibody or mouse anti-human IgE antibody, and the polymerase is poly-alkaline phosphatase or poly-horseradish peroxidase. The above are the main components of the reagents, excluding conventional reagents ...

Embodiment 2

[0036] Provide a highly sensitive multiple joint detection method for allergen-specific IgE antibody detection, including the following steps:

[0037] (1) Place the antigen membrane strip in the incubation tank, please put the side with the text mark up; add 1ml of sample buffer (50mMPH7.4 Tris buffer) to each tank, and shake the membrane strip slightly at room temperature for 5 minutes, then suck it off.

[0038] (2) Add 1ml of diluted serum sample to each tank (use the sample diluent to half-dilute the serum sample), and incubate for 30 minutes at room temperature on a rocking shaker.

[0039] (3) Suck off the liquid in the incubation tank. Then, add 1ml of washing buffer (0.05% Tween 20, 50mMPH7.4 Tris buffer, 0.05% sodium azide) to each tank, shake gently on a rocking table at room temperature for 5 minutes, and suck off the liquid in the incubation tank. Repeat the wash 2 more times.

[0040] (4) Suck off the liquid in the incubation tank. Add 1ml of enzyme conjugate...

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Abstract

The invention discloses a kit and method for achieving highly-sensitive multiterm joint inspection of allergen-specific IgE antibodies. The kit comprises a membrane strip, the polymerase coupling anti-human IgE antibodies and a substrate color development agent matched with the polymerase coupling anti-human IgE antibodies; str-bio-allergens are fixed on the membrane strip, and the allergen-specific IgE antibodies are detected by adopting the kit. In this way, According to the kit and method for achieving highly-sensitive multiterm joint inspection of the allergen-specific IgE antibodies, the concentration of the allergen-specific antibodies IgE in the human serum or plasma can be detected highly sensitively, qualitatively or semi-quantitatively and quickly, the allergen usage amount can be reduced, dozens of allergens can be screened for one time, the advantages of being quick, accurate and little in sample usage amount are achieved, and high throughput detection is suitably performed.

Description

technical field [0001] The invention relates to a high-sensitivity detection kit for qualitatively or semi-quantitatively screening or detecting multiple allergen-specific IgE antibody levels in human serum or plasma, and a production method and detection method thereof. Background technique [0002] From 2011 to 2012, the white paper of the World Allergy Organization (WAO) on allergy (also known as anaphylaxis, Allergy) pointed out: "The prevalence of allergic diseases is rising dramatically worldwide in developed and developing countries. Allergies Diseases include allergic asthma, allergic rhinitis, severe allergic reactions, allergic reactions to drugs, food and insects, eczema, urticaria (hay fever) and angioedema, etc. In the past two decades, the number of children suffering from allergic diseases, especially There is a tendency for outbreaks to increase.” The prevalence of food-induced allergic diseases alone reaches 3% to 6%. Allergic diseases are when patients inh...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 郭海侠杨万春熊春光彭会军赵莉莉李庆春
Owner SUZHOU HAOOUBO BIOPHARML
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