The present invention provides a method for subculturing
primate embryonic stem cells, and a method for inducing differentiation of the same
cell into a vascular endothelial
cell and a
blood cell. The present invention provides a method comprising culturing
primate embryonic stem cells in a medium containing a
protein component without using feeder cells and cytokines in a container coated with an
extracellular matrix, detaching colonies of the resulting embryonic stem cells in the presence of a cytodetachment agent, and plating the colonies in the similar medium, and a method comprising culturing
primate embryonic stem cells in a serum-containing or not containing medium in the presence of
cytokine, adhesion-culturing the resulting
embryoid body or embroyid body-analogous cellular aggregate in the presence of a
cytokine to obtain specific precursor cells, and separating non-adherent cells and adherent cells from the specific precursor cells to obtain blood cells and vascular endothelial precursor cells.