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Frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles

A technology of composite nanoparticles and chitosan, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc. Long release time, good stability and simple preparation process

Inactive Publication Date: 2017-10-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, proteins and polysaccharides are two kinds of macromolecules with different properties after all. They are aggregated only through simple self-assembly method, the aggregation effect is poor, and the embedding efficiency of the aggregates on bioactive components is low.

Method used

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  • Frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles
  • Frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles
  • Frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles

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Experimental program
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Effect test

Embodiment 1-6

[0058] Embodiment 1-6 (do not add ultrasonic)

[0059] The ratio of rapeseed active protein and chitosan is shown in Table 1, and the molecular weight of chitosan is 100,000 Daltons.

[0060] The preparation method of rapeseed active protein-chitosan composite nanoparticle loaded curcumin is carried out according to the following steps:

[0061] (1) Dissolving rapeseed active protein in distilled water, adjusting pH=12 while magnetically stirring until the protein is completely dissolved; obtaining a solution of rapeseed active protein with a concentration of (2-10) mg / mL;

[0062] (2) Chitosan is dissolved in 0.1M acetic acid aqueous solution, obtains chitosan solution concentration and is (1-2.5) mg / mL solution;

[0063] (3) step (2) chitosan solution is added dropwise in the step (1) rapeseed active protein solution in the ratio of 1:1 by volume, the mass ratio of rapeseed active protein and chitosan is shown in Table 1, Adjust the pH=5 of the mixed solution.

[0064] (4...

Embodiment 7-10

[0074] Embodiment 7-10 (add ultrasonic)

[0075] Rapeseed active protein: 1 part;

[0076] Chitosan: 1 part, its molecular weight is 100,000 Daltons.

[0077] The preparation method of rapeseed active protein-chitosan composite nanoparticle loaded curcumin is carried out according to the following steps:

[0078] (1) Dissolving rapeseed active protein in distilled water, stirring magnetically, adjusting pH=12, until the protein is completely dissolved, and obtaining a solution with a concentration of rapeseed active protein of 2.5 mg / mL;

[0079] (2) chitosan is dissolved in 0.1M acetic acid aqueous solution, and the chitosan solution concentration obtained is a solution of 2.5mg / mL;

[0080] (3) Step (2) chitosan solution is added dropwise in the step (1) rapeseed active protein solution in a ratio of 1:1 by volume, so that the mass ratio of rapeseed active protein and chitosan is 1: 1, then adjust the pH=5 of the mixed solution;

[0081] (4) Rapidly inject the ethanol so...

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Abstract

The invention discloses a frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles, and relates to the technical field of composite nanoparticles of functional food. The rapeseed protein-chitosan nanoparticles are prepared from the following raw materials in parts by weight: 2-10 parts of active rapeseed protein and 1-2.5 parts of chitosan. The chitosan is added into the active rapeseed protein to change the advanced structure of the active rapeseed protein, so that the structure thereof is opened to expose active groups; meanwhile, the protein and polysaccharide form small aggregates, so that formation of the nanoparticles by the aggregates through hydrophobic interaction is promoted, and a foundation is laid for embedding a bioactive component. In the preparation process of the curcumin-embedded active rapeseed protein-chitosan composite nanoparticles, by a frequency-sweeping ultrasonic processing technology, cross-linking of the protein and the polysaccharide into the aggregates is promoted through the physical force of ultrasonic, so that the formation of the nanoparticles by the aggregates through the hydrophobic interaction is promoted and the foundation is laid for embedding the bioactive component.

Description

technical field [0001] The invention relates to the technical field of medicinal nanocomposite particles, in particular to a method for preparing composite nanoparticle by using rapeseed active protein and chitosan as raw materials, adopting frequency-sweeping ultrasonic treatment and embedding curcumin. Background technique [0002] Rapeseed has become one of the important oil crops in the world and in my country because of its rich oil and high-quality protein. my country has always been a major producer of rapeseed, and its planting area and output rank first in the world at present. Its planting area accounts for more than 30% of the total planting area of ​​oilseed crops in the country; the output of rapeseed oil accounts for more than 35% of the total output of major edible vegetable oils; in 2016 The total national rapeseed output has reached 14 million tons. Rapeseed contains 40-45% oil, 20-25% protein and 25% carbohydrates. It is not only the main oil crop, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L89/00C08K5/132C08J3/28C08J3/24B82Y30/00B82Y40/00
CPCC08L5/08B82Y30/00B82Y40/00C08J3/246C08J3/28C08J2305/08C08J2389/00C08J2405/08C08J2489/00C08L89/00C08K5/132
Inventor 任晓锋陈环侯婷张熙梁秋芳朱文静马海乐钟永梅王彤王丽田雨刘尧刚王志荣
Owner JIANGSU UNIV
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