The invention relates to an immobilized
snake venom blood coagulation factor activator and a preparation method of an activated blood coagulation factor. The immobilized
snake venom blood coagulationfactor activator comprises an
active component and a vector provided with the
active component immobilizedly, wherein the
active component is a
snake venom extract. The preparation method of the activated blood coagulation factor comprises the steps that pig
plasma or cow
plasma is made through a filtering column filled with the immobilized snake
venom blood coagulation factor activator to obtainthe activated blood coagulation factor by collecting
effluent or the immobilized snake
venom blood coagulation factor activator is added into the pig
plasma or the cow plasma for stirring for 1-5 hours to obtain the activated blood coagulation factor through filtering. The immobilized snake
venom blood coagulation factor activator can be used repeatedly, and when the primarily-extracted blood coagulation factor is activated, only the immobilized snake venom blood coagulation factor activator needs to be removed, so that the snake venom component in extraction liquid is removed at the same time; moreover, by means of the method, activation reaction time is shortened, the factor
specific activity after activation is improved, and the activation specificity is high.