Immobilized snake venom blood coagulation factor activator and preparation method of activated blood coagulation factor

Abstract

The invention relates to an immobilized snake venom blood coagulation factor activator and a preparation method of an activated blood coagulation factor. The immobilized snake venom blood coagulationfactor activator comprises an active component and a vector provided with the active component immobilizedly, wherein the active component is a snake venom extract. The preparation method of the activated blood coagulation factor comprises the steps that pig plasma or cow plasma is made through a filtering column filled with the immobilized snake venom blood coagulation factor activator to obtainthe activated blood coagulation factor by collecting effluent or the immobilized snake venom blood coagulation factor activator is added into the pig plasma or the cow plasma for stirring for 1-5 hours to obtain the activated blood coagulation factor through filtering. The immobilized snake venom blood coagulation factor activator can be used repeatedly, and when the primarily-extracted blood coagulation factor is activated, only the immobilized snake venom blood coagulation factor activator needs to be removed, so that the snake venom component in extraction liquid is removed at the same time; moreover, by means of the method, activation reaction time is shortened, the factor specific activity after activation is improved, and the activation specificity is high.

Description

technical field [0001] The invention relates to an immobilized snake venom blood coagulation factor activator and a preparation method of the activated blood coagulation factor. Background technique [0002] Factor X and factor II are the two most important coagulation factors in the coagulation cascade system, widely used in coagulation diagnostic reagents, such as thrombin time determination reagents, fibrinogen diagnostic reagents, antithrombin III diagnostic reagents, heparin diagnostic reagents reagent. [0003] At present, the X and II factors used in the production of diagnostic reagents are generally derived from pig blood or cow blood. Blood, and calcium ions, cephalin from animals or snake venom are used as activators to convert inactive factors X and II into activated Xa and IIa. [0004] In the literature "Snake Venom Blood Coagulation Components Research Progress in Procoagulant Mechanism and Application, Xin Shubo", it is introduced that snake venoms of Viper...

AI Technical Summary

Problems solved by technology

[0007] In the current technology, traditional activation methods such as calcium ions or cephalin are not effective in activating factor X, the reaction time exceeds 72 hours, the activation yield is less than 60%, and the specificity is poor, often simultaneously activating factor V, Factor VII and Factor IX

However, the use of snake venom extract with good specificity as an activator introduces toxic substances into the product, and it is necessary to further remove excess snake venom through gel chromatography, which increases production costs and control difficulties