The invention relates to a colloidal gold reading instrument, in particular to a colloidal gold portable CCD reading instrument. The reading instrument includes an upper cover, a lower cover, a front upper cover, an image acquisition device, a display unit, a processing system and a power supply module; The image acquisition device is set, the front upper cover is arranged above the notch to close, and an insertion port for the sample to enter the image acquisition device is arranged on the upper cover; The button module is provided with a transparent window, the display unit is arranged below the transparent window, and a processing system is fixedly arranged on the lower cover below the installation port, and the processing system is respectively connected to the image acquisition device, the button module, the display unit and the power supply module. The reading instrument of the invention has the characteristics of small volume, light weight and portability, can independently process data, and achieve the function of quantitative detection.