39results about How to "Strong ability to split" patented technology

The invention discloses a splitter for rock boring, which comprises a chassis, a large arm, a small arm and a control system, and is characterized by further comprising a propelling beam, a hydraulic rock drill, a propelling oil cylinder, a splitting machine and a switching mechanism, wherein the propelling beam is connected with one end of the small arm through an angle adjusting device; both the hydraulic rock drill and the propelling oil cylinder are arranged on the propelling beam; the propelling oil cylinder drives the hydraulic rock drill to slide on the propelling beam; the switching mechanism is connected to one side edge of the propelling beam; the splitting machine is arranged on the switching mechanism. The splitter is high in efficiency and high in splitting effect.

The invention provides a Dangshan pear genetic transformation method. The Dangshan pear genetic transformation method comprises the following steps: (1) constructing a genetic transformation receptor; (2) culturing agrobacterium tumefaciens and preparing infection solution; (3) carrying out infection and coculture; (4) carrying out sterilization culture and screening culture; and (5) carrying out resistant callus GUS (glucuronidase) staining identification. The Dangshan pear genetic transformation method has the advantages that Dangshan pear callus is taken as the receptor, NptII is taken as a marker gene, GUS gene is taken as a reporter gene, Dangshan pear genetic transformation is carried out by adopting an agrobacterium EHA105 mediated process for obtaining positive resistant callus, and then redifferentiation is carried out by virtue of the positive resistant callus for obtaining test-tube plantlets; meanwhile, a method for carrying out genetic transformation by firstly inducing resistant callus by virtue of fresh treetop, taken as an explant, grown the same year of Dangshan pear is firstly established, content of phenolic substances in fresh treetops of the Dangshan pear in spring is low, less germs are carried, both inoculation browning rate and contamination rate are low, materials are available, callus induction ratio is high, and demand of genetic transformation can be met.

The invention discloses a manual splitter which comprises a splitting mechanism, wherein the splitting mechanism is connected with a manual drive mechanism used for driving the splitting mechanism to operate. The manual splitter has the advantages of being simple and compact in structure, being handy to carry by a single person, being manually driven through manpower, and being especially suitable for post-disaster emergency rescue, barrier breakage and other special occasions.

Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.

The embodiment of the invention provides a partition splitting method. A data file in a target source partition is divided into more than two data groups; one data group is taken as a target split data group; the target split data group is split according to a preset splitting rule and then assigned to a corresponding new partition; after splitting of the target split data set is completed, one data set is selected from the remaining data sets again to serve as the target split data set, splitting of all the data sets is completed through multiple rounds, consumption of resources caused by partition splitting is dispersed to multiple time periods, and resources such as CPU, IO and disk space occupied in the splitting process are saved.

The invention discloses an inducing culture method of an Aconitum vilmorinianum Kom. embryoid. The inducing culture method includes the steps that bulbils of Aconitum vilmorinianum Kom. are used as explants, after the bulbils are disinfected, callus is first induced, and embryonic callus with the color of light yellow with green and vigorous division is selected from the callus for inducing culture, and obtain the Aconitum vilmorinianum Kom. embryoid is obtained. A new way is provided for the mass cultivation and rapid propagation of high-quality seedlings of the Aconitum vilmorinianum Kom., and the problems of the shortage of plant resources and seedling resources of the Aconitum vilmorinianum Kom. as well as serious diseases and the like can be effectively solved.

The invention relates to the technical field of human cloning seedling of plants, in particular to a root-grafting seedling method for Chinese flowering crabapple. Problems that the Chinese flowering crabapple is prone to generation of variation and losing favorable properties due to adoption of sowing and seedling, rooting rate in raising seedlings by cutting is low, more materials are needed, the shape of a seed tree is damaged due to too much trimming, ornamental value is lowered, stock cultivation needed in seedling by grafting is labor-consuming and time-consuming, reproduction is slow, coefficient is low, technical difficulty in group seedling is high, cost is high and the like are effectively solved.

The invention provides a human alveolar epithelial cell separation and culture method. Collected tissue is surrounded by an activated substrate to effectively grow adhering to the wall, serum ingredients can inhibit division and differentiation of alveolar epithelial cells, and therefore, the cell viability is greatly improved; tissue fragments are subjected to shake culture in a specific gaseousenvironment, damage, caused by ischemia, of the collected tissue sample can be effectively avoided, and subsequent culture operation is facilitated. Ureidohydantoin, creatine and asiaticoside added into a passage culture medium can effectively improve the cell viability and the division capacity, and therefore, cell growth and proliferation are promoted. 3T3 cells serve as the trophoderm, a good growth substrate can be provided for the primary alveolar epithelial cells, growth and division of the alveolar epithelial cells can be effectively promoted, and therefore, the culture effect is greatly improved. Through the method, the viability and the proliferation speed of the alveolar epithelial cells can be remarkably increased, and therefore, high-quality experimental materials are providedfor related research projects.

The invention provides a preparation method and application method of a naphthidine molecularly-imprinted polymer. The preparation method provided by the invention comprises the following steps: dissolving S-1,1-binaphthyl-2-amine and methacrylic acid into a pore-forming agent, then feeding nitrogen for 5 minutes under an ultrasonic condition, and then carrying out pre-polymerization for 10 hours at the temperature of 18 DEG C and at normal pressure, so that the S-1,1-binaphthyl-2-amines and the methacrylic acid fully act so as to form a compound; adding ethylene glycol dimethacrylate and azo-bis-iso-butyrynitrile, and then feeding nitrogen for 5 minutes under the ultrasonic condition; placing the obtained product in a water bath kettle at the constant temperature of 60 DEG C to polymerize for 12 hours so as to obtain a bulk molecularly-imprinted polymer; crushing and grinding the obtained bulk molecularly-imprinted polymer; sieving with a sieve of 60mu m-20mu m meshes, and then carrying out repeated settlement with acetone to remove ultra-fine particles; and drying so as to obtain the naphthidine molecularly-imprinted polymer. As a HPLC (high performance liquid chromatography) stationary phase, the imprinted polymer can achieve the chiral separation of racemic DABN (1,1-binaphthyl-2-amine), and the separation factor of the imprinted polymer on the racemic DABN can reach 2.4. The preparation method provided by the invention is high in separation performance, low in cost, simple in operation, and good in stability.

The invention discloses a polyelectrolyte grafted polyvinyl alcohol spinning membrane for oil-water emulsion separation and a preparation method and application thereof.The preparation method comprises the steps that a polymer is dissolved in dimethyl sulfoxide, hexamethylene diisocyanate is added, a third solution system is formed, a cross-linked polyvinyl alcohol spinning membrane is immersed in the third solution system, and the polyelectrolyte grafted polyvinyl alcohol spinning membrane is obtained; treating at a first predetermined temperature for a third predetermined time to obtain a grafted polyvinyl alcohol spinning membrane, and extracting, cleaning and drying the grafted polyvinyl alcohol spinning membrane by using hot dimethyl sulfoxide after the treatment is completed to obtain the polyelectrolyte grafted polyvinyl alcohol spinning membrane. According to the method, emulsion demulsification and oil-water separation operation are combined, an oil-water mixture and oil-water emulsion can be efficiently separated at a high speed, pollution is not likely to happen, the continuous operation capacity is achieved, the emulsion does not need to be demulsified in advance, other substances are not introduced in the collaborative demulsification process, and further recycling and application of separated oil and water are facilitated.

The invention discloses an improvement method for sugarcane stem tip culture, and belongs to the technical field of agricultural production. The improvement method comprises the following steps: a stem tip of a sugarcane tail tip is selected as an explant, heat shock treatment is carried out in sterile water at 40-45 DEG C, the explant is inoculated into an improved MS culture medium, and culturing is performed until a new lateral bud grows; wherein in the culture period, cold stimulation is carried out 1-3 times every day from the fifth day of inoculation, and the cold stimulation lasts for 10-18 days; and the improved MS culture medium comprises the following components: MS, 0.05-0.15 mg / L of thidiazuron, 0.1-0.25 mg / L of 6-BA and 20-30 g / L of cane sugar. According to the improvement method for sugarcane stem tip culture, the highest survival rate of the stem tips reaches 86.35%, the culture period is shortened, and the requirement of the market for a large number of sugarcane asexual propagation materials is met.

The invention discloses a sorbus pohuashanensis stem cell capable of increasing anthocyanin content, a culture medium and a culture method, the culture medium can be applied to the culture for synthesizing anthocyanin from the sorbus pohuashanensis stem cell, and the culture method comprises the following specific steps: (1) preparing stem cell tissues; and (2) induction and accumulation of anthocyanin. According to the method, the konjac is used for preparing a natural culture medium, and nutrient substances of the konjac are used for providing macroelements, microelements and a carbon source for stem cells to be cultured; substances contained in the konjak have a promoting effect on stem cell induction, explants expand in 3-5 days, and stem cells are obviously increased in 7-10 days; the konjak culture medium also has the effect of promoting anthocyanin synthesis, and the anthocyanin content is obviously increased; besides, the konjac glucomannan has good coagulability by utilizing the characteristics of the konjac glucomannan, the addition of agar is reduced, the culture cost is saved, and a target product cultured by the natural culture medium is safer and more credible.

The invention belongs to the technical field of flower seed treatment, and particularly discloses a method for improving butterfly orchid seed germination rate and shortening germinating time. The method specifically comprises the following steps: (1) carrying out freezing treatment on butterfly orchid seeds, taking out the treated butterfly orchid seeds, carrying out stir-frying at constant temperature, taking out the butterfly orchid seeds after stir-frying, soaking the butterfly orchid seeds into a ferric nitrate solution, and taking out a filter liquor; (2) carrying out magnetizing treatment, soaking the seeds in seed soaking solution, fishing out the seeds from the filter liquor, and carrying out microwave drying, wherein the seed soaking solution is prepared from the following raw materials: erythritol, polygahatous polysaccharides, lycium barbarum polysaccharide, robiniae, Chinese toon sprouts, purslane, copper chloride and water. Rapid germination of butterfly orchid seeds is effectively promoted, so that the germination rate reaches 88% or more, the butterfly orchid emergence rate and seedling survival rate are effectively improved, the abloom rate of butterfly orchid seedpropagation can also be improved effectively, the flowering period is prolonged, the occurrence of diseases in the growth period of butterfly orchid can be reduced effectively, the cold resistance isstrengthened, vigorous growth at low temperature can also be realized, and the method is easy to manage.

The invention belongs to a blood-related product, and particularly relates to a human hematopoietic product for oral administration or injection. The main components of the human hematopoiesis comprise essential amino acids, trace elements, vitamins, nutrient substances and growth factors. The preparation method comprises the following steps: synthesizing and sub-packaging in a sterile reaction kettle, diluting with normal saline to form a diluent, mixing with glycerol, lecithin and soybean oil emulsion, inputting into the liquid through mixed gas of medical sterile pure O2 and O3 to enable the oxygen partial pressure to reach 105Kpa or above, filling into an infusion bag at equal pressure to prepare the hematopoietic finished product, and after a pyrogen test is qualified, clinically infusing or orally taking the medicine by a human body. The hematopoietic carbonized fluorine-free compound is prepared from a safe and nontoxic lecithin emulsion; the emulsion which has oxygen carrying capacity, is not limited by blood types, can be stored for a long time, is free of mixing of bacteria or viruses and promotes growth of the hematopoietic stem cells is obtained by diluting a diluent of amino acid, trace elements, vitamins, nutrient substances and growth factors and injecting oxygen at overhigh pressure. The growth factors further promote synthesis of hematopoietic stem cells and division of hemoglobin, the blood recovery capacity is improved through the synergistic effect of multiple raw materials, and the composition is especially suitable for ischemia symptoms such as iron deficiency type anemia.

The invention discloses a 5mm-thick coiled material leveling machine which comprises a machine frame support and a transmission case support, wherein an upper longitudinal pressing board and a lower longitudinal pressing board are installed on the machine frame support, upper wallboards and lower wallboards are connected between two ends of the upper longitudinal pressing board and two ends of the lower longitudinal pressing board, draw-in bolts are connected between the upper wallboards and the lower wallboards on two sides, upper driving rollers and lower driving rollers are installed on the upper wallboards and on the lower wallboard, the other ends of the upper driving rollers and the other ends of the lower driving rollers are connected with a transmission case installed on the transmission case support, triangular reinforcing boards are arranged above the upper wallboards and the lower wallboards, a supporting board is fixedly connected between the triangular reinforcing boards on two sides, lifting mechanisms are arranged on two sides of the supporting board respectively, the lifting mechanisms are in transmission connection with lifting motors, and lifting screws of the lifting mechanisms are connected with the upper longitudinal pressing boards in a screwed mode. The 5mm-thick coiled material leveling machine is suitable for uncoil-leveling, sizing and shearing of various coiled steel boards and improves the sort-shearing capacity of the raw material greatly, and the shearing quality of the coiled steel boards is improved obviously.

The invention relates to the technical field of traffic, and discloses an automatic conveying and shearing device for traffic sign manufacturing. The device comprises an operation table, wherein a side plate is arranged on the side face of the operation table, a crankshaft is arranged on the surface of the side plate, a rocker is movably connected to the surface of the crankshaft, a fluted disc is movably connected to the surface of the side plate, a gear is arranged on the lower surface of the fluted disc, a rolling wheel is arranged on the side face of the gear, a ratchet wheel is arranged in the rolling wheel, an auxiliary wheel is arranged in the operation table, and a cam is arranged on the surface of the crankshaft. According to the automatic conveying and shearing device for traffic sign manufacturing, the crankshaft and the rocker are used in a matched mode, the rocker and the fluted disc are used in a matched mode, the gear and the ratchet wheel are used in a matched mode, and the rolling wheel and the auxiliary wheel are used in a matched mode, traffic signs which are not sheared can be automatically and intermittently conveyed at equal intervals, the conveying effect of the traffic signs is good, and the shearing effect of the traffic signs can be improved.

The invention discloses a culture medium box set for promoting in-vitro rapid propagation of zingiber mioga. The culture medium box set is characterized in that an MS culture medium is used as a basic culture medium, and is added with 1.0 to 3.0mg / L of BA (butyl acrylate), 0.01 to 0.02mg / L of TDZ (thidiazuron), 0.04 to 0.06mg / L of NAA (naphthalene acetic acid), 25 to 35g / L of sugarcane and 6.5 to 7.5g / L of agar, so as to obtain the propagation culture medium. The invention also discloses a method using the culture medium box set to promote the in-vitro rapid propagation of the zingiber mioga. The method has the advantages that under the optimizing conditions, after the zingiber mioga is cultured by the method,; 10 to 15 propagation times can be reached; after 30 days, the rooting rate is 100%; the transplanting survival rate is 100%.

The invention relates to a rapid propagation method for corylus chinensis by inducing clumped buds. According to the technical points, the method is mainly characterized in that seedling stem sections and mature embryos of corylus chinensis serve as explants and are planted in initial media, and after the fixed bud induction rate reaches 96.4% or above, the explants are transplanted into a medium for clumped bud induction, wherein one formula of the initial media comprises MS, 1.0 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, the other formula of the initial media comprises MS, 33.0 mg.L<-1> of GA, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC, and the medium for clumped bud induction is prepared from MS, 1.5 mg.L<-1> of KT, 0.05 mg.L<-1> of IBA, 31.0 mg.L<-1> of GA, 0.1 g.L<-1> of tryptone, 5.8 g.L<-1> of agar, 25 g.L<-1> of saccharose and 0.1 g.L<-1> of AC; the germination rate is 92%.

The invention discloses a composition for digesting allelochemicals in semi-substrate covering raw materials and a using method of the composition, and belongs to the field of garden engineering. Thecomposition comprises ground bark particles and a broad-spectrum microorganism combined composting substance. The using method comprises the following steps: S1, nutrient soil is laid on a bottom layer, and plant fibers, a nutrient substance and an original bacterium solution are sequentially added on the surface of the nutrient soil from top to bottom; S2, operators gather the nutrient soil and the composition into a long strip-shaped gathering pile with a width of 1-2.5 m and a height of 1.5-2 m, meanwhile, air-permeable coverage is carried out on the surface of the gathered object, and semi-corrosion treatment is carried out; S3, the operators control the semi-corrosion treatment temperature to be 43-52 DEG C; and S4, the operators replace the surface of the gathered object by a closedcover, decomposition treatment is carried out, and the temperature is controlled to be 60-65 DEG C in the decomposition process. According to the technical scheme, broad-spectrum microorganisms are used for decomposition, so that tree bark is prevented from generating an uncoupling agent, and the damage to plant roots is avoided.

The invention discloses an induction culture method of a rhizoma bletillae embryoid. The induction culture method comprises the following steps: taking a rhizoma bletillae seed as an explant, culturing a protocorm without differentiating cotyledons firstly, then carrying out culturing on the protocorm without differentiating cotyledons to obtain embryogenic tissue cells with strong division rate and green color, and carrying out induction culture on the embryogenic tissue cells to obtain the rhizoma bletillae embryoid finally. The induction culture method provides a new way for mass cultivation and rapid reproduction of good quality seedlings of rhizoma bletillae, and the problem that the rhizoma bletillae plant resources and seedling resources are short at present can be effectively solved.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The invention relates to a tissue culture method for rapid propagation of alum root "tiramisu", and relates to the technical field of plant tissue culture. The present invention takes alum root petiole as explant, involves steps such as aseptic system establishment, callus induction, callus induction to form adventitious buds, adventitious bud proliferation, rooting induction, seedling hardening and transplanting, etc., and establishes a complete set of Tissue culture rapid propagation method of alum root "Tiramisu". This culture method has the advantages of fast propagation speed, high multiplication rate and low pollution rate, and can quickly produce high-quality seedlings in the production process. Commercial development has important practical application value.

The invention provides a human cervix uterus epithelial cell separation and culture method. The method has the advantages that a collected biopsy sample is preserved in a special preservation liquid,so that cell physiological activity and multiplication capacity can be kept effectively; cell growth factors are added into a primary culture medium, so that cell activity can be increased effectively, cell growth can be promoted, and high-quality primary cells; allantoin, creatine and vitexin are added into a passage culture medium, so that cell activity and division ability can be increased effectively, and cell growth and proliferation are promoted; 3T3 cells are used as the nourishing layer, a good growth substrate can be provided for the primary cervix uterus epithelial cells, the growthand division of the cervix uterus epithelial cells are promoted effectively, and the culture effect is increased greatly; by the method, the activity and proliferation speed of the cervix uterus epithelial cell can be evidently increased, and high-quality experiment materials can be provided for related research projects.

The invention discloses an application of nuclear membrane protein knock-down in stem cell transplantation, which specifically comprises the following steps: 1) designing two pairs of siRNA primers aiming at nuclear membrane protein on line, and constructing a lentiviral vector; (2) intervening proliferation and differentiation of the neural stem cells by in-vitro lentivirus-mediated nuclear membrane protein knock-down; and 3), intervening proliferation and differentiation of the neural stem cells by in-vivo lentivirus-mediated nuclear membrane protein knock-down. According to the invention, LBR (nuclear membrane protein) is used for inhibiting division of stem cells and promoting differentiation of the stem cells; neural stem cells may have strong division ability after transplantation, and have potential carcinogenicity. After the nuclear membrane protein is knocked down, the cell division capability is obviously reduced so that the method can be widely used for solving the carcinogenicity problem caused by stem cell transplantation.

The invention belongs to a blood-related product, and particularly relates to artificial blood for oral administration or injection. The main components of artificial blood comprise essential amino acids, trace elements, vitamins and growth factors. The preparation process comprises the following steps: synthesizing and sub-packaging in a sterile reaction kettle, diluting with normal saline to form a diluent, mixing with soybean oil emulsion, inputting into the liquid through mixed gas of medical sterile pure O2 and O3 to enable the oxygen partial pressure to reach more than 100Kpa, and isobarically filling into an infusion bag to obtain an artificial blood finished product, and after the artificial blood finished product is qualified through pyrogen inspection, the artificial blood can be used for clinical human body infusion or oral administration. The artificial blood is free of carbonated fluorine compounds, through the safe and non-toxic soybean oil emulsion, diluted by the diluent of the amino acids, the trace elements, the vitamins and the growth factors, and injected with oxygen under high pressure, the emulsion which has oxygen carrying capacity, is not limited by blood types, can be stored for a long time, is free of mixing of bacteria or viruses and can promote growth of hematopoietic stem cells is obtained, the growth factors further promotes synthesis of the hematopoietic stem cells and splitting of hemoglobin, the various raw materials have a synergistic effect to improve the blood recovery capability, and the artificial blood is especially suitable for ischemia symptoms such as iron deficiency anemia.

The invention belongs to the technical field of plant induced breeding, and particularly relates to a high-yield cold-resistant stylosanthes guianensis culture method and application thereof, and the method comprises the following steps: 1) selecting stylosanthes guianensis seeds as an induced breeding material, and pretreating the seeds; 2) germination culture: sowing stylosanthes guianensis seeds in a seedling culture medium, placing the seedling culture medium in a greenhouse for seedling culture, watering once every 3-4 days during seedling culture, controlling the temperature of the water to be 10-15 DEG C, and performing heat source irradiation after watering; 3) rooting culture: obtaining seedlings after germination, spraying a rooting solution A on the surface of the seedling culture substrate, transferring the seedling culture substrate to a rooting pot for rooting culture, and spraying a rooting solution B after 3-5 days; and 4) growth culture: after the stylosanthes guianensis seedlings grow to 90-110cm and 3-4 days later, spraying a growth solution once, the stylosanthes guianensis with strong growth ability and excellent cold resistance is cultured by the method, the method can be popularized to the southern subtropical region, and the culture method is provided for expanding the utilization range of the stylosanthes guianensis.

The invention discloses a field sampling treatment agent for date palm, comprising reagent A and reagent B, wherein the reagent A includes 1000-1500 times dilution of azoxystrobin, 1000-1500 times dilution of difenoconazole and carbendazim 800-1000-fold dilution, reagent B includes 6-benzylaminoadenine solution with mass concentration of 5-15mg / L and isopentenyl adenine solution with mass concentration of 1-5mg / L; preparation method: (1) Weigh azoxystrobin, difenoconazole and carbendazim, then dilute with water respectively, and mix to obtain reagent A; (2) weigh 6-benzylaminoadenine and isopentenyladenine, then use respectively Dissolve the sodium hydroxide solution, dilute to volume with sterile water, and mix to obtain reagent B. The field sampling treatment agent for the date palm of the invention can significantly reduce the fungal contamination rate and the endophyte contamination rate, so that the explants can maintain high differentiation ability, and it is easier to carry out detoxification treatment in the process of tissue culture, improve cell differentiation ability, and promote explants. Implants induce callus and shorten the induction period.