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The invention provides a derived peptide for a chicken origin antibacterial peptide as well as a preparation method and application thereof. The sequence of the derived peptide is shown as SEQ No.1. The preparation method comprises the following steps: intercepting 20 amino acids of a chicken origin AvBD4 leader peptide, replacing 5th cysteine and 6th, 7th and 12th phenylalanine by amino acid lysine with charges respectively, meanwhile, carrying out amidation on a carboxyl terminal of the antibacterial peptide, and increasing a positive charge to improve the stability of the antibacterial peptide; adopting a solid-phase chemical synthesis method to obtain crude MGW21; and purifying and identifying synthesized polypeptide by using reversed-phase high performance liquid chromatography and electrospray mass spectrometry. The invention provides the application of the antibacterial peptide in preparing a medicament for treating gram-positive bacteria or gram-negative bacteria infectious diseases. The antibacterial peptide is good in amphiphilic property; compared with an antibiotic and Melittin, the antibacterial peptide is high in antibacterial activity and low in cytotoxicity.

The invention discloses a targeting antimicrobial peptide and a preparation method and application thereof. A sequence of the targeting antimicrobial peptide cCF10-C4 is shown in a sequence table SEQ ID No.1. The preparation method comprises the steps that enterococcus faecalis serves as a target bacterium to obtain the targeting antimicrobial peptide cCF10-C4 through design, a series of multi-structural-domain antimicrobial peptide molecules having selectivity to the enterococcus faecalis are established in a heterozygous mode. The design of a series of antimicrobial peptide molecules mainly includes two independent functional areas, namely a sterilization area and an identification area respectively. The targeting antimicrobial peptide has targeting selection capability to the target bacterium. The targeting antimicrobial peptide cCF10-C4 is conductive to reestablishment of microecological balance after treatment.

The invention provides a β-hairpin antibacterial peptide containing asparagine and glycine turn angle and a preparation method. The sequence of the antimicrobial peptide WRFNG of the present invention is shown in SEQ ID No.1. The preparation method of the present invention uses the Asn-Gly corner as the corner unit, forms the mutual attraction of two β-side chains with the interaction of Trp and Arg, and designs the antibacterial peptide template XWYRYZZXWYRY-NH according to the arrangement principle of the β-hairpin structure 2 . X=R, Y=F, ZZ=NG. The application of the antibacterial peptide in the preparation of drugs for treating infectious diseases caused by Gram-positive bacteria and / or Gram-negative bacteria. The therapeutic index of the antimicrobial peptide of the present invention reaches 138.30, which is 1.58 times higher than WRFPG with Pro-Gly corner, and 3.55 times higher than WRFpG with D-Pro-Gly corner, and has the development potential to become a green and efficient antibiotic substitute.