31results about How to "Promotes re-epithelialization" patented technology

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and non-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, duration and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

Suture-based fasteners and fastening methods for soft tissue reconstruction are described that can include a needle tip swaged onto two sutures and loaded onto the end of a curved needle shaft. The needle shaft and tip can be driven through the tissues to be sutured and back out to where the needle tip can make a permanent connection with a locking bead. The needle shaft can be retracted, leaving two sutures extending from the side of the bead. One suture can be of nonabsorbable material and can be brought through the end of the locking bead opposite the tip and pulled to tighten the suture loop. The other suture can be of an absorbable material and can be used to rotate the locking bead under one of the layers of tissue being sutured after the suture loop is tightened. Following rotation, the second suture can be trimmed.

The invention discloses a skin wound dressing with the antimicrobial property and a preparation method of the skin wound dressing, belonging to the technical field of biomedical engineering. The skin wound dressing provided by the invention is of the spongy porous membrane structure, specifically, a sodium alginate fibrous membrane is prepared by taking sodium alginate as a base material through a freeze drying method, then biological antimicrobial peptide Cys-KR12 is fixed to the fibrous membrane through a surface finishing method, and thus the skin wound dressing is prepared. The skin wound dressing prepared by adopting the method provided by the invention is simple in preparation method and easily available in raw materials, the defects that the drug tolerance is generated, the functions are single, and the like after the existing antimicrobial dressing is used for a long term are overcome, and the skin wound dressing provided by the invention has low immunogenicity, good biocompatibility, good antimicrobial property and good induction repairing function, can promote the healing of the wound surface and the growth of epithelial cells, and is beneficial for the re-epithelialization of the wound surface.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and non-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, duration and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

The invention belongs to the technical field of biomedicine, and particularly relates to an exosome hydrogel wound dressing and a preparation method thereof. The exosome hydrogel wound dressing comprises mesenchymal stem cell exosomes and hydrogel, and the hydrogel is composed of pluronic F127 and pluronic F68. The preparation method comprises the following steps: respectively preparing physiological saline solutions of pluronic F127 and pluronic F68 and mesenchymal stem cell exosome physiological saline resuspension, and mixing the solutions at 4 DEG C to obtain the exosome hydrogel wound dressing. Experimental results show that compared with the mode that mesenchymal stem cell exosomes and hydrogel are independently adopted, the wound dressing prepared through the method can better promote regeneration of skin wound cells and shorten the wound healing time.

The invention relates to a magnolia sieboldii compound natural sunscreen cream and a preparation method thereof. By using flavonoid compounds extracted from magnolia sieboldii, pagodatree flower bud and radix scutellariae as main raw materials, the magnolia sieboldii compound natural sunscreen cream has an obvious broad-spectrum strong ultraviolet absorption effect. By compounding flavonoid compound extracting solution of plants such as magnolia sieboldii with mink oil close to body fat and badger fat with the effects of promoting tissue regeneration, protecting skins and relieving pains and keeping wound wet, a novel natural sunscreen cream containing a plant flavonoid capable of absorbing ultraviolet, and animal and plant compound fat composed of heat-preserving, freeze-proofing and split-preventing fatty acids is obtained.

The present invention relates to the use of xanthan gum as re-epithelializing agent and, in particular, to a pharmaceutical formulation comprising xanthan gum as a re-epithelializing active principle eventually mixed with hyaluronic acid. Said use and composition speed up and improve advantageously the formation of newly grown epithelium.

This invention discloses a topical cooling compressive hemostasis and wound healing device and methods thereof for affecting a percutaneous access wound or an acute surgical wound where the device is compatible with physiological, circulatory and cellular microenvironments of the injury and conforming to the injury site anatomy. The device promotes hemostasis, sustains hemostasis and improves wound healing quality by controlling and affecting vasculature motions of the injury site during various wound healing phases. The device incorporates an elastic material compatible with the elastic nature of the injured vessel to promote comfort and safety. The elastic material is further thermally conductive to deliver and transport cooling and a therapeutic agent to the wound site. The device further incorporates an anatomically conforming lateral stabilization component to stabilize the injury sits and its surroundings.

The invention provides a preparation method of antibacterial dressing with a slow release effect and a product thereof. The preparation method of the antibacterial dressing with the slow release effect comprises the steps that 1, carboxymethyl chitosan, macromolecular collagen and a carboxyl-containing antibiotic are mixed; 2, mixed liquid is poured into a mould for standing and drying to obtain an uncross-linked spongy material; 3, the uncross-linked spongy material is soaked in an absolute ethyl alcohol solution containing EDC / NHS for room temperature cross-linking to obtain the antibacterial dressing. According to the method and the product, by using a mode of which carboxyl on the antibiotic and amino on a compound dressing matrix are subjected to covalent bonding to form amido bonds,the condition that the antibiotic is grafted on the dressing matrix is achieved, through slow breakage of the amido bonds on a polymer skeleton, slow release of the antibiotic in the dressing is achieved, and thus the antibacterial dressing which has higher biocompatibility and long-term antibacterial activity and promotes wound healing is prepared.

The present invention relates to the field of wound healing. More specifically, the present invention provides methods and compositions useful for improved wound healing via autologous stem cell mobilization. In one embodiment, a method for improving wound healing in a patient comprises administering to the patient a therapeutically effective amount of a stem cell mobilizer and a low dose of an immunosuppressive agent.

The invention relates to the field of biological products, and discloses epidermal graft as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: preparing a collagen-chitosan porous bracket, inoculating mesenchymal stem cells to the collagen-chitosan porous bracket, and performing induction culture on the mesenchymal stem cells on the collagen-chitosan porous bracket, so as to obtain epithelial cells. By adopting the epidermal graft, regeneration, reconstruction, remediation and heal of a large-area wound skin surface can be remarkably, efficiently and safely promoted.

The invention relates to the technical field of biological medicine, in particular to application of polypeptide in preparation of a product for promoting skin wound healing. The polypeptide provided by the invention is Hst 1-MAD, and the amino acid sequence of the polypeptide is SHREFPFYGDYGS. The Hst 1-MAD promotes wound closure, reepithelization, collagen deposition, angiogenesis and expression of tight junction protein, and remarkably promotes skin wound healing. Meanwhile, the Hst1-MAD actively regulates the conversion from M1 to M2 macrophages and up-regulates an Nrf2 / NQO1 axis, so that a good environment is created for wound healing. The polypeptide provides a new effective substance for preparing a product for promoting skin wound healing.

The invention provides a liquid dressing for promoting wound repair. The liquid dressing comprises an exosome, a film-forming agent and a hemostatic. The invention further discloses a preparation method of the liquid dressing for promoting wound repair. The liquid dressing prepared by the preparation method disclosed by the invention has relatively good hemostatic and antibacterial effects after wound surface film formation, and can effectively promote wound surface repair and accelerate wound healing.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine film-forming gel for treating a shallow bed-sore at ulceration. The traditional Chinese medicine film-forming gel is prepared from the following medicinal raw materials at a certain weight proportion: cortex dictamni, garden balsam stem, houttuynia cordata, lycopus lucidus, euonymus porphyreus, carambola leaf, rhizoma panasis japonici, folium artemisiae argyi, berberis poiretii schneid, lycopodium clavatum, phoenix-tail fern and bearded mallotus leaf. The traditional Chinese medicine film-forming gel has the effects of clearing away heat and toxic materials, removing necrotic tissue, expelling pus, reducing swelling, relieving pain, supporting necrotic tissue and promoting granulation, and is capable of improving microcirculation around the wound, increasing local blood flow, relieving inflammatory edema and local tissue ischemia and anoxia, reducing inflammatory exudation and promoting absorption and dissipation of inflammation; the traditional Chinese medicine film-forming gel is long in action time, good in transdermic absorption effect, high in bioavailability and free of foreign body sensation in skin after being used; the defect that common gel can be taken away by clothes is avoided; meanwhile, the medication part is in an unsealed state, good in breathability and convenient to use; and the compliance of a patient is improved.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine film-forming gel for treating a shallow bed-sore at ulceration. The traditional Chinese medicine film-forming gel is prepared from the following medicinal raw materials at a certain weight proportion: cortex dictamni, garden balsam stem, houttuynia cordata, lycopus lucidus, euonymus porphyreus, carambola leaf, rhizoma panasis japonici, folium artemisiae argyi, berberis poiretii schneid, lycopodium clavatum, phoenix-tail fern and bearded mallotus leaf. The traditional Chinese medicine film-forming gel has the effects of clearing away heat and toxic materials, removing necrotic tissue, expelling pus, reducing swelling, relieving pain, supporting necrotic tissue and promoting granulation, and is capable of improving microcirculation around the wound, increasing local blood flow, relieving inflammatory edema and local tissue ischemia and anoxia, reducing inflammatory exudation and promoting absorption and dissipation of inflammation; the traditional Chinese medicine film-forming gel is long in action time, good in transdermic absorption effect, high in bioavailability and free of foreign body sensation in skin after being used; the defect that common gel can be taken away by clothes is avoided; meanwhile, the medication part is in an unsealed state, good in breathability and convenient to use; and the compliance of a patient is improved.

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and its application in skin wound repair. The preparation method of the salidroside-collagen sponge scaffold comprises: step 1, preparing a collagen mixture; step 2, injecting into a culture plate; and step 3, freeze-drying. The preparation method uses collagen as a natural material and a drug carrier to construct a salidroside-collagen sponge scaffold, and the prepared salidroside-collagen sponge scaffold has the advantages of high interconnectivity and porosity, and thus does not It affects drug release and has the advantage of good skin wound repair effect. And through animal experiments, it has been confirmed that salidroside-collagen sponge scaffold has a good effect on skin wound repair, which can provide reference for basic research and clinical application of wound repair, and its re-epithelialization effect is the best. Therefore, Rhodiola Glycoside-collagen sponge scaffold can promote skin wound repair and skin re-epithelialization.

The invention provides a human umbilical cord mesenchymal stem cell preparation and application thereof. According to the cell preparation, fibrous protein gel is used as a scaffold during cell transplantation, a three-dimensional environment which is more beneficial to survival is provided for hUC-MSCs cells, and the long-time repair promoting effect can be achieved. The invention further relates to the human mesenchymal stem cells with green fluorescence labels and an application of the human mesenchymal stem cells. Particularly, hUC-MSCs are marked by adopting EGFP, so that in-vivo tracing of transplanted cells and exploration of an action mechanism are realized. In addition, EGFP is adopted for hUC-MSCs, and it is verified that the hUC-MSCs cell preparation provided by the invention can remarkably accelerate healing of diabetic wounds and promote reepithelization.

The invention relates to the field of biological products, and discloses a collagen-chitosan tissue engineering scaffold compounded with Tβ4, a preparation method thereof and an application in preparation of skin substitutes. The preparation method of the present invention comprises: preparing collagen-chitosan porous scaffold, and soaking collagen-chitosan porous scaffold in Tβ4 solution, obtains the collagen-chitosan porous scaffold of compound Tβ4; Then the collagen-chitosan porous scaffold of compound Tβ4 The chitosan porous scaffolds were subjected to chemical cross-linking treatment and freeze-drying treatment sequentially. The collagen-chitosan tissue engineering scaffold compounded with Tβ4 of the present invention can significantly and effectively promote the regeneration, reconstruction, repair and healing of diabetic and other skin refractory wounds.

The invention provides a fusion protein. The fusion protein comprises suppressors of gene silencing, 2A polypeptide and a human acid fibroblast growth factor protein. All or part of the human acid fibroblast growth factor protein is a natural human acid fibroblast growth factor protein; or the human acid fibroblast growth factor protein is a fusion protein formed by co-expression or splicing of the natural human acid fibroblast growth factor protein with another fibroblast factor protein, or with another protein which possesses completely different biological functions. The invention also provides the coding gene of the fusion protein, and a preparation method of genetically modified radix salviae miltiorrhizae which is used for high-efficiency expression of the human acid fibroblast factor. The genetically modified radix salviae miltiorrhizae can be used for effective treatment of operative incisions, nerve injuries, body mechanical injuries, diabetic foot, anabrosis, burned and scalded injuries, and the like, and is also applied in developing a new generation of recombinant human acidic fibroblast factor drugs.

The present invention relates to the use of xanthan gum as re-epithelializing agent and, in particular, to a pharmaceutical formulation comprising xanthan gum as a re-epithelializing active principle eventually mixed with hyaluronic acid. Said use and composition speed up and improve advantageously the formation of newly grown epithelium.

The invention provides compounds, compositions, and methods for the treatment of diseases, disorders, or conditions that are modulated by matrix metalloproteinases (MMPs). The compounds can be selective MMP inhibitors, for example, selective inhibitors of MMP-2, MMP-9, and / or MMP-14. The disease, disorder, or condition can include, for example, stroke, neurological disorders, ophthalmological disorders, or wounds, such as chronic wounds or diabetic wounds.

The invention provides a preparation method of an antibacterial dressing with slow-release effect and its product. The preparation method comprises: (1) mixing carboxymethyl chitosan, macromolecular collagen, and carboxyl-containing antibiotics; (2) mixing Pour the solution into the mold, let it stand, and dry to obtain an uncrosslinked spongy material; (3) Soak the uncrosslinked spongy material in an absolute ethanol solution containing EDC / NHS, and crosslink at room temperature to obtain an antibacterial dressing. In the present invention, the carboxyl group on the antibiotic is covalently bonded with the amino group on the composite dressing matrix to form an amide bond to realize the grafting of the antibiotic on the dressing matrix, and the slow breaking of the amide bond on the polymer skeleton realizes the slow dissolution of the antibiotic in the dressing. release, thus making antibacterial dressings with high biocompatibility, long-term antibacterial activity, and promoting wound healing.

The invention discloses a medical hydrogel antibacterial dressing for preventing new wound scars from forming, which comprises a hydrogel hot compress material main body, the inner side surface position of the hydrogel hot compress material main body is fixedly connected with a hydrogel dressing, the inner side position of the hydrogel dressing is provided with a moisture absorption and viscosity reduction protective film, and the moisture absorption and viscosity reduction protective film is fixedly connected with the hydrogel dressing. One side of the hydrogel hot compress material main body is fixedly connected with a first dustproof pad, and the other side of the hydrogel hot compress material main body is fixedly connected with a second dustproof pad. The medical hydrogel antibacterial dressing for preventing new wound scars from forming is high in biocompatibility, not prone to causing stimulation and allergy, skin-friendly, bacteriostatic, perfectly attached to skin, high in water content, constant in temperature and humidity, capable of creating a moist environment suitable for cell growth and new cell wandering, capable of achieving wet-type healing, capable of achieving mild layered long-acting release due to the adoption of a 3D net layer structure and capable of achieving long-acting healing. The content of effective components is higher, a skin protection film is formed, various shapes can be formed, the wound change situation can be observed, and the liquid absorbency is high.

The invention provides a preparation method of stem cell ointment and the ointment prepared by the method. In the present invention, mesenchymal stem cells with immune regulation and tissue regeneration functions are prepared into agglomerates of uniform size under 3D suspension culture conditions, and mixed in a non-biotoxic active energy matrix to prepare an ointment. Stem cells can survive in this ointment at room temperature for up to a week, maintaining biological activity. The stem cell ointment provided by the invention can be directly applied to the damaged skin, and the stem cells therein will migrate into the wound to suppress inflammation and promote tissue regeneration, accelerate wound healing and significantly improve the healing condition, and reduce scar formation. After the repair process is completed, the stem cells will be cleared by the autoimmune system without residue, safe, stable and efficient.