The invention relates to the technical field of biomedical materials, and discloses a preparation method of a
soft tissue repair material, which comprises the following steps: obtaining and detecting an
umbilical cord and a biological
scaffold material, preparing a decellularized biological
scaffold, culturing and amplifying
umbilical cord mesenchymal stem cell spheres, and planting the
stem cell spheres in a substrate layer of the decellularized biological
scaffold. The invention discloses the
soft tissue repair material; and the invention further discloses application of the
soft tissue repair material in preparation of materials for repairing or replacing soft tissues. The
umbilical cord mesenchymal stem cells and the decellularized biological
scaffold material are organically combined, so that non-invasive repair of
skin defects can be realized, the purpose of chronic
refractory large-area
soft tissue reconstruction can be achieved, the anti-adhesion effect, the bacteriostatic effect, wound protection,
pain relief and epithelization starting capability can be fully exerted, the effect and effectiveness durability are higher than those of a traditional method, the
healing time can be shortened, the treatment cost is reduced, operative wounds are avoided, and the
soft tissue repair material has huge potential and advantages in the field of
regenerative medicine.