Preparation method and application of soft tissue repair material

Abstract

The invention relates to the technical field of biomedical materials, and discloses a preparation method of a soft tissue repair material, which comprises the following steps: obtaining and detecting an umbilical cord and a biological scaffold material, preparing a decellularized biological scaffold, culturing and amplifying umbilical cord mesenchymal stem cell spheres, and planting the stem cell spheres in a substrate layer of the decellularized biological scaffold. The invention discloses the soft tissue repair material; and the invention further discloses application of the soft tissue repair material in preparation of materials for repairing or replacing soft tissues. The umbilical cord mesenchymal stem cells and the decellularized biological scaffold material are organically combined, so that non-invasive repair of skin defects can be realized, the purpose of chronic refractory large-area soft tissue reconstruction can be achieved, the anti-adhesion effect, the bacteriostatic effect, wound protection, pain relief and epithelization starting capability can be fully exerted, the effect and effectiveness durability are higher than those of a traditional method, the healing time can be shortened, the treatment cost is reduced, operative wounds are avoided, and the soft tissue repair material has huge potential and advantages in the field of regenerative medicine.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method and application of a soft tissue repair material. Background technique [0002] Umbilical cord mesenchymal stem cells (hUC-MSCs) are a kind of mesenchymal stem cells present in umbilical cord Wharton's jelly and perivascular tissue. The mesenchymal stem cells derived from the umbilical cord have the following advantages: they are convenient to obtain materials, free from moral and ethical disputes, have a large number of cells that can be obtained, have a strong proliferation ability, have a strong immune regulation effect, and the total amount of secreted cell growth factors is also very high, which is convenient for expansion. Increase and passage, and at the same time, there are no problems such as matching and rejection. Therefore, mesenchymal stem cells derived from umbilical cord are extremely suitable for clinical research and application...

AI Technical Summary

Problems solved by technology

[0005] At present, it has been reported in the literature that stem cells are inoculated on the decellularized amniotic membrane for the repair of skin defects, but there are still defects such as incomplete decellularization, small number of stem cells, and low survival rate. Therefore, the inventors innovatively introduced the 3D structure Stem cell spheres, the organic combination of the two, is far more effective than the simple 2D culture method, with a large number of transplanted stem cells and a fixed position

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