Collagen-chitosan tissue engineering scaffold compounded with tβ4 and its preparation method and application

A technology of tissue engineering scaffold and chitosan, which is applied in medical science, prosthesis, etc., can solve the problems of unfavorable skin refractory wound repair process, skin appendage regeneration and low efficiency, so as to promote rapid angiogenesis and re-epithelialization, Improving the efficiency of transplantation and promoting the effect of repair and regeneration

Inactive Publication Date: 2017-09-19
GENERAL HOSPITAL OF PLA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficiency in treating diabetic skin refractory wounds is low, which is not conducive to promoting the repair process of refractory skin wounds and the regeneration of skin appendages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen-chitosan tissue engineering scaffold compounded with tβ4 and its preparation method and application
  • Collagen-chitosan tissue engineering scaffold compounded with tβ4 and its preparation method and application
  • Collagen-chitosan tissue engineering scaffold compounded with tβ4 and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] In the first aspect, the present invention provides a preparation method of a collagen-chitosan tissue engineering scaffold compounded with Tβ4, the method comprising: preparing a collagen-chitosan porous scaffold, and soaking the collagen-chitosan porous scaffold in Tβ4 In the solution, a collagen-chitosan porous scaffold complexed with Tβ4 is obtained; then the collagen-chitosan porous scaffold complexed with Tβ4 is sequentially subjected to chemical cross-linking treatment and freeze-drying treatment.

[0021] In the preparation method of the present invention, the method for preparing the collagen-chitosan porous scaffold is not particularly limited, and may be various preparation methods commonly used in the art. But, the inventor of the present invention finds in research, when preparing collagen-chitosan porous support, control the specific concentration of collagen and chitosan and the pH value of collagen-chitosan solution in collagen-chitosan solution The cumu...

Embodiment

[0038] The following examples will further illustrate the present invention, but do not limit the present invention thereby.

[0039] The experimental methods in the following examples and comparative examples, unless otherwise specified, are conventional methods in the art. The experimental materials used in the following examples and comparative examples are commercially available unless otherwise specified.

[0040] Chitosan powder was purchased from Sigma Company.

[0041] Tβ4 was purchased from ProSpec Corporation.

[0042] The scanning electron microscope was purchased from Hitachi, Japan, model TM100.

[0043] The preparation method of EDC / NHS cross-linking agent is: dissolve EDC, NHS respectively in 95% ethanol solution to concentration to be 33nmol / L and 8nmol / L, obtain EDC solution and NHS solution, then equal volume of EDC solution and NHS solution mix.

preparation example

[0045] This preparation example is used to illustrate the extraction method of collagen powder.

[0046] (1) Take 10 g of fresh bovine tendon, remove the surrounding connective tissue, and cut the bovine tendon into 1-2mm sized tissue pieces.

[0047] (2) Put the tissue block into 0.1% Na 2 CO 3 Soak in the solution for 2h, rinse with distilled water 3 times, 10min each time.

[0048] (3) Soak the tissue block in 0.5% Tris-HCl (pH=7.5) solution containing 1 mol / L NaCl for 12 hours, rinse with distilled water for 3 times, 10 minutes each time.

[0049] (4) Put the tissue pieces into 250 mL of 0.05 mol / L acetic acid solution containing 500 mg of pepsin, soak at 4°C for 72 hours, and stir repeatedly until fully digested into a transparent paste.

[0050] (5) The mixture was centrifuged at 5000 g for 20 min at 4° C., and the supernatant was taken.

[0051] (6) Perform the operations of steps (4) and (5) on the centrifuged sediment in step (5), and repeat the operation 2 times....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of biological products, and discloses a collagen-chitosan tissue engineering scaffold compounded with Tβ4, a preparation method thereof and an application in preparation of skin substitutes. The preparation method of the present invention comprises: preparing collagen-chitosan porous scaffold, and soaking collagen-chitosan porous scaffold in Tβ4 solution, obtains the collagen-chitosan porous scaffold of compound Tβ4; Then the collagen-chitosan porous scaffold of compound Tβ4 The chitosan porous scaffolds were subjected to chemical cross-linking treatment and freeze-drying treatment sequentially. The collagen-chitosan tissue engineering scaffold compounded with Tβ4 of the present invention can significantly and effectively promote the regeneration, reconstruction, repair and healing of diabetic and other skin refractory wounds.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a Tβ4-composite collagen-chitosan tissue engineering scaffold and its preparation method and application. Background technique [0002] Because the tissue cells of diabetic patients are in a high-glucose environment for a long time, the local skin tissue cells and proteins are glycosylated, causing abnormal vascular endothelial function and impaired response; the function of macrophages is weakened, and the inflammatory response is prolonged and unbalanced; growth The lack or damage of factors, the reduction of collagen tissue, the disorder of epidermal barrier function, and the reduction of granulation tissue make the ulcer difficult to repair. And the research on the treatment of refractory skin wounds caused by diabetes has not yet achieved a major breakthrough. [0003] The collagen-chitosan tissue engineering scaffold based on collagen and chitosan is a relatively complet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/54A61L27/60
Inventor 韩为东付小兵郝好杰遆冬冬佟川郑敬曦
Owner GENERAL HOSPITAL OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap